{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T06:47:52Z","timestamp":1747205272189,"version":"3.40.5"},"posted":{"date-parts":[[2021,5,5]]},"group-title":"In Review","reference-count":0,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T00:00:00Z","timestamp":1620172800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,4,25]]},"abstract":"<title>Abstract<\/title>\n        <p>Background\n\nCryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. To increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells, several studies have been carried out with the culture of ovarian tissue. The aim of this study was to compare a conventional (2D) culture with an alginate matrix three-dimensional (3D) model for ovarian tissue culture.\nResults\n\nThe ovarian tissue culture within the alginate matrix (3D) was similar to 2D culture, regarding follicular density, follicular cell proliferation and cell apoptosis in follicles and stroma. Stromal cells proliferation was kept stable in conventional culture but decreased in 3D culture (p\u2009=\u20090.001). At 24 hours of culture, cytotoxicity was lower in the 3D model (p\u2009=\u20090.006). As culture time increased, cytotoxicity seemed to be similar. Degradation of the tissue was suggested by the histological score analysis of tissue morphology during 72 hours of culture. Tissue injury was greater (p\u2009=\u20090.01) in 3D culture due to higher interstitial oedema (p\u2009=\u20090.017) and tissue necrosis (p\u2009=\u20090.035).\nConclusion\n\nAccording to our results 3D culture of ovarian tissue has no advantage over 2D culture being more time consuming, difficult to perform and having worse reproducibility.<\/p>","DOI":"10.21203\/rs.3.rs-461911\/v1","type":"posted-content","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T16:50:19Z","timestamp":1620233419000},"source":"Crossref","is-referenced-by-count":0,"title":["The Challenge of Ovarian Tissue Culture: 2D Versus 3D Culture"],"prefix":"10.21203","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1977-5258","authenticated-orcid":false,"given":"Ana Sofia","family":"Pais","sequence":"first","affiliation":[{"name":"Centro Hospitalar e Universit\u00e1rio de Coimbra EPE: Centro Hospitalar e Universitario de Coimbra EPE"}]},{"given":"Sandra","family":"Reis","sequence":"additional","affiliation":[{"name":"Centro Hospitalar e Universit\u00e1rio de Coimbra EPE: Centro Hospitalar e Universitario de Coimbra EPE"}]},{"given":"Mafalda","family":"Laranjo","sequence":"additional","affiliation":[{"name":"UC: Universidade de Coimbra"}]},{"given":"Francisco","family":"Caramelo","sequence":"additional","affiliation":[{"name":"UC: Universidade de Coimbra"}]},{"given":"F\u00e1tima","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro Hospitalar e Universit\u00e1rio de Coimbra EPE: Centro Hospitalar e Universitario de Coimbra EPE"}]},{"given":"Maria Filomena","family":"Botelho","sequence":"additional","affiliation":[{"name":"UC: Universidade de Coimbra"}]},{"given":"Teresa","family":"Almeida-Santos","sequence":"additional","affiliation":[{"name":"Centro Hospitalar e Universit\u00e1rio de Coimbra EPE: Centro Hospitalar e Universitario de Coimbra EPE"}]}],"member":"297","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/www.researchsquare.com\/article\/rs-461911\/v1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.researchsquare.com\/article\/rs-461911\/v1.html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T16:50:19Z","timestamp":1620233419000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.researchsquare.com\/article\/rs-461911\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.21203\/rs.3.rs-461911\/v1","relation":{},"subject":[],"published":{"date-parts":[[2021,5,5]]},"subtype":"preprint"}}