{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T01:04:14Z","timestamp":1649207054238},"posted":{"date-parts":[[2021,1,13]]},"group-title":"In Review","reference-count":0,"publisher":"Research Square Platform LLC","license":[{"start":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T00:00:00Z","timestamp":1610496000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n        <jats:p>In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt <jats:italic>Laminaria digitata <\/jats:italic>cell wall, with the consequent release of interesting nutritional compounds. An individual alginate lyase, belonging to the family 7 of polysaccharide lyases (PL7), was shown to be the most efficient in the <jats:italic>in vitro<\/jats:italic> degradation of <jats:italic>L. digitata <\/jats:italic>cell wall. The alginate lyase treatment, compared to the control, released up to 7.11 g\/L of reducing sugars (p &lt; 0.001) and 8.59 mmol\/100 g dried alga of monosaccharides (p &lt; 0.001), and reduced cell wall fluorescence intensity by 39.1% after staining with Calcofluor White (p = 0.001). The alginate lyase treatment also released beneficial monounsaturated fatty acids, particularly 18:1<jats:italic>c<\/jats:italic>9 (p &lt; 0.001), and decreased the release of saturated fatty acids (p = 0.001). However, no liberation of proteins (p &gt; 0.170) or pigments (p &gt; 0.070) was observed. Overall, these results show the ability of an individual alginate lyase, from PL7 family, to partially degrade <jats:italic>L. digitata <\/jats:italic>cell wall under physiological conditions. Therefore, this CAZyme can potentially improve the bioavailability of <jats:italic>L. digitata<\/jats:italic> bioactive compounds for monogastric diets, with further application in feed industry.<\/jats:p>","DOI":"10.21203\/rs.3.rs-141997\/v1","type":"posted-content","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:16:02Z","timestamp":1610669762000},"source":"Crossref","is-referenced-by-count":0,"title":["An Individual Alginate Lyase is Effective in the Disruption of Laminaria Digitata Recalcitrant Cell Wall"],"prefix":"10.21203","author":[{"given":"M\u00f3nica","family":"Costa","sequence":"first","affiliation":[{"name":"University of Lisbon"}]},{"given":"Lu\u00eds","family":"Pio","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]},{"given":"Pedro","family":"Bule","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]},{"given":"V\u00e2nia","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Nzytech (Portugal)"}]},{"given":"Cristina","family":"Alfaia","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]},{"given":"Diogo","family":"Coelho","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]},{"given":"Joana","family":"Br\u00e1s","sequence":"additional","affiliation":[{"name":"Nzytech (Portugal)"}]},{"given":"Carlos","family":"Fontes","sequence":"additional","affiliation":[{"name":"Nzytech (Portugal)"}]},{"given":"Jos\u00e9","family":"Prates","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]}],"member":"8761","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/www.researchsquare.com\/article\/rs-141997\/v1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.researchsquare.com\/article\/rs-141997\/v1.html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T17:20:15Z","timestamp":1616001615000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.researchsquare.com\/article\/rs-141997\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,13]]},"references-count":0,"URL":"https:\/\/doi.org\/10.21203\/rs.3.rs-141997\/v1","relation":{},"subject":[],"published":{"date-parts":[[2021,1,13]]},"subtype":"preprint"}}