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Particularly, at the molecular level, the increased stability of surface MHC-I molecules in monocyte-derived DCs (MoDC) underpins an improved DC: T cell interaction. In this study, we focused on the impact of sialic acid remodeling by treatment with <italic>C. perfringens <\/italic>sialidase on MoDCs' phenotypic and functional characteristics. Our investigation juxtaposes this novel approach with the conventional cytokine-based maturation regimen commonly employed in clinical settings.\nNotably,<italic> C. perfringens<\/italic> sialidase remarkably increased MHC-I levels compared to other sialidases having different specificities, supporting the idea that higher MHC-I is due to the cleavage of specific sialoglycans on cell surface proteins. Sialidase treatment induced rapid elevated surface expression of MHC-I, MHC-II, and CD40 within an hour, a response not fully replicated by 48h cytokine cocktail treatment. These increases were also observable 48h post sialidase treatment. While CD86 and PD-L1 showed significant increases after 48h of cytokine maturation, 48h post sialidase treatment showed a higher increase of CD86 and shorter increase of PD-L1. CCR-7 expression was significantly increased 48h after sialidase treatment but not significantly affected by cytokine maturation. Both treatments promoted higher secretion of the IL-12 cytokine. However, the cytokine cocktail induced a more pronounced IL-12 production. SNA lectin staining analysis demonstrated that the sialic acid profile is significantly altered by sialidase treatment, but not by the cytokine cocktail, which causes only slight sialic acid upregulation. Notably, the lipid-presenting molecules CD1a, CD1b, and CD1c remained unaffected by sialidase treatment in MoDCs, a finding also further supported by experiments performed on C1R cells. Inhibition of endogenous sialidases Neu1 and Neu3 during MoDC differentiation did not affect surface MHC-I expression and cytokine secretion. Yet, sialidase activity in MoDCs was minimal, suggesting that sialidase inhibition does not significantly alter MHC-I related functions. Our study highlights the unique maturation profile induced by sialic acid manipulation in MoDCs. These findings provide insights into the potential of sialic acid manipulation as a rapid immunomodulatory strategy, offering promising avenues for targeted interventions in inflammatory contexts.<\/p>","DOI":"10.21203\/rs.3.rs-3981826\/v1","type":"posted-content","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T07:06:41Z","timestamp":1709017601000},"source":"Crossref","is-referenced-by-count":0,"title":["New insights into the immunomodulatory potential of sialic acid on monocyte-derived dendritic cells"],"prefix":"10.21203","author":[{"given":"Z\u00e9lia","family":"Silva","sequence":"first","affiliation":[{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Jo\u00e3o Amorim","family":"Raba\u00e7a","sequence":"additional","affiliation":[{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Vanessa","family":"Luz","sequence":"additional","affiliation":[{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Rita Adubeiro","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Mariolina","family":"Salio","sequence":"additional","affiliation":[{"name":"University of Oxford"}]},{"given":"Alexandra Couto","family":"Oliveira","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]},{"given":"Pedro","family":"Bule","sequence":"additional","affiliation":[{"name":"University of Lisbon"}]},{"given":"Sebastian","family":"Springer","sequence":"additional","affiliation":[{"name":"Constructor University"}]},{"given":"Paula A","family":"Videira","sequence":"additional","affiliation":[{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]}],"member":"297","reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"924","DOI":"10.3389\/fimmu.2020.00924","article-title":"Dendritic Cells and Their Role in Immunotherapy","volume":"11","author":"Gardner A","year":"2020","unstructured":"Gardner A, de Mingo Pulido \u00c1, Ruffell B (2020) Dendritic Cells and Their Role in Immunotherapy. 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