{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T06:18:55Z","timestamp":1747203535366,"version":"3.40.5"},"posted":{"date-parts":[[2019,7,24]]},"group-title":"Protocol Exchange","reference-count":0,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T00:00:00Z","timestamp":1563926400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2019,7,22]]},"abstract":"<title>Abstract<\/title>\n        <p>This protocol details the experimental procedure for performing the comet assay, a very sensitive DNA break assay based on single cell gel electrophoresis.The analysis of DNA strand breaks, both single- and double-strand breaks (SSBs and DSBs, respectively), was performed in immune responsive cells. The cell line used was the human monocytic cell line THP-1, an adherent cell type with many known applications in in vitro studies of innate immunity. The comet assay is a robust procedure that allows the accurate and reproducible quantification of DNA damage. Here we describe not only the comet assay step-by-step protocol, but also some important aspects related to troubleshooting.<\/p>","DOI":"10.21203\/rs.2.11936\/v2","type":"posted-content","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T10:54:45Z","timestamp":1563965685000},"source":"Crossref","is-referenced-by-count":0,"title":["Alkaline Comet Assay using the monocytic cell line THP-1"],"prefix":"10.21203","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6506-7829","authenticated-orcid":false,"given":"Ana","family":"Neves-Costa","sequence":"first","affiliation":[{"name":"IGC - Instituto Gulbenkian de Ci\u00eancia"}]},{"given":"Dora","family":"Pedroso","sequence":"additional","affiliation":[{"name":"IGC - Instituto Gulbenkian de Ci\u00eancia"}]},{"given":"Luis F","family":"Moita","sequence":"additional","affiliation":[{"name":"IGC - Instituto Gulbenkian de Ci\u00eancia"}]}],"member":"297","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/protocolexchange.researchsquare.com\/article\/pex-605\/v2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/protocolexchange.researchsquare.com\/article\/pex-605\/v2.html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T11:53:22Z","timestamp":1746186802000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.protocols.io\/view\/alkaline-comet-assay-using-the-monocytic-cell-line-81wgbrb61lpk\/v2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,24]]},"references-count":0,"URL":"https:\/\/doi.org\/10.21203\/rs.2.11936\/v2","relation":{},"subject":[],"published":{"date-parts":[[2019,7,24]]},"subtype":"preprint"}}