{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:27:42Z","timestamp":1773307662397,"version":"3.50.1"},"reference-count":36,"publisher":"Japanese Society of Toxicology","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Toxicol. Sci."],"published-print":{"date-parts":[[2015]]},"DOI":"10.2131\/jts.40.193","type":"journal-article","created":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T22:03:26Z","timestamp":1426284206000},"page":"193-209","source":"Crossref","is-referenced-by-count":20,"title":["&lt;i&gt;In silico&lt;\/i&gt; risk assessment for skin sensitization using artificial neural network analysis"],"prefix":"10.2131","volume":"40","author":[{"given":"Kyoko","family":"Tsujita-Inoue","sequence":"first","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. Ltd."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Atobe","sequence":"additional","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. Ltd."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morihiko","family":"Hirota","sequence":"additional","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. Ltd."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Ashikaga","sequence":"additional","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. Ltd."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Kouzuki","sequence":"additional","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. Ltd."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"898","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Adler, S., Basketter, D., Creton, S., Pelkonen, O., van Benthem, J., Zuang, V., Andersen, K.E., Angers-Loustau, A., Aptula, A., Bal-Price, A., Benfenati, E., Bernauer, U., Bessems, J., Bois, F.Y., Boobis, A., Brandon, E., Bremer, S., Broschard, T., Casati, S., Coecke, S., Corvi, R., Cronin, M., Daston, G., Dekant, W., Felter, S., Grignard, E., Gundert-Remy, U., Heinonen, T., Kimber, I., Kleinjans, J., Komulainen, H., Kreiling, R., Kreysa, J., Leite, S.B., Loizou, G., Maxwell, G., Mazzatorta, P., Munn, S., Pfuhler, S., Phrakonkham, P., Piersma, A., Poth, A., Prieto, P., Repetto, G., Rogiers, V., Schoeters, G., Schwarz, M., Serafimova, R., T\u00e4hti, H., Testai, E., van Delft, J., van Loveren, H., Vinken, M., Worth, A. and Zaldivar, J.M. (2011): Alternative (non-animal) methods for cosmetics testing: current status and future prospects-2010. Arch. Toxicol., <b>85<\/b>, 367-485.","DOI":"10.1007\/s00204-011-0693-2"},{"key":"2","doi-asserted-by":"crossref","unstructured":"Aeby, P., Ashikaga, T., Bessou-Touya, S., Schepky, A., Gerberick, F., Kern, P., Marrec-Fairley, M., Maxwell, G., Ovigne, J.M., Sakaguchi, H., Reisinger, K., Tailhardat, M., Martinozzi-Teissier, S. and Winkler, P. (2010): Identifying and characterizing chemical skin sensitizers without animal testing: Colipa\u2019s research and method development program. Toxicol. In Vitro, <b>24<\/b>, 1465-1473.","DOI":"10.1016\/j.tiv.2010.07.005"},{"key":"3","doi-asserted-by":"crossref","unstructured":"Api, A.M., Basketter, D.A., Cadby, P.A., Cano, M.F., Ellis, G., Gerberick, G.F., Griem, P., McNamee, P.M., Ryan, C.A. and Safford, R. (2008): Dermal sensitization quantitative risk assessment (QRA) for fragrance ingredients. Regul. Toxicol. Pharm., <b>52<\/b>, 3-23.","DOI":"10.1016\/j.yrtph.2007.10.008"},{"key":"4","doi-asserted-by":"crossref","unstructured":"Ashikaga, T., Yoshida, Y., Hirota, M., Yoneyama, K., Itagaki, H., Sakaguchi, H., Miyazawa, M., Ito, Y., Suzuki, H. and Toyoda, H. (2006): Development of an<i> in vitro<\/i> skin sensitization test using human cell lines: the human cell line activation test (h-CLAT). I. Optimization of the h-CLAT protocol. Toxicol. In Vitro, <b>20<\/b>, 767-773.","DOI":"10.1016\/j.tiv.2005.10.012"},{"key":"5","doi-asserted-by":"crossref","unstructured":"Azam, P., Peiffer, J.L., Chamousset, D., Tissier, M.H., Bonnet, P.A., Vian, L., Fabre, I. and Ourlin, J.C. (2006): The cytokine-dependent MUTZ-3 cell line as an <i>in vitro<\/i> model for the screening of contact sensitizers. Toxicol. Appl. Pharmacol., <b>212<\/b>, 14-23.","DOI":"10.1016\/j.taap.2005.06.018"},{"key":"6","doi-asserted-by":"crossref","unstructured":"Basketter, D.A., Clapp, C., Jefferies, D., Safford, B., Ryan, C.A., Gerberick, F., Dearman, R.J. and Kimber, I. (2005): Predictive identification of human skin sensitization thresholds. Contact Dermatitis, <b>53<\/b>, 260-267.","DOI":"10.1111\/j.0105-1873.2005.00707.x"},{"key":"7","doi-asserted-by":"crossref","unstructured":"Basketter, D.A., Evans, P., Fielder, R.J., Gerberick, G.F., Dearman, R.J. and Kimber, I. (2002): Local lymph node assay - validation, conduct and use in practice. Food Chem. Toxicol., <b>40<\/b>, 593-598.","DOI":"10.1016\/S0278-6915(01)00130-2"},{"key":"8","doi-asserted-by":"crossref","unstructured":"Basketter, D.A. and Kimber, I. (2011): Predictive tests for irritants and allergens and their use in quantitative risk assessment. In \u201cContact Dermatitis\u201d, 5th edition, chapter 13. Springer, Berlin, Eds Johansen, J.D., Frosch, P.F. and Lepoittevin, J-P., pp.229-240.","DOI":"10.1007\/978-3-642-03827-3_13"},{"key":"9","doi-asserted-by":"crossref","unstructured":"Basketter, D.A., Sanders, D. and Jowsey, I.R. (2007): The skin sensitization potential of resorcinol: Experience with the local lymph node assay. Contact Dermatitis, <b>56<\/b>, 196-200.","DOI":"10.1111\/j.1600-0536.2006.01008.x"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Bauch, C., Kolle, S.N., Ramirez, T., Eltze, T., Fabian, E., Mehling, A., Teubner, W., van Ravenzwaay, B. and Landsiedel, R. (2012): Putting the parts together: combining<i> in vitro<\/i> methods to test for skin sensitizing potentials. Regul. Toxicol. Pharm., <b>63<\/b>, 489-504.","DOI":"10.1016\/j.yrtph.2012.05.013"},{"key":"11","doi-asserted-by":"crossref","unstructured":"Emter, R., Ellis, G. and Natsch, A. (2010): Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers<i> in vitro<\/i>. Toxicol. Appl. Pharmacol., <b>245<\/b>, 281-290.","DOI":"10.1016\/j.taap.2010.03.009"},{"key":"12","doi-asserted-by":"crossref","unstructured":"Fedorowicz, A., Singh, H., Soderholm, S. and Demchuk, E. (2005): Structure-activity models for contact sensitization. Chem. Res. Toxicol., <b>18<\/b>, 954-969.","DOI":"10.1021\/tx0497806"},{"key":"13","unstructured":"Gerberick, G.F., Ryan, C.A., Kern, P.S., Schlatter, H., Dearman, R.J., Kimber, I. Patlewicz, G.Y. and Basketter, D.A. (2005): Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods. Dermatitis, <b>16<\/b>, 157-202."},{"key":"14","doi-asserted-by":"crossref","unstructured":"Gerberick, G.F., Vassallo, J.D., Foertsch, L.M., Price, B.B., Chaney, J.G. and Lepoittevin, J.P. (2007): Quantification of chemical peptide reactivity for screening contact allergens: a classification tree model approach. Toxicol. Sci., <b>97<\/b>, 417-427.","DOI":"10.1093\/toxsci\/kfm064"},{"key":"15","doi-asserted-by":"crossref","unstructured":"Hirota, M., Kouzuki, H., Ashikaga, T., Sono, S., Tsujita, K., Sasa, H. and Aiba, S. (2013): Artificial neural network analysis of data from multiple <i>in vitro<\/i> assays for prediction of skin sensitization potency of chemicals. Toxicol. In Vitro, <b>27<\/b>, 1233-1246.","DOI":"10.1016\/j.tiv.2013.02.013"},{"key":"16","doi-asserted-by":"crossref","unstructured":"Jaworska, J., Harol, A., Kern, P.S. and Gerberick, G.F. (2011): Integrating non-animal test information into an adaptive testing strategy - skin sensitization proof of concept case. ALTEX, <b>28<\/b>, 211-225.","DOI":"10.14573\/altex.2011.3.211"},{"key":"17","doi-asserted-by":"crossref","unstructured":"Jaworska, J., Dancik, Y., Kern, P., Gerberick, F. and Natsch, A. (2013): Bayesian integrated testing strategy to assess skin sensitization potency: from theory to practice. J. Appl. Toxicol., <b>33<\/b>, 1353-1364.","DOI":"10.1002\/jat.2869"},{"key":"18","doi-asserted-by":"crossref","unstructured":"Johansson, H., Albrekt, A.S., Borrebaeck, C.A. and Lindstedt, M. (2013): The GARD assay for assessment of chemical skin sensitizers. Toxicol. In Vitro, <b>27<\/b>, 1163-1169.","DOI":"10.1016\/j.tiv.2012.05.019"},{"key":"19","doi-asserted-by":"crossref","unstructured":"Jowsey, I.R., Basketter, D.A., Westmoreland, C. and Kimber, I. (2006): A future approach to measuring relative skin sensitising potency: a proposal. J. Appl. Toxicol., <b>26<\/b>, 341-350.","DOI":"10.1002\/jat.1146"},{"key":"20","doi-asserted-by":"crossref","unstructured":"Kimber, I., Basketter, D.A., Gerberick, F. and Dearman, R.J. (2002): Allergic contact dermatitis. Int. Immunopharmacol.,<b> 2<\/b>, 201-211.","DOI":"10.1016\/S1567-5769(01)00173-4"},{"key":"21","doi-asserted-by":"crossref","unstructured":"Lambrechts, N., Vanheel, H., Nelissen, I., Witters, H., Van Den Heuvel, R., Van Tendeloo, V., Schoeters, G. and Hooyberghs, J. (2010): Assessment of chemical skin-sensitizing potency by an<i> in vitro<\/i> assay based on human dendritic cells. Toxicol. Sci., <b>116<\/b>, 112-129.","DOI":"10.1093\/toxsci\/kfq108"},{"key":"22","doi-asserted-by":"crossref","unstructured":"Magnusson, B. and Kligman, A.M. (1969): The identification of contact allergens by animal assay. The guinea pig maximization test. J. Invest. Dermatol., <b>52<\/b>, 268-276.","DOI":"10.1038\/jid.1969.42"},{"key":"23","doi-asserted-by":"crossref","unstructured":"Natsch, A. and Emter, R. (2008): Skin sensitizers induce antioxidant response element dependent genes: Application to the <i>in vitro<\/i> testing of the sensitization potential of chemicals. Toxicol. Sci., <b>102<\/b>, 110-119.","DOI":"10.1093\/toxsci\/kfm259"},{"key":"24","doi-asserted-by":"crossref","unstructured":"Natsch, A., Emter, R. and Ellis, G. (2009): Filling the concept with data: integrating data from different <i>in vitro<\/i> and<i> in silico<\/i> assays on skin sensitizers to explore the battery approach for animal-free skin sensitization testing. Toxicol. Sci., <b>107<\/b>, 106-121.","DOI":"10.1093\/toxsci\/kfn204"},{"key":"25","doi-asserted-by":"crossref","unstructured":"Natsch, A., Ryan, C.A., Foertsch, L., Emter, R., Jaworska, J., Gerberick, F. and Kern, P. (2013): A dataset on 145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation. J. Appl. Toxicol., <b>33<\/b>, 1337-1352.","DOI":"10.1002\/jat.2868"},{"key":"26","doi-asserted-by":"crossref","unstructured":"Nukada, Y., Miyazawa, M., Saitou, K., Sakaguchi, H. and Nishiyama, N. (2013): Data integration of non-animal tests for the development of a test battery to predict the skin sensitizing potential and potency of chemicals. Toxicol. In Vitro, <b>27<\/b>, 609-618.","DOI":"10.1016\/j.tiv.2012.11.006"},{"key":"27","unstructured":"OECD (2012): The adverse outcome pathway for skin sensitization initiated by covalent binding to proteins. Part 1: Scientific evidence. OECD environment, health and safety publications series on testing and assessment. <b>168<\/b>, 1-46"},{"key":"28","doi-asserted-by":"crossref","unstructured":"Patlewicz, G., Aptula, A.O., Roberts, D.W. and Uriarte, E. (2008): A minireview of available skin sensitisation (Q)SARs\/Expert systems. QSAR Comb. Sci., <b>27<\/b>, 60-76.","DOI":"10.1002\/qsar.200710067"},{"key":"29","unstructured":"Patlewicz, G. and Worth, A. (2008): Review of data sources, QSARs and integrated testing strategies for skin sensitization. JRC Scientific and technical reports. EUR 23225 EN \u2013 2008. http:\/\/publications.jrc.ec.europa.eu\/repository\/handle\/111111111\/998"},{"key":"30","doi-asserted-by":"crossref","unstructured":"Python, F., Goebel, C. and Aeby, P. (2007): Assessment of the U937 cell line for the detection of contact allergens. Toxicol. Appl. Pharmacol., <b>220<\/b>, 113-124.","DOI":"10.1016\/j.taap.2006.12.026"},{"key":"31","doi-asserted-by":"crossref","unstructured":"Sakaguchi, H., Ashikaga, T., Miyazawa, M., Yoshida, Y., Ito, Y., Yoneyama, K., Hirota, M., Itagaki, H., Toyoda, H. and Suzuki, H. (2006): Development of an<i> in vitro<\/i> skin sensitization test using human cell lines; human cell line activation test (h-CLAT). II. An inter-laboratory study of the h-CLAT. Toxicol. In Vitro,<b> 20<\/b>, 774-784.","DOI":"10.1016\/j.tiv.2005.10.014"},{"key":"32","doi-asserted-by":"crossref","unstructured":"Suzuki, M., Hirota, M., Hagino, S., Itagaki, H. and Aiba, S. (2009): Evaluation of changes of cell-surface thiols as a new biomarker for<i> in vitro<\/i> sensitization test. Toxicol. In Vitro, <b>23<\/b>, 687-696.","DOI":"10.1016\/j.tiv.2009.02.002"},{"key":"33","doi-asserted-by":"crossref","unstructured":"Takahashi, T., Kimura, Y., Saito, R., Nakajima, Y., Ohmiya, Y., Yamasaki, K. and Aiba, S. (2011): An<i> in vitro<\/i> test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8. Toxicol. Sci., <b>124<\/b>, 359-369.","DOI":"10.1093\/toxsci\/kfr237"},{"key":"34","doi-asserted-by":"crossref","unstructured":"Teubner, W., Mehling, A., Schuster, P.X., Guth, K., Worth, A., Burton, J., van Ravenzwaay, B. and Landsiedel, R. (2013): Computer models versus reality: How well do<i> in silico<\/i> models currently predict the sensitization potential of a substance. Regul. Toxicol. Pharm., <b>67<\/b>, 468-485.","DOI":"10.1016\/j.yrtph.2013.09.007"},{"key":"35","doi-asserted-by":"crossref","unstructured":"Tsujita-Inoue, K., Hirota, M., Ashikaga, T., Atobe, T., Kouzuki, H. and Aiba, S. (2014): Skin sensitization risk assessment model using artificial neural network analysis of data from multiple<i> in vitro<\/i> assays. Toxicol. In Vitro, <b>28<\/b>, 626-639.","DOI":"10.1016\/j.tiv.2014.01.003"},{"key":"36","doi-asserted-by":"crossref","unstructured":"Vacanson, M., Achachi, A., Mutez, V., Cluzel-Tailhardat, M., Varlet, B.L., Rozi\u00e8res, A., Fournier, P. and Nicolas, J.F. (2014): Human T cell priming assay: depletion of peripheral blood lymphocytes in CD25(+) cells improves the<i> in vitro<\/i> detection of weak allergen-specific T cells. Experientia Suppl., <b>104<\/b>, 89-100.","DOI":"10.1007\/978-3-0348-0726-5_7"}],"container-title":["The Journal of Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/40\/2\/40_193\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T07:20:21Z","timestamp":1498202421000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/40\/2\/40_193\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015]]}},"URL":"https:\/\/doi.org\/10.2131\/jts.40.193","relation":{},"ISSN":["0388-1350","1880-3989"],"issn-type":[{"value":"0388-1350","type":"print"},{"value":"1880-3989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}