{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T04:24:28Z","timestamp":1649046268689},"posted":{"date-parts":[[2021,4,20]]},"group-title":"In Review","reference-count":0,"publisher":"Research Square Platform LLC","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,4,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n        <jats:p>Most colorectal cancers (CRC) arise from precursor lesions. We aimed to characterize the mutation profile of CRC precursor lesions in a Brazilian population. In total, 90 FFPE lesions, including 67 adenomas, 7 sessile serrated lesions, and 16 hyperplastic polyps, were analyzed by next-generation sequencing. The genetic ancestry of the patients was estimated. Somatic driver mutations were identified in 66.7% of cases, including alterations in <jats:italic>APC<\/jats:italic> (32.2%), <jats:italic>TP53<\/jats:italic> (20.0%), <jats:italic>KRAS<\/jats:italic> (18.9%), <jats:italic>BRAF<\/jats:italic> (13.3%) and <jats:italic>EGFR<\/jats:italic> (7.8%). Adenomas displayed a higher number of mutations, mainly in <jats:italic>APC<\/jats:italic>, compared to serrated polyps (73.1% vs. 47.8%, p\u2009=\u20090.039). Advanced adenomas had a higher frequency of mutation in <jats:italic>KRAS<\/jats:italic> and <jats:italic>GNAS<\/jats:italic> and a high overall mutation rate than early adenomas (92.9% vs. 59%, p\u2009=\u20090.002). Concerning the serrated pathway, a higher frequency of mutations, mainly in <jats:italic>BRAF<\/jats:italic>, was observed in sessile serrated lesions (85.7%) compared to hyperplastic polyps (31.3%, p\u2009=\u20090.027). A high degree of ancestry admixture was observed in the population, with a predominance of European followed by African components. The mutation profile of Brazilian colorectal precursor lesions exhibits a similar landscape to other populations. These results bestow the knowledge of CRC's biological history and may contribute to a molecular screening approach.<\/jats:p>","DOI":"10.21203\/rs.3.rs-428174\/v1","type":"posted-content","created":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T03:49:43Z","timestamp":1618976983000},"source":"Crossref","is-referenced-by-count":0,"title":["Somatic targeted mutation profiling of colorectal cancer precursor lesions"],"prefix":"10.21203","author":[{"given":"Wellington dos","family":"Santos","sequence":"first","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos"}]},{"given":"Mariana Bisarro dos","family":"Reis","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos"}]},{"given":"Jun","family":"Porto","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos"}]},{"given":"Ana Carolina","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos"}]},{"given":"Marcus","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Department of Pathology, Barretos Cancer Hospital, Barretos"}]},{"given":"Gabriela","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Research and Education Institute, Barretos Cancer Hospital, Barretos"}]},{"given":"Kari","family":"Syrj\u00e4nen","sequence":"additional","affiliation":[{"name":"SMW Consultants Ltd, Kaarina"}]},{"given":"Rui Manuel","family":"Reis","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos"}]},{"given":"Denise","family":"Guimaraes","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos"}]}],"member":"8761","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/www.researchsquare.com\/article\/rs-428174\/v1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.researchsquare.com\/article\/rs-428174\/v1.html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T05:29:21Z","timestamp":1626672561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.researchsquare.com\/article\/rs-428174\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.21203\/rs.3.rs-428174\/v1","relation":{},"subject":[],"published":{"date-parts":[[2021,4,20]]},"subtype":"preprint"}}