{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:34:33Z","timestamp":1789929273339,"version":"4.0.1"},"reference-count":17,"publisher":"Japanese Society of Toxicology","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Toxicol. Sci."],"published-print":{"date-parts":[[2018]]},"DOI":"10.2131\/jts.43.741","type":"journal-article","created":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T17:36:58Z","timestamp":1543858618000},"page":"741-749","source":"Crossref","is-referenced-by-count":9,"title":["The performance of an &lt;i&gt;in vitro&lt;\/i&gt; skin sensitisation test, IL-8 Luc assay (OECD442E), and the integrated approach with direct peptide reactive assay (DPRA)"],"prefix":"10.2131","volume":"43","author":[{"given":"Yutaka","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Dermatology, Tohoku University Graduate School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Hatano Research Institute, Food and Drug Safety Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Iwaki","sequence":"additional","affiliation":[{"name":"Health Research Institute, Advanced Industrial Science and technology (AIST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohji","family":"Yamakage","sequence":"additional","affiliation":[{"name":"Hatano Research Institute, Food and Drug Safety Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Saito","sequence":"additional","affiliation":[{"name":"Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Health Research Institute, Advanced Industrial Science and technology (AIST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chizu","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Tohoku University Graduate School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Ohmiya","sequence":"additional","affiliation":[{"name":"Biomedical Research Institute, Advanced Industrial Science and Technology (AIST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Omori","sequence":"additional","affiliation":[{"name":"Division of Biostatistics, Department of Social\/Community Medicine and Health Science, Kobe University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Kojima","sequence":"additional","affiliation":[{"name":"Japanese Center for the Validation of Alternative Methods"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Setsuya","family":"Aiba","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Tohoku University Graduate School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"898","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Adler, S., Basketter, D., Creton, S., Pelkonen, O., van Benthem, J., et al. (2011): Alternative (non-animal) methods for cosmetics testing: current status and future prospects-2010. Arch. Toxicol., <b>85<\/b>, 367-485.","DOI":"10.1007\/s00204-011-0693-2"},{"key":"2","first-page":"157","year":"2005","unstructured":"Gerberick, G.F., Ryan, C.A., Kern, P.S., Schlatter, H., Dearman, R.J., Kimber, I., Patlewicz, G.Y. and Basketter, D.A. (2005): Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods. Dermatitis, <b>16<\/b>, 157-202.","journal-title":"Dermatitis","ISSN":"https:\/\/id.crossref.org\/issn\/1710-3568","issn-type":"print"},{"key":"3","doi-asserted-by":"crossref","unstructured":"Gerberick, G.F., Vassallo, J.D., Bailey, R.E., Chaney, J.G., Morrall, S.W. and Lepoittevin, J.P. (2004): Development of a peptide reactivity assay for screening contact allergens. Toxicol. Sci., <b>81<\/b>, 332-343.","DOI":"10.1093\/toxsci\/kfh213"},{"key":"4","doi-asserted-by":"crossref","unstructured":"Kimura, Y., Fujimura, C., Ito, Y., Takahashi, T., Nakajima, Y., Ohmiya, Y. and Aiba, S. (2015): Optimization of the IL-8 Luc assay as an in vitro test for skin sensitization. Toxicol. In Vitro, <b>29<\/b>, 1816-1830.","DOI":"10.1016\/j.tiv.2015.07.006"},{"key":"5","doi-asserted-by":"crossref","unstructured":"Kimura, Y., Fujimura, C., Ito, Y., Takahashi, T., Terui, H. and Aiba, S. (2018): Profiling the immunotoxicity of chemicals based on <i>in vitro<\/i> evaluation by a combination of the Multi-ImmunoTox assay and the IL-8 Luc assay. Arch. Toxicol., <b>92<\/b>, 2043-2054.","DOI":"10.1007\/s00204-018-2199-7"},{"key":"6","doi-asserted-by":"crossref","unstructured":"Nakajima, Y., Kimura, T., Sugata, K., Enomoto, T., Asakawa, A., Kubota, H., Ikeda, M. and Ohmiya, Y. (2005): Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate. Biotechniques, <b>38<\/b>, 891-894.","DOI":"10.2144\/05386ST03"},{"key":"7","doi-asserted-by":"crossref","unstructured":"Natsch, A., Ryan, C.A., Foertsch, L., Emter, R., Jaworska, J., Gerberick, F. and Kern, P. (2013): A dataset on 145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation. J. Appl. Toxicol., <b>33<\/b>, 1337-1352.","DOI":"10.1002\/jat.2868"},{"key":"8","doi-asserted-by":"crossref","unstructured":"Noguchi, T., Ikeda, M., Ohmiya, Y. and Nakajima, Y. (2008): Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases. BMC Biotechnol., <b>8<\/b>, 40.","DOI":"10.1186\/1472-6750-8-40"},{"key":"9","unstructured":"OECD (2012): The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins, Part 1: Scientific Evidence. OECD Environment, Health and Safety Publications, Series on Testing and Assessment No. 168."},{"key":"10","unstructured":"OECD. (2015a): OECD Guideline for the Testing of Chemicals No. 442D: In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method. Paris, France: Organisation for Economic Cooperation and Development. Available at: http:\/\/www.oecd.org\/env\/testguidelines."},{"key":"11","unstructured":"OECD. (2015b): OECD Guideline for the Testing of Chemicals No. 442C: In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA). Paris, France: Organisation for Economic Cooperation and Development. Available at: http:\/\/www.oecd.org\/env\/testguidelines."},{"key":"12","doi-asserted-by":"crossref","unstructured":"OECD (2018): OECD Test Gudeline for the Testing of Chemicals No.442E: In vitro skin sensitisation assays addressing the key event on activation of dendritic cells on the adverse outcome pathway for skin sensitisation. https:\/\/doi.org\/10.1787\/9789264264359-en.","DOI":"10.1787\/9789264264359-en"},{"key":"13","doi-asserted-by":"crossref","unstructured":"Reisinger, K., Hoffmann, S., Al\u00e9p\u00e9e, N., Ashikaga, T., Barroso, J., Elcombe, C., Gellatly, N., Galbiati, V., Gibbs, S., Groux, H., Hibatallah, J., Keller, D., Kern, P., Klaric, M., Kolle, S., Kuehnl, J., Lambrechts, N., Lindstedt, M., Millet, M., Martinozzi-Teissier, S., Natsch, A., Petersohn, D., Pike, I., Sakaguchi, H., Schepky, A., Tailhardat, M., Templier, M., van Vliet, E. and Maxwell, G. (2015): Systematic evaluation of non-animal test methods for skin sensitisation safety assessment. Toxicol. In Vitro, <b>29<\/b>, 259-270.","DOI":"10.1016\/j.tiv.2014.10.018"},{"key":"14","doi-asserted-by":"crossref","unstructured":"Roberts, D.W. and Patlewicz, G. (2018): Non-animal assessment of skin sensitization hazard: is an integrated testing strategy needed, and if so what should be integrated? J. Appl. Toxicol., <b>38<\/b>, 41-50.","DOI":"10.1002\/jat.3479"},{"key":"15","doi-asserted-by":"crossref","unstructured":"Smith Pease, C.K., Basketter, D.A. and Patlewicz, G.Y. (2003): Contact allergy: the role of skin chemistry and metabolism. Clin. Exp. Dermatol., <b>28<\/b>, 177-183.","DOI":"10.1046\/j.1365-2230.2003.01239.x"},{"key":"16","doi-asserted-by":"crossref","unstructured":"Takahashi, T., Kimura, Y., Saito, R., Nakajima, Y., Ohmiya, Y., Yamasaki, K. and Aiba, S. (2011): An <i>in vitro<\/i> test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8. Toxicol. Sci., <b>124<\/b>, 359-369.","DOI":"10.1093\/toxsci\/kfr237"},{"key":"17","doi-asserted-by":"crossref","unstructured":"Takenouchi, O., Fukui, S., Okamoto, K., Kurotani, S., Imai, N., Fujishiro, M., Kyotani, D., Kato, Y., Kasahara, T., Fujita, M., Toyoda, A., Sekiya, D., Watanabe, S., Seto, H., Hirota, M., Ashikaga, T. and Miyazawa, M. (2015): Test battery with the human cell line activation test, direct peptide reactivity assay and DEREK based on a 139 chemical data set for predicting skin sensitizing potential and potency of chemicals. J. Appl. Toxicol., <b>35<\/b>, 1318-1332.","DOI":"10.1002\/jat.3127"}],"container-title":["The Journal of Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/43\/12\/43_741\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T23:13:48Z","timestamp":1544224428000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/43\/12\/43_741\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":17,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.2131\/jts.43.741","relation":{},"ISSN":["0388-1350","1880-3989"],"issn-type":[{"value":"0388-1350","type":"print"},{"value":"1880-3989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}