{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T06:26:17Z","timestamp":1747203977808,"version":"3.40.5"},"posted":{"date-parts":[[2022,6,22]]},"group-title":"In Review","reference-count":0,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T00:00:00Z","timestamp":1655856000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,2,15]]},"abstract":"<title>Abstract<\/title>\n        <p>The steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose lipopolysaccharide, and the 3-O-methylmannose polysaccharide (MMP) only detected in rapidly growing mycobacteria. The recent identification of a unique MMP methyltransferase implicated the adjacent genes in MMP biosynthesis. We report a wide distribution of this gene cluster in NTM, including slowly growing mycobacteria, which we reveal to produce MMP. Using a combination of MMP purification and chemoenzymatic syntheses of intermediates, we identified the biosynthetic mechanism of MMP, relying on two enzymes that we characterized biochemically and structurally: a unique \u03b1\u2013endomannosidade that hydrolyses MMP into defined-sized mannoligosaccharides that prime the elongation of new daughter MMP chains by a rare \u03b1-(1&gt;4)-mannosyltransferase. Therefore, MMP biogenesis occurs through a partially conservative replication mechanism, and disruption of the mechanism affected mycobacterial growth rate at low temperature.<\/p>","DOI":"10.21203\/rs.3.rs-1362692\/v1","type":"posted-content","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T19:32:42Z","timestamp":1655926362000},"source":"Crossref","is-referenced-by-count":0,"title":["Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides"],"prefix":"10.21203","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9005-8377","authenticated-orcid":false,"given":"Ana","family":"Maranha","sequence":"first","affiliation":[{"name":"University of Coimbra"}]},{"given":"Mafalda","family":"Costa","sequence":"additional","affiliation":[{"name":"University of Coimbra"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7235-317X","authenticated-orcid":false,"given":"Jorge","family":"Ripoll-Rozada","sequence":"additional","affiliation":[{"name":"University of Porto"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2565-6239","authenticated-orcid":false,"given":"Jose","family":"Manso","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Health (i3S-University of Porto)"}]},{"given":"Vanessa","family":"Miranda","sequence":"additional","affiliation":[{"name":"Universidade Nova de Lisboa"}]},{"given":"Vera","family":"Mendes","sequence":"additional","affiliation":[{"name":"CNC - Center for Neuroscience and Cell Biology"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2087-4042","authenticated-orcid":false,"given":"Bruno","family":"Manadas","sequence":"additional","affiliation":[{"name":"University of Coimbra"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7698-1170","authenticated-orcid":false,"given":"Sandra","family":"Macedo-Ribeiro","sequence":"additional","affiliation":[{"name":"IBMC\/i3S"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6854-7278","authenticated-orcid":false,"given":"Rita","family":"Ventura","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade NOVA de Lisboa"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0969-5438","authenticated-orcid":false,"given":"Pedro Jos\u00e9 Barbosa","family":"Pereira","sequence":"additional","affiliation":[{"name":"IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8938-7560","authenticated-orcid":false,"given":"Nuno","family":"Empadinhas","sequence":"additional","affiliation":[{"name":"University of Coimbra"}]}],"member":"297","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/www.researchsquare.com\/article\/rs-1362692\/v1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.researchsquare.com\/article\/rs-1362692\/v1.html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T08:06:51Z","timestamp":1674893211000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.researchsquare.com\/article\/rs-1362692\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,22]]},"references-count":0,"URL":"https:\/\/doi.org\/10.21203\/rs.3.rs-1362692\/v1","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1038\/s42003-023-04448-3","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2022,6,22]]},"subtype":"preprint"}}