{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T06:14:46Z","timestamp":1775888086887,"version":"3.50.1"},"reference-count":25,"publisher":"Japanese Society of Toxicology","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Toxicol. Sci."],"published-print":{"date-parts":[[2020]]},"DOI":"10.2131\/jts.45.769","type":"journal-article","created":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T22:14:05Z","timestamp":1606774445000},"page":"769-782","source":"Crossref","is-referenced-by-count":4,"title":["LC\/MS analysis of three-dimensional model cells exposed to cigarette smoke or aerosol from a novel tobacco vapor product"],"prefix":"10.2131","volume":"45","author":[{"given":"Yuichiro","family":"Takanami","sequence":"first","affiliation":[{"name":"Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobumasa","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeaki","family":"Ito","sequence":"additional","affiliation":[{"name":"Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"898","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Baxter, A., Thain, S., Banerjee, A., Haswell, L., Parmar, A., Phillips, G. and Minet, E. (2015): Targeted omics analyses, and metabolic enzyme activity assays demonstrate maintenance of key mucociliary characteristics in long term cultures of reconstituted human airway epithelia. Toxicol. In Vitro, <b>29<\/b>, 864-875.","DOI":"10.1016\/j.tiv.2015.03.004"},{"key":"2","doi-asserted-by":"crossref","unstructured":"Benowitz, N.L., Hukkanen, J. and Jacob, P. 3rd. (2009): Nicotine chemistry, metabolism, kinetics and biomarkers. Handb. Exp. Pharmacol., <b>192<\/b>, 29-60.","DOI":"10.1007\/978-3-540-69248-5_2"},{"key":"3","doi-asserted-by":"crossref","unstructured":"Bentley, M.C., Almstetter, M., Arndt, D., Knorr, A., Martin, E., Pospisil, P. and Maeder, S. (2020): Comprehensive chemical characterization of the aerosol generated by a heated tobacco product by untargeted screening. Anal. Bioanal. Chem., <b>412<\/b>, 2675-2685.","DOI":"10.1007\/s00216-020-02502-1"},{"key":"4","unstructured":"CORESTA (2015): Routine analytical machine for e-cigarette aerosol generation and collection \u2013 Definitions and standard conditions. CORESTA recommended method N\u00ba 81."},{"key":"5","doi-asserted-by":"crossref","unstructured":"Ishikawa, S. and Ito, S. (2017): Repeated whole cigarette smoke exposure alters cell differentiation and augments secretion of inflammatory mediators in air-liquid interface three-dimensional co-culture model of human bronchial tissue. Toxicol. In Vitro, <b>38<\/b>, 170-178.","DOI":"10.1016\/j.tiv.2016.09.004"},{"key":"6","doi-asserted-by":"crossref","unstructured":"Ishikawa, S., Matsumura, K., Kitamura, N., Takanami, Y. and Ito, S. (2019): Multi-omics analysis: repeated exposure of a 3D bronchial tissue culture to whole-cigarette smoke. Toxicol. In Vitro, <b>54<\/b>, 251-262.","DOI":"10.1016\/j.tiv.2018.10.001"},{"key":"7","unstructured":"ISO 20778:2018 (2018): Cigarettes -- Routine analytical cigarette smoking machine -- Definitions and standard conditions with an intense smoking regime. International Organization for Standardization, Geneva."},{"key":"8","unstructured":"ISO 3402:1999 (1999): Tobacco and tobacco products -- Atmosphere for conditioning and testing. International Organization for Standardization, Geneva."},{"key":"9","doi-asserted-by":"crossref","unstructured":"Ito, S., Matsumura, K., Ishimori, K. and Ishikawa, S. (2020): <i>In vitro<\/i> long-term repeated exposure and exposure switching of a novel tobacco vapor product in a human organotypic culture of bronchial epithelial cells. J. Appl. Toxicol., <b>40<\/b>, 1248-1258.","DOI":"10.1002\/jat.3982"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Knorr, A., Almstetter, M., Martin, E., Castellon, A., Pospisil, P. and Bentley, M.C. (2019): Performance evaluation of a nontargeted platform using two-dimensional gas chromatography time-of-flight mass spectrometry integrating computer-assisted structure identification and automated semiquantification for the comprehensive chemical characterization of a complex matrix. Anal. Chem., <b>91<\/b>, 9129-9137.","DOI":"10.1021\/acs.analchem.9b01659"},{"key":"11","first-page":"92","year":"2019","unstructured":"Mathon, C., Bovard, D., Dutertre, Q., Sendyk, S., Bentley, M., Hoeng, J. and Knorr, A. (2019): Impact of sample preparation upon intracellular metabolite measurements in 3D cell culture systems. Metabolomics, <b>15<\/b>, 92.","journal-title":"Metabolomics","ISSN":"https:\/\/id.crossref.org\/issn\/1573-3882","issn-type":"print"},{"key":"12","doi-asserted-by":"crossref","unstructured":"Munakata, S., Ishimori, K., Kitamura, N., Ishikawa, S., Takanami, Y. and Ito, S. (2018): Oxidative stress responses in human bronchial epithelial cells exposed to cigarette smoke and vapor from tobacco- and nicotine-containing products. Regul. Toxicol. Pharmacol., <b>99<\/b>, 122-128.","DOI":"10.1016\/j.yrtph.2018.09.009"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-395"},{"key":"14","unstructured":"R Core Team. (2016): R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria."},{"key":"15","unstructured":"Rodgman, A. and Perfetti, T.A. (2013): The chemical components of tobacco and tobacco smoke, 2nd edition. CRC press, Boca Raton."},{"key":"16","doi-asserted-by":"crossref","unstructured":"Saigusa, D., Okamura, Y., Motoike, I.N., Katoh, Y., Kurosawa, Y., Saijyo, R., Koshiba, S., Yasuda, J., Motohashi, H., Sugawara, J., Tanabe, O., Kinoshita, K. and Yamamoto, M. (2016): Establishment of protocols for global metabolomics by LC-MS for biomarker discovery. PLoS One, <b>11<\/b>, e0160555.","DOI":"10.1371\/journal.pone.0160555"},{"key":"17","doi-asserted-by":"crossref","unstructured":"Schymanski, E.L., Jeon, J., Gulde, R., Fenner, K., Ruff, M., Singer, H.P. and Hollender, J. (2014): Identifying small molecules via high resolution mass spectrometry: communicating confidence. Environ. Sci. Technol., <b>48<\/b>, 2097-2098.","DOI":"10.1021\/es5002105"},{"key":"18","first-page":"1050","year":"2014","unstructured":"Southam, A.D., Lange, A., Al-Salhi, R., Hill, E.M., Tyler, C.R. and Viant, M.R. (2014): Distinguishing between the metabolome and xenobiotic exposome in environmental field samples analysed by direct-infusion mass spectrometry based metabolomics and lipidomics. Metabolomics, <b>10<\/b>, 1050-1058.","journal-title":"Metabolomics","ISSN":"https:\/\/id.crossref.org\/issn\/1573-3882","issn-type":"print"},{"key":"19","doi-asserted-by":"crossref","unstructured":"Takahashi, Y., Kanemaru, Y., Fukushima, T., Eguchi, K., Yoshida, S., Miller-Holt, J. and Jones, I. (2018): Chemical analysis and <i>in vitro<\/i> toxicological evaluation of aerosol from a novel tobacco vapor product: A comparison with cigarette smoke. Regul. Toxicol. Pharmacol., <b>92<\/b>, 94-103.","DOI":"10.1016\/j.yrtph.2017.11.009"},{"key":"20","doi-asserted-by":"crossref","unstructured":"Vorrink, S.U., Ullah, S., Schmidt, S., Nandania, J., Velagapudi, V., Beck, O., Ingelman-Sundberg, M. and Lauschke, V.M. (2017): Endogenous and xenobiotic metabolic stability of primary human hepatocytes in long-term 3D spheroid cultures revealed by a combination of targeted and untargeted metabolomics. FASEB J., <b>31<\/b>, 2696-2708.","DOI":"10.1096\/fj.201601375R"},{"key":"21","unstructured":"Wishart, D.S., Feunang, Y.D., Marcu, A., Guo, A.C., Liang, K., V\u00e1zquez-Fresno, R., Sajed, T., Johnson, D., Li, C., Karu, N., Sayeeda, Z., Lo, E., Assempour, N., Berjanskii, M., Singhal, S., Arndt, D., Liang, Y., Badran, H., Grant, J., Serra-Cayuela, A., Liu, Y., Mandal, R., Neveu, V., Pon, A., Knox, C., Wilson, M., Manach, C. and Scalbert, A. (2018): HMDB 4.0: the human metabolome database for 2018. Nucleic Acids Res., <b>46<\/b> (D1), D608-D617."},{"key":"22","doi-asserted-by":"crossref","unstructured":"Yuki, D., Takeshige, Y., Nakaya, K. and Futamura, Y. (2018): Assessment of the exposure to harmful and potentially harmful constituents in healthy Japanese smokers using a novel tobacco vapor product compared with conventional cigarettes and smoking abstinence. Regul. Toxicol. Pharmacol., <b>96<\/b>, 127-134.","DOI":"10.1016\/j.yrtph.2018.05.001"},{"key":"23","doi-asserted-by":"crossref","unstructured":"Zanetti, F., Titz, B., Sewer, A., Lo Sasso, G., Scotti, E., Schlage, W.K., Mathis, C., Leroy, P., Majeed, S., Torres, L.O., Keppler, B.R., Elamin, A., Trivedi, K., Guedj, E., Martin, F., Frentzel, S., Ivanov, N.V., Peitsch, M.C. and Hoeng, J. (2017): Comparative systems toxicology analysis of cigarette smoke and aerosol from a candidate modified risk tobacco product in organotypic human gingival epithelial cultures: A 3-day repeated exposure study. Food Chem. Toxicol., <b>101<\/b>, 15-35.","DOI":"10.1016\/j.fct.2016.12.027"},{"key":"24","doi-asserted-by":"crossref","unstructured":"Zhang, L., Ashley, D.L. and Watson, C.H. (2011): Quantitative analysis of six heterocyclic aromatic amines in mainstream cigarette smoke condensate using isotope dilution liquid chromatography-electrospray ionization tandem mass spectrometry. Nicotine Tob. Res., <b>13<\/b>, 120-126.","DOI":"10.1093\/ntr\/ntq219"},{"key":"25","doi-asserted-by":"crossref","unstructured":"Zhang, X., Wang, R., Zhang, L., Ruan, Y., Wang, W., Ji, H., Lin, F. and Liu, J. (2018): Simultaneous determination of tobacco minor alkaloids and tobacco-specific nitrosamines in mainstream smoke by dispersive solid-phase extraction coupled with ultra-performance liquid chromatography\/tandem orbitrap mass spectrometry. Rapid Commun. Mass Spectrom., <b>32<\/b>, 1791-1798.","DOI":"10.1002\/rcm.8222"}],"container-title":["The Journal of Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/45\/12\/45_769\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:39:28Z","timestamp":1607139568000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jts\/45\/12\/45_769\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":25,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.2131\/jts.45.769","relation":{},"ISSN":["0388-1350","1880-3989"],"issn-type":[{"value":"0388-1350","type":"print"},{"value":"1880-3989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]}}}