{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Research Square"}],"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T06:54:53Z","timestamp":1747205693622,"version":"3.40.5"},"posted":{"date-parts":[[2021,10,1]]},"group-title":"In Review","reference-count":0,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,8,6]]},"abstract":"<title>Abstract<\/title>\n        <p>Exosomes are small extracellular vesicles (EVs) present in human biofluids that can transport specific disease-associated molecules. Consequently blood-derived exosomes have emerged as important peripheral biomarker sources for a wide range of diseases, among them Alzheimer\u2019s disease (AD). Although there is no effective cure for AD, an accurate diagnosis, relying on easily accessible peripheral biofluids, is still necessary to discriminate this disease from other dementias, test potential therapies and even monitor rate of disease progression. The ultimate goal is to produce a cost-effective and widely available alternative, which can also be employed as a first clinical screen. In this study, EVs with exosome-like characteristics were isolated from serum of Controls and AD cases through precipitation- and column-based methods, followed by mass spectrometry analysis. The resulting proteomes were characterized by Gene Ontology (GO) functional enrichment and multivariate analyses. Although GO terms were similar for exosomes proteomes of Controls and ADs, using both methodologies, a clear segregation of disease cases was obtained when using the precipitation-based method. Nine significantly different abundant proteins were identified between Controls and AD cases, representing putative biomarker candidate targets. Among them AACT and C4BP\u03b1, two A\u03b2-binding proteins, whose exosome levels were further validated in individuals from independent cohorts using antibody-based approaches. The findings discussed represent an important contribution to the identification of novel exosomal biomarker candidates useful as potential blood-based tools for AD diagnosis.<\/p>","DOI":"10.21203\/rs.3.rs-787466\/v2","type":"posted-content","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T19:35:25Z","timestamp":1633116925000},"source":"Crossref","is-referenced-by-count":0,"title":["Novel exosome biomarker candidates for Alzheimer\u00b4s disease unraveled through Mass Spectrometry analysis"],"prefix":"10.21203","author":[{"given":"T\u00e2nia Soares","family":"Martins","sequence":"first","affiliation":[{"name":"IBIMED: Universidade de Aveiro Instituto de Biomedicina"}]},{"given":"Rui","family":"Mar\u00e7alo","sequence":"additional","affiliation":[{"name":"IBIMED: Universidade de Aveiro Instituto de Biomedicina"}]},{"given":"Crist\u00f3v\u00e3o B. da Cruz e","family":"Silva","sequence":"additional","affiliation":[{"name":"LIP: Laboratorio de Instrumentacao e Fisica Experimental de Particulas"}]},{"given":"D\u00e1rio","family":"Trindade","sequence":"additional","affiliation":[{"name":"IBIMED: Universidade de Aveiro Instituto de Biomedicina"}]},{"given":"Jos\u00e9","family":"Catita","sequence":"additional","affiliation":[{"name":"Universidade Fernando Pessoa Faculdade de Ciencias da Saude"}]},{"given":"Francisco","family":"Amado","sequence":"additional","affiliation":[{"name":"Universidade de Aveiro Departamento de Quimica"}]},{"given":"T\u00e2nia","family":"Melo","sequence":"additional","affiliation":[{"name":"Universidade de Aveiro Departamento de Quimica"}]},{"given":"Ilka Martins","family":"Rosa","sequence":"additional","affiliation":[{"name":"IBIMED: Universidade de Aveiro Instituto de Biomedicina"}]},{"given":"Jonathan","family":"Vogelgsang","sequence":"additional","affiliation":[{"name":"Universitatsmedizin Gottingen"}]},{"given":"Jens","family":"Wiltfang","sequence":"additional","affiliation":[{"name":"Universitatsmedizin Gottingen"}]},{"given":"Odete A. B. da Cruz e","family":"Silva","sequence":"additional","affiliation":[{"name":"IBIMED: Universidade de Aveiro Instituto de Biomedicina"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0851-6979","authenticated-orcid":false,"given":"Ana Gabriela","family":"Henriques","sequence":"additional","affiliation":[{"name":"iBiMED - University of Aveiro"}]}],"member":"297","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/www.researchsquare.com\/article\/rs-787466\/v2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.researchsquare.com\/article\/rs-787466\/v2.html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T02:55:39Z","timestamp":1659063339000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.researchsquare.com\/article\/rs-787466\/v2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.21203\/rs.3.rs-787466\/v2","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1007\/s12035-022-02762-1","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2021,10,1]]},"subtype":"preprint"}}