{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:27:14Z","timestamp":1790774834783,"version":"4.1.0"},"reference-count":30,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A survey of entomopathogenic nematodes was conducted in sugar cane crops in a total of 14 soils, and positive results were obtained for strain MC5-2014 in the municipality of Tepalcingo, Morelos, in soil with a sandy loam texture and a pH of 6.4. Species determination was performed via morphological and morphometric techniques by searching for a tubular stoma with a swollen cylindrical pharyngeal body and a metacorpus in the basal part. The range of body length (\n                    <jats:italic>L<\/jats:italic>\n                    ) was 750 to 1200\u2009\u03bcm in females and 720 to 910\u2009\u03bcm in males, while the corresponding maximum widths (\n                    <jats:italic>W<\/jats:italic>\n                    ) of the body were 30 to 60 \u03bcm and 20 to 30\u2009\u03bcm, respectively. Males exhibited bursa with a 1\u2009+\u20091\u2009+\u20093\u2009+\u20093 distribution of papillae, and females exhibited a vulva located at the mid-body. For molecular identification, the ITS region of ribosomal DNA was used. Virulence tests (LC\n                    <jats:sub>50<\/jats:sub>\n                    ) were conducted with\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    , and a value of 4.732 was obtained for infective juveniles (IJs). Taking taxonomic and molecular characteristics into account, the isolate was determined to be\n                    <jats:italic>Oscheius myriophila<\/jats:italic>\n                    . The isolation of this strain represents the first geographic report of\n                    <jats:italic>O. myriophila<\/jats:italic>\n                    in Mexico, and it should be noted that the cultivation of sugar cane occurs with constant application of insecticides, herbicides, fungicides, and fertilizers as well as harvesting activities such as burning of the crop for harvest. The\n                    <jats:italic>O. myriophila<\/jats:italic>\n                    isolate has the potential to be used in the future as a method of biological control in our country.\n                  <\/jats:p>","DOI":"10.21307\/jofnem-2020-073","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T06:05:32Z","timestamp":1598853932000},"page":"1-8","source":"Crossref","is-referenced-by-count":18,"title":["<i>Oscheius myriophila<\/i>\n                    (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode"],"prefix":"10.2478","volume":"52","author":[{"given":"Iveth","family":"del Rocio Castro-Ortega","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma del Estado de Morelos, Laboratorio de Control Biol\u00f3gico, Centro de Investigaci\u00f3n en Biotecnolog\u00eda , Cuernavaca , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Manuel","family":"Caspeta-Mandujano","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda Animal, Facultad de Ciencias Biol\u00f3gicas, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca C.P. 6220 , Morelos , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Su\u00e1rez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Molecular de Plantas, Centro de Investigaci\u00f3n en Biotecnolog\u00eda , Cuernavaca , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guadalupe","family":"Pe\u00f1a-Chora","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda Vegetal, Centro de Investigaciones Biol\u00f3gicas , Cuernavaca , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Augusto","family":"Ram\u00edrez-Trujillo","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Molecular de Plantas, Centro de Investigaci\u00f3n en Biotecnolog\u00eda , Cuernavaca , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Cruz-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n Arenas","family":"Sosa","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico Departamento de Medicina Molecular y Bioprocesos del IBT-UNAM. Av. Universidad 2001, Chamilpa, 62210 Cuernavaca , Morelos , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor Manuel","family":"Hern\u00e1ndez-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma del Estado de Morelos, Laboratorio de Control Biol\u00f3gico, Centro de Investigaci\u00f3n en Biotecnolog\u00eda , Cuernavaca , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"key":"2026042817341107196_j_jofnem-2020-073_ref_001","doi-asserted-by":"crossref","unstructured":"Abebe, E., Jumba, M., Bonner, K., Gray, V., Morris, K. and Thomas, W. K. 2010. An entomopathogenic Caenorhabditis briggsae. Journal Experimental Biology 213:3223\u20133229.","DOI":"10.1242\/jeb.043109"},{"key":"2026042817341107196_j_jofnem-2020-073_ref_002","doi-asserted-by":"crossref","unstructured":"Akhurst, R. J. 1980. Morphological and functional dimorphisms in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoplectana and Heterorhabditis. Journal of General Microbiology 121:303\u2013309.","DOI":"10.1099\/00221287-121-2-303"},{"key":"2026042817341107196_j_jofnem-2020-073_ref_003","unstructured":"Boemare, N., Givaudan, A., Brehelin, M. and Laumond, C. 1997. Symbiosis and pathogenicity of nematode bacterium complexes. Symbiosis 22:2145."},{"key":"2026042817341107196_j_jofnem-2020-073_ref_004","doi-asserted-by":"crossref","unstructured":"Dillman, A. R., Chaston, J. M., Adams, B. J., Ciche, T. A., Goodrich-Blair, H., Stock, S. P. and Sternberg, P. W. 2012. An entomopathogenic nematode by any other name. PLOS Pathogens 8:e1002527.","DOI":"10.1371\/journal.ppat.1002527"},{"key":"2026042817341107196_j_jofnem-2020-073_ref_005","doi-asserted-by":"crossref","unstructured":"Dujardin, F. 1845. 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Journal of Nematology 44:348\u2013360."}],"container-title":["Journal of Nematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.21307\/jofnem-2020-073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T21:49:23Z","timestamp":1777412963000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.21307\/jofnem-2020-073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,1]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,7,28]]},"published-print":{"date-parts":[[2020,1,1]]}},"alternative-id":["10.21307\/jofnem-2020-073"],"URL":"https:\/\/doi.org\/10.21307\/jofnem-2020-073","relation":{},"ISSN":["2640-396X"],"issn-type":[{"value":"2640-396X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,1]]}}}