{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:30:25Z","timestamp":1790505025604,"version":"4.1.0"},"reference-count":41,"publisher":"Japanese Society of Toxicology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Toxicol. Sci."],"published-print":{"date-parts":[[2019]]},"DOI":"10.2131\/jts.44.213","type":"journal-article","created":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T17:41:06Z","timestamp":1551894066000},"page":"213-224","source":"Crossref","is-referenced-by-count":12,"title":["Some non-sensitizers upregulate CD54 expression by activation of the NLRP3 inflammasome in THP-1 cells"],"prefix":"10.2131","volume":"44","author":[{"given":"Takafumi","family":"Mitachi","sequence":"first","affiliation":[{"name":"Department of Chemical and Energy Engineering, Yokohama National University"},{"name":"Corporate Research Center, Daicel Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Kouzui","sequence":"additional","affiliation":[{"name":"Department of Chemical and Energy Engineering, Yokohama National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Department of Chemical and Energy Engineering, Yokohama National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Corporate Research Center, Daicel Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Ogata","sequence":"additional","affiliation":[{"name":"Department of Environment and Information Science, Yokohama National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Kojima","sequence":"additional","affiliation":[{"name":"Division of Risk Assessment, National Institute of Health Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Itagaki","sequence":"additional","affiliation":[{"name":"Department of Chemical and Energy Engineering, Yokohama National University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"898","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Ade, N., Leon, F., Pallardy, M., Peiffer, J.L., Kerdine-Romer, S., Tissier, M.H., Bonnet, P.A., Fabre, I. and Ourlin, J.C. (2009): HMOX1 and NQO1 genes are upregulated in response to contact sensitizers in dendritic cells and THP-1 cell line: role of the Keap1\/Nrf2 pathway. Toxicol. Sci., <b>107<\/b>, 451-460.","DOI":"10.1093\/toxsci\/kfn243"},{"key":"2","doi-asserted-by":"crossref","unstructured":"Aiba, S., Terunuma, A., Manome, H. and Tagami, H. (1997): Dendritic cells differently respond to haptens and irritants by their production of cytokines and expression of co-stimulatory molecules. Eur. J. Immunol., <b>27<\/b>, 3031-3038.","DOI":"10.1002\/eji.1830271141"},{"key":"3","doi-asserted-by":"crossref","unstructured":"Aiba, S., Manome, H., Nakagawa, S., Mollah, Z.U., Mizuashi, M., Ohtani, T., Yoshino, Y. and Tagami, H. (2003): p38 Mitogen-activated protein kinase and extracellular signal-regulated kinases play distinct roles in the activation of dendritic cells by two representative haptens, NiCl2 and 2,4-dinitrochlorobenzene. J. Invest. Dermatol., <b>120<\/b>, 390-399.","DOI":"10.1046\/j.1523-1747.2003.12065.x"},{"key":"4","doi-asserted-by":"crossref","unstructured":"Arn\u00e9r, E.S., Bj\u00f6rnstedt, M. and Holmgren, A. (1995): 1-Chloro-2,4-dinitrobenzene is an irreversible inhibitor of human thioredoxin reductase. Loss of thioredoxin disulfide reductase activity is accompanied by a large increase in NADPH oxidase activity. J. Biol. Chem., <b>270<\/b>, 3479-3482.","DOI":"10.1074\/jbc.270.8.3479"},{"key":"5","doi-asserted-by":"crossref","unstructured":"Arn\u00e9r, E.S. (1999): Superoxide production by dinitrophenyl-derivatized thioredoxin reductase--a model for the mechanism and correlation to immunostimulation by dinitrohalobenzenes. Biofactors, <b>10<\/b>, 219-226.","DOI":"10.1002\/biof.5520100220"},{"key":"6","doi-asserted-by":"crossref","unstructured":"Arrighi, J.F., Rebsamen, M., Rousset, F., Kindler, V. and Hauser, C. (2001): A critical role for p38 mitogen-activated protein kinase in the maturation of human blood-derived dendritic cells induced by lipopolysaccharide, TNF-\u03b1, and contact sensitizers. J. Immunol., <b>166<\/b>, 3837-3845.","DOI":"10.4049\/jimmunol.166.6.3837"},{"key":"7","doi-asserted-by":"crossref","unstructured":"Ashikaga, T., Hoya, M., Itagaki, H., Katsumura, Y. and Aiba, S. (2002): Evaluation of CD86 expression and MHC class II molecule internalization in THP-1 human monocyte cells as predictive endpoints for contact sensitizers. Toxicol. In Vitro, <b>16<\/b>, 711-716.","DOI":"10.1016\/S0887-2333(02)00060-7"},{"key":"8","doi-asserted-by":"crossref","unstructured":"Ashikaga, T., Sakaguchi, H., Sono, S., Kosaka, N., Ishikawa, M., Nukada, Y., Miyazawa, M., Ito, Y., Nishiyama, N. and Itagaki, H. (2010): A comparative evaluation of <i>in vitro<\/i> skin sensitisation tests: the human cell-line activation test (h-CLAT) versus the local lymph node assay (LLNA). Altern. Lab. Anim., <b>38<\/b>, 275-284.","DOI":"10.1177\/026119291003800403"},{"key":"9","doi-asserted-by":"crossref","unstructured":"Ashikaga, T., Yoshida, Y., Hirota, M., Yoneyama, K., Itagaki, H., Sakaguchi, H., Miyazawa, M., Ito, Y., Suzuki, H. and Toyoda, H. (2006): Development of an <i>in vitro<\/i> skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol. Toxicol. In Vitro, <b>20<\/b>, 767-773.","DOI":"10.1016\/j.tiv.2005.10.012"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Chen, C.C., Chen, J.J. and Chou, C.Y. (2000): Protein kinase c\u03b1 but not p44\/42 mitogen-activated protein kinase, p38, or c-Jun NH(2)-terminal kinase is required for intercellular adhesion molecule-1 expression mediated by interleukin-1\u03b2: involvement of sequential activation of tyrosine kinase, nuclear factor-\u03baB-inducing kinase, and IkappaB kinase 2. Mol. Pharmacol., <b>58<\/b>, 1479-1489.","DOI":"10.1124\/mol.58.6.1479"},{"key":"11","doi-asserted-by":"crossref","unstructured":"Coutant, K.D., de Fraissinette, A.B., Cordier, A. and Ulrich, P. (1999): Modulation of the activity of human monocyte-derived dendritic cells by chemical haptens, a metal allergen, and a staphylococcal superantigen. Toxicol. Sci., <b>52<\/b>, 189-198.","DOI":"10.1093\/toxsci\/52.2.189"},{"key":"12","doi-asserted-by":"publisher","unstructured":"Cumberbatch, M., Dearman, R.J. and Kimber, I. (1996): Constitutive and inducible expression of interleukin-6 by Langerhans cells and lymph node dendritic cells. Immunology, <b>87<\/b>, 513-518.","DOI":"10.1046\/j.1365-2567.1996.504577.x"},{"key":"13","doi-asserted-by":"crossref","unstructured":"Dostert, C., P\u00e9trilli, V., Van Bruggen, R., Steele, C., Mossman, B.T. and Tschopp, J. (2008): Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science, <b>320<\/b>, 674-677.","DOI":"10.1126\/science.1156995"},{"key":"14","unstructured":"EC EURL-ECVAM. (2012): human Cell Line Activation Test (h-CLAT) Validation Study Report."},{"key":"15","doi-asserted-by":"crossref","unstructured":"Enk, A.H. and Katz, S.I. (1992): Early molecular events in the induction phase of contact sensitivity. Proc. Natl. Acad. Sci. USA, <b>89<\/b>, 1398-1402.","DOI":"10.1073\/pnas.89.4.1398"},{"key":"16","doi-asserted-by":"crossref","unstructured":"Fernandes-Alnemri, T., Wu, J., Yu, J.W., Datta, P., Miller, B., Jankowski, W., Rosenberg, S., Zhang, J. and Alnemri, E.S. (2007): The pyroptosome: a supramolecular assembly of ASC dimers mediating inflammatory cell death via caspase-1 activation. Cell Death Differ., <b>14<\/b>, 1590-1604.","DOI":"10.1038\/sj.cdd.4402194"},{"key":"17","first-page":"157","year":"2005","unstructured":"Gerberick, G.F., Ryan, C.A., Kern, P.S., Schlatter, H., Dearman, R.J., Kimber, I., Patlewicz, G.Y. and Basketter, D.A. (2005): Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods. Dermatitis, <b>16<\/b>, 157-202.","journal-title":"Dermatitis","ISSN":"https:\/\/id.crossref.org\/issn\/1710-3568","issn-type":"print"},{"key":"18","doi-asserted-by":"crossref","unstructured":"Kaplan, D.H., Igy\u00e1rt\u00f3, B.Z. and Gaspari, A.A. (2012): Early immune events in the induction of allergic contact dermatitis. Nat. Rev. Immunol., <b>12<\/b>, 114-124.","DOI":"10.1038\/nri3150"},{"key":"19","doi-asserted-by":"crossref","unstructured":"Kaspar, J.W., Niture, S.K. and Jaiswal, A.K. (2009): Nrf2:INrf2 (Keap1) signaling in oxidative stress. Free Radic. Biol. Med., <b>47<\/b>, 1304-1309.","DOI":"10.1016\/j.freeradbiomed.2009.07.035"},{"key":"20","doi-asserted-by":"crossref","unstructured":"Martin, S.F., Dudda, J.C., Bachtanian, E., Lembo, A., Liller, S., D\u00fcrr, C., Heimesaat, M.M., Bereswill, S., Fejer, G., Vassileva, R., Jakob, T., Freudenberg, N., Termeer, C.C., Johner, C., Galanos, C. and Freudenberg, M.A. (2008): Toll-like receptor and IL-12 signaling control susceptibility to contact hypersensitivity. J. Exp. Med., <b>205<\/b>, 2151-2162.","DOI":"10.1084\/jem.20070509"},{"key":"21","doi-asserted-by":"crossref","unstructured":"Mitachi, T., Mezaki, M., Yamashita, K. and Itagaki, H. (2018): Acidic conditions induce the suppression of CD86 and CD54 expression in THP-1 cells. J. Toxicol. Sci., <b>43<\/b>, 299-309.","DOI":"10.2131\/jts.43.299"},{"key":"22","doi-asserted-by":"crossref","unstructured":"Narita, K., Ishii, Y., Vo, P.T., Nakagawa, F., Ogata, S., Yamashita, K., Kojima, H. and Itagaki, H. (2018): Improvement of human cell line activation test (h-CLAT) using short-time exposure methods for prevention of false-negative results. J. Toxicol. Sci., <b>43<\/b>, 229-240.","DOI":"10.2131\/jts.43.229"},{"key":"23","doi-asserted-by":"crossref","unstructured":"Natsch, A., Ryan, C.A., Foertsch, L., Emter, R., Jaworska, J., Gerberick, F. and Kern, P. (2013): A dataset on 145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation. J. Appl. Toxicol., <b>33<\/b>, 1337-1352.","DOI":"10.1002\/jat.2868"},{"key":"24","doi-asserted-by":"crossref","unstructured":"Nordberg, J., Zhong, L., Holmgren, A. and Arn\u00e9r, E.S. (1998): Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue. J. Biol. Chem., <b>273<\/b>, 10835-10842.","DOI":"10.1074\/jbc.273.18.10835"},{"key":"25","doi-asserted-by":"crossref","unstructured":"Nukada, Y., Ito, Y., Miyazawa, M., Sakaguchi, H. and Nishiyama, N. (2011): The relationship between CD86 and CD54 protein expression and cytotoxicity following stimulation with contact allergen in THP-1 cells. J. Toxicol. Sci., <b>36<\/b>, 313-324.","DOI":"10.2131\/jts.36.313"},{"key":"26","doi-asserted-by":"crossref","unstructured":"OECD. (2014): The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins. OECD Series on Testing and Assessment, No. 168.","DOI":"10.1787\/9789264221444-en"},{"key":"27","unstructured":"OECD. (2018): OECD Guidelines for the Testing of Chemicals, Section 4 \/ Test No. 442E: In vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome pathway for Skin Sensitisation, adopted June 2018. Paris, France: Organisation for Economic Cooperation and Development."},{"key":"28","doi-asserted-by":"crossref","unstructured":"Peiser, M., Tralau, T., Heidler, J., Api, A.M., Arts, J.H., Basketter, D.A., English, J., Diepgen, T.L., Fuhlbrigge, R.C., Gaspari, A.A., Johansen, J.D., Karlberg, A.T., Kimber, I., Lepoittevin, J.P., Liebsch, M., Maibach, H.I., Martin, S.F., Merk, H.F., Platzek, T., Rustemeyer, T., Schnuch, A., Vandebriel, R.J., White, I.R. and Luch, A. (2012): Allergic contact dermatitis: epidemiology, molecular mechanisms, <i>in vitro<\/i> methods and regulatory aspects. Current knowledge assembled at an international workshop at BfR, Germany. Cell. Mol. Life Sci., <b>69<\/b>, 763-781.","DOI":"10.1007\/s00018-011-0846-8"},{"key":"29","doi-asserted-by":"crossref","unstructured":"P\u00e9trilli, V., Papin, S., Dostert, C., Mayor, A., Martinon, F. and Tschopp, J. (2007): Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration. Cell Death Differ., <b>14<\/b>, 1583-1589.","DOI":"10.1038\/sj.cdd.4402195"},{"key":"30","doi-asserted-by":"crossref","unstructured":"Rajam\u00e4ki, K., Nordstr\u00f6m, T., Nurmi, K., \u00c5kerman, K.E., Kovanen, P.T., \u00d6\u00f6rni, K. and Eklund, K.K. (2013): Extracellular acidosis is a novel danger signal alerting innate immunity via the NLRP3 inflammasome. J. Biol. Chem., <b>288<\/b>, 13410-13419.","DOI":"10.1074\/jbc.M112.426254"},{"key":"31","doi-asserted-by":"crossref","unstructured":"Roebuck, K.A. and Finnegan, A. (1999): Regulation of intercellular adhesion molecule-1 (CD54) gene expression. J. Leukoc. Biol., <b>66<\/b>, 876-888.","DOI":"10.1002\/jlb.66.6.876"},{"key":"32","doi-asserted-by":"crossref","unstructured":"Saito, K., Miyazawa, M., Nukada, Y., Sakaguchi, H. and Nishiyama, K. (2013): Development of an in vitro skin sensitization test based on ROS production in THP-1 cells. Toxicol. In Vitro, <b>2<\/b>, 857-863.","DOI":"10.1016\/j.tiv.2012.12.025"},{"key":"33","doi-asserted-by":"crossref","unstructured":"Sakaguchi, H., Ashikaga, T., Miyazawa, M., Yoshida, Y., Ito, Y., Yoneyama, K., Hirota, M., Itagaki, H., Toyoda, H. and Suzuki, H. (2006): Development of an <i>in vitro<\/i> skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT. Toxicol. In Vitro, <b>20<\/b>, 774-784.","DOI":"10.1016\/j.tiv.2005.10.014"},{"key":"34","doi-asserted-by":"crossref","unstructured":"Schmidt, M., Raghavan, B., M\u00fcller, V., Vogl, T., Fejer, G., Tchaptchet, S., Keck, S., Kalis, C., Nielsen, P.J., Galanos, C., Roth, J., Skerra, A., Martin, S.F., Freudenberg, M.A. and Goebeler, M. (2010): Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nat. Immunol., <b>11<\/b>, 814-819.","DOI":"10.1038\/ni.1919"},{"key":"35","doi-asserted-by":"crossref","unstructured":"Takenouchi, O., Miyazawa, M., Saito, K., Ashikaga, T. and Sakaguchi, H. (2013): Predictive performance of the human Cell Line Activation Test (h-CLAT) for lipophilic chemicals with high octanol-water partition coefficients. J. Toxicol. Sci., <b>38<\/b>, 599-609.","DOI":"10.2131\/jts.38.599"},{"key":"36","first-page":"585","year":"2009","unstructured":"Trompette, A., Divanovic, S., Visintin, A., Blanchard, C., Hegde, R.S., Madan, R., Thorne, P.S., Wills-Karp, M., Gioannini, T.L., Weiss, J.P. and Karp, C.L. (2009): Allergenicity resulting from functional mimicry of a Toll-like receptor complex protein. Nature, <b>457<\/b>, 585-588.","journal-title":"Nature Cell Biology","ISSN":"https:\/\/id.crossref.org\/issn\/0028-0836","issn-type":"print"},{"key":"37","doi-asserted-by":"crossref","unstructured":"Tsukumo, H., Matsunari, N., Yamashita, K., Kojima, H. and Itagaki, H. (2018): Lipopolysaccharide interferes with the use of the human Cell Line Activation Test to determine the allergic potential of proteins. J. Pharmacol. Toxicol. Methods, <b>92<\/b>, 34-42.","DOI":"10.1016\/j.vascn.2018.02.003"},{"key":"38","doi-asserted-by":"crossref","unstructured":"Watanabe, H., Gaide, O., P\u00e9trilli, V., Martinon, F., Contassot, E., Roques, S., Kummer, J.A., Tschopp, J. and French, L.E. (2007): Activation of the IL-1\u03b2-processing inflammasome is involved in contact hypersensitivity. J. Invest. Dermatol., <b>127<\/b>, 1956-1963.","DOI":"10.1038\/sj.jid.5700819"},{"key":"39","doi-asserted-by":"crossref","unstructured":"Weber, F.C., Esser, P.R., M\u00fcller, T., Ganesan, J., Pellegatti, P., Simon, M.M., Zeiser, R., Idzko, M., Jakob, T. and Martin, S.F. (2010): Lack of the purinergic receptor P2X(7) results in resistance to contact hypersensitivity. J. Exp. Med., <b>207<\/b>, 2609-2619.","DOI":"10.1084\/jem.20092489"},{"key":"40","doi-asserted-by":"crossref","unstructured":"Xia, X., Shi, Q., Song, X., Fu, J., Liu, Z., Wang, Y., Wang, Y., Su, C., Song, E. and Song, Y. (2016): Tetrachlorobenzoquinone Stimulates NLRP3 Inflammasome-Mediated Post-Translational Activation and Secretion of IL-1\u03b2 in the HUVEC Endothelial Cell Line. Chem. Res. Toxicol., <b>29<\/b>, 421-429.","DOI":"10.1021\/acs.chemrestox.6b00021"},{"key":"41","doi-asserted-by":"crossref","unstructured":"Yoshida, Y., Sakaguchi, H., Ito, Y., Okuda, M. and Suzuki, H. (2003): Evaluation of the skin sensitization potential of chemicals using expression of co-stimulatory molecules, CD54 and CD86, on the naive THP-1 cell line. Toxicol. In Vitro, <b>17<\/b>, 221-228.","DOI":"10.1016\/S0887-2333(03)00006-7"}],"container-title":["The Journal of Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/44\/3\/44_213\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T00:52:42Z","timestamp":1574211162000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/44\/3\/44_213\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.2131\/jts.44.213","relation":{},"ISSN":["0388-1350","1880-3989"],"issn-type":[{"value":"0388-1350","type":"print"},{"value":"1880-3989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}