{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:01:05Z","timestamp":1777622465738,"version":"3.51.4"},"reference-count":9,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"Abstract<\/jats:title>\n \n Plant-parasitic nematode species have been reported on several occasions from coastal sand dunes, including\n Longidorus<\/jats:italic>\n and\n Rotylenchus<\/jats:italic>\n species (Vovlas et al., 2008; De Luca et al., 2009; Mateille et al., 2014). In April 2016, 10 soil samples of3 to 4 kg from the rhizosphere of\n Tamarix smyrnensis<\/jats:italic>\n with different vegetation around (viz.\n Elymus farctus<\/jats:italic>\n ,\n Lycium schweinfurthii<\/jats:italic>\n ,\n Crithmum maritimum<\/jats:italic>\n , and\n Arthrocnemum<\/jats:italic>\n sp.) were collected for diagnosis of plant-parasitic nematodes. The area of sampling was a coastal sand dune near the archeological site of Komos, southwest of Crete, Greece. Low soil populations of a needle and a spiral nematode were detected (3 and 8 individuals\/1,000 cm\n 3<\/jats:sup>\n of soil, respectively), which prompted us to undertake a detailed morphological and molecular comparative study with previous reported data. Nematodes were extracted from soil with the wet sieving and decanting method (Cobb, 1918). Morphological and molecular analyses of females identified these species as\n Longidorus kuiperi<\/jats:italic>\n Brinkman, Loof and Barbez, 1987, and\n Rotylenchus eximius<\/jats:italic>\n Siddiqi, 1964. The morphology of\n L. kuiperi<\/jats:italic>\n females (six specimens studied) was characterized by having a slender body; very broad lip region (27 6 1.5 [25 to 30] \u03bcm in width); short, hemispherical tail; body length of (7.1 6 0.8 [6.5 to 8.5] mm); vulva position at 47% to 55% of body length; odontostyle length of (105 6 6.5 [90 to 115] \u03bcm); males very common (but less frequent than females [45%\n vs<\/jats:italic>\n 55%]); tail region with 15 to 20 supplements and bulged terminal cuticle. The morphology of\n R. eximius<\/jats:italic>\n females (four specimens studied) was characterized by having a hemispherical lip region clearly set off; with four annuli; body without longitudinal striations; lateral fields areolated in the pharyngeal region only; stylet 36 to 38 \u03bcm; and broadly rounded tail. The morphology of the isolated nematodes agreed with previous descriptions of\n L. kuiperi<\/jats:italic>\n (Brinkman et al., 1987; De Luca et al., 2009), and\n R. eximius<\/jats:italic>\n (Siddiqi, 1964; Castillo and Vovlas, 2005). A single individual was used for DNA extraction. Primers and polymerase chain reaction conditions used in this research were specified in Cantalapiedra et al. (2013) and Archidona-Yuste et al. (2016), and a single amplicon of 800 and 1,100 bp was obtained and sequenced, respectively. D2-D3 (KX398055-KX398056) and ITS sequence alignments (751 and 648 bp, respectively) from\n L. kuiperi<\/jats:italic>\n (KX398057) showed 98% to 99% similarity, differing in 4, and from 6 to 12 nucleotides, respectively, to other sequences of\n L. kuiperi<\/jats:italic>\n deposited in GenBank from Italy and Spain (AM911623, AM905267-AM905270, respectively),with a query coverage of 99%. Similarly, D2-D3 sequence alignment from\n R. eximius<\/jats:italic>\n (KX398058) showed 100% to 99% to another sequence of\n R. eximius<\/jats:italic>\n deposited in GenBank from Italy and Spain (EU280794, DQ328741), differing in zero to three nucleotides, respectively, and a query coverage of 81%. Phylogenetic analyses using Bayesian Inference placed\n L. kuiperi<\/jats:italic>\n in a highly supported (100%) clade that included all\n L. kuiperi<\/jats:italic>\n sequences deposited in GenBank (Archidona-Yuste et al., 2016), and\n R. eximius<\/jats:italic>\n in a highly supported (100%) clade that included all\n R. eximius<\/jats:italic>\n sequences deposited in GenBank (Cantalapiedra-Navarrete et al., 2013). All identification methods were consistent with\n L. kuiperi<\/jats:italic>\n and\n R. eximius<\/jats:italic>\n . To our knowledge, this is the first report of\n L. kuiperi<\/jats:italic>\n and\n R. eximius<\/jats:italic>\n in Greece. Consequently, all these data suggest that coastal sand dunes in Europe constitute environmental conditions optimal for colonization and development of\n L. kuiperi<\/jats:italic>\n , as previously reported (De Luca et al., 2009). Similarly,\n R. eximius<\/jats:italic>\n has been reported in several Mediterranean countries, including Italy, Morocco, Spain, and Tunisia (Castillo and Vovlas, 2005), and this report extend the geographical distribution of this species.\n <\/jats:p>","DOI":"10.21307\/jofnem-2017-019","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T06:42:45Z","timestamp":1500878565000},"page":"135-135","source":"Crossref","is-referenced-by-count":4,"title":["First Report of\n Longidorus kuiperi<\/i>\n and\n Rotylenchus eximius<\/i>\n from Coastal Sand Dunes in Crete, Greece"],"prefix":"10.2478","volume":"48","author":[{"given":"Emmanuel A.","family":"Tzortzakakis","sequence":"first","affiliation":[{"name":"Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture , N.AG.RE.F., Hellenic Agricultural Organization-DEMETER , P.O. Box 2228 , Heraklion , Crete , Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Cantalapiedra-Navarrete","sequence":"additional","affiliation":[{"name":"Institute for Sustainable Agriculture (IAS) , CSIC , Avenida Men\u00e9ndez Pidal s\/n , C\u00f3rdoba , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Archidona-Yuste","sequence":"additional","affiliation":[{"name":"Institute for Sustainable Agriculture (IAS) , CSIC , Avenida Men\u00e9ndez Pidal s\/n , C\u00f3rdoba , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan E.","family":"Palomares-Rius","sequence":"additional","affiliation":[{"name":"Institute for Sustainable Agriculture (IAS) , CSIC , Avenida Men\u00e9ndez Pidal s\/n , C\u00f3rdoba , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Castillo","sequence":"additional","affiliation":[{"name":"Institute for Sustainable Agriculture (IAS) , CSIC , Avenida Men\u00e9ndez Pidal s\/n , C\u00f3rdoba , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2017,7,21]]},"reference":[{"key":"2026042818373521143_j_jofnem-2017-019_ref_001","doi-asserted-by":"crossref","unstructured":"Archidona-Yuste, A., Navas-Cort\u00e9s,J. A., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., and Castillo, P. 2016. Unravelling the biodiversity and molecular phylogeny of needle nematodes of the genus Longidorus (Nematoda: Longidoridae) in olive and a description of six new species. PLoS One 11:e0147689.10.1371\/journal.pone.0147689472682126808994","DOI":"10.1371\/journal.pone.0147689"},{"key":"2026042818373521143_j_jofnem-2017-019_ref_002","unstructured":"Brinkman, H., Loof, P. A. A., and Barbez, D. 1987. Longidorus dunensis n. sp. and L. kuiperi n. sp. from the sand dune coastal region of the Netherlands (Nematoda: Longidoridae). Revue de N\u00e9matologie 10:299\u2013308."},{"key":"2026042818373521143_j_jofnem-2017-019_ref_003","doi-asserted-by":"crossref","unstructured":"Cantalapiedra-Navarrete, C., Navas-Cort\u00e9s,J. A., Liebanas, G.,Vovlas, N., Subbotin, S. A., Palomares-Rius, J. E., and Castillo, P. 2013. Comparative molecular and morphological characterisations in the nematode genus Rotylenchus: Rotylenchus paravitis n. sp., an example of cryptic speciation. Zoologischer Anzeiger 252:246\u2013268.10.1016\/j.jcz.2012.08.002","DOI":"10.1016\/j.jcz.2012.08.002"},{"key":"2026042818373521143_j_jofnem-2017-019_ref_004","doi-asserted-by":"crossref","unstructured":"Castillo, P., and Vovlas, N. 2005. Bionomics and identification of the genus Rotylenchus (Nematoda: Hoplolaimidae). Pp. 377. Leiden, The Netherlands: Brill Academic Publishers.10.1163\/9789047415077","DOI":"10.1163\/9789047415077"},{"key":"2026042818373521143_j_jofnem-2017-019_ref_005","unstructured":"Cobb, N. A. 1918. Agricultural Technology Circular, Bureau of Plant Industry, United States Department of Agriculture. No. 1, 48 pp."},{"key":"2026042818373521143_j_jofnem-2017-019_ref_006","doi-asserted-by":"crossref","unstructured":"De Luca, F., Landa, B. B., Mifsud, D., Troccoli, A., Vovlas, N., and Castillo, P. 2009. Molecular characterisation of Longidorus kuiperi Brinkman, Loof Barbez, 1987 (Nematoda: Longidoridae) from the Mediterranean Basin. Nematology 11:155\u2013l60.","DOI":"10.1163\/156854108X398534"},{"key":"2026042818373521143_j_jofnem-2017-019_ref_007","doi-asserted-by":"crossref","unstructured":"Mateille, T., Tavoillot, J., Martiny, B., and Fargette, M. 2014. Importance of soil characteristics for plant-parasitic nematode communities in European coastal foredunes. European Journal of Soil Biology 64:53\u201360.10.1016\/j.ejsobi.2014.08.002","DOI":"10.1016\/j.ejsobi.2014.08.002"},{"key":"2026042818373521143_j_jofnem-2017-019_ref_008","doi-asserted-by":"crossref","unstructured":"Siddiqi, M. R. 1964. Rotylenchus eximius n. sp. (Nematoda: Hoplolaiminae) found around almond roots in Tunisia. Nematologica 10:101\u2013104.","DOI":"10.1163\/187529264X00682"},{"key":"2026042818373521143_j_jofnem-2017-019_ref_009","doi-asserted-by":"crossref","unstructured":"Vovlas, N., Subbotin, S. A., Troccoli, A., Li\u00e9banas, G., and Castillo, P. 2008. Molecular phylogeny of the genus Rotylenchus (Nematoda, Tylenchida) and description of a new species. Zoologica Scripta 37:521\u2013537.10.1111\/j.1463-6409.2008.00337.x","DOI":"10.1111\/j.1463-6409.2008.00337.x"}],"container-title":["Journal of Nematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.21307\/jofnem-2017-019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:14:17Z","timestamp":1777414457000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.21307\/jofnem-2017-019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,1]]},"references-count":9,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,7,21]]},"published-print":{"date-parts":[[2016,1,1]]}},"alternative-id":["10.21307\/jofnem-2017-019"],"URL":"https:\/\/doi.org\/10.21307\/jofnem-2017-019","relation":{},"ISSN":["2640-396X"],"issn-type":[{"value":"2640-396X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,1]]}}}