{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T19:58:15Z","timestamp":1775332695499,"version":"3.50.1"},"reference-count":0,"publisher":"Universiti Sains Malaysia","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MJMS"],"abstract":"<jats:p>Background: The present study aimed to understand the characterisation of human hippocampal astrocyte following hypoxia exposure. Based on the preliminary screening, 15 min was chosen as the time point and the cells were exposed to different oxygen percentages.   Methods: The Trypan blue viability assay used to examine cell death. Immunofluorescence assay, glial fibrillary acidic protein (GFAP) was used to portray the morphology of astrocytes. The hypoxia-inducible factor 1 (HIF-1) staining was performed to confirm hypoxia induced cell death and there was a dramatic expression of HIF-1\u03b1 displayed in exposed astrocyte cells compared to the control. In molecular level, genes were chosen, such as glyceraldehyde 3-phosphate dehydrogenase (GAPDH), GFAP, HIF-1\u03b1 and B-cell lymphoma 2 (Bcl-2) and ran the reverse transcription-polymerase chain reaction (RT-PCR).   Results: Microscope revealed a filamentous and clear nucleus appearance in a control whereas the rupture nuclei with no rigid structure of the cell were found in the 3% oxygen. The control and hypoxia cells were also stained with the annexin V-fluorescein isothiocyanate (annexin V-FITC). Fluorescence microscope reveals astrocyte cells after hypoxia showed higher expression of nuclei but not in control. Merging PI and FITC showed the differences of nuclei expression between the control and hypoxia. In the molecular analysis, there were significant changes of GFAP, HIF-1\u03b1 and Bcl-2 in hypoxia exposed cells when compared to the control group.   Conclusion: Cells that were exposed to hypoxia (3% oxygen for 15 min) clearly showed damage. General view of human hippocampal astrocyte genomic response to hypoxia was obtained.<\/jats:p>","DOI":"10.21315\/mjms2023.30.1.8","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T04:44:16Z","timestamp":1677645856000},"page":"92-106","source":"Crossref","is-referenced-by-count":1,"title":["Characterisation of Primary Human Hippocampal Astrocyte Cell Culture Following Exposure to Hypoxia"],"prefix":"10.21315","volume":"30","author":[{"name":"Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurul Atikah","family":"Nor Nazli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Brain and Behaviour Cluster, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangu","family":"Muthuraju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Brain and Behaviour Cluster, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farizan","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Brain and Behaviour Cluster, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul Aziz","family":"Mohamed Yusoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Brain and Behaviour Cluster, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasnan","family":"Jaafar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaharum","family":"Shamsuddin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Biomedicine, School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jafri Malin","family":"Abdullah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Brain and Behaviour Cluster, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"8963","published-online":{"date-parts":[[2023,2,28]]},"container-title":["Malaysian Journal of Medical Sciences"],"original-title":[],"deposited":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T04:44:31Z","timestamp":1677645871000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.mjms.usm.my\/MJMS30012023\/MJMS30012023_08.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,28]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,2,28]]}},"URL":"https:\/\/doi.org\/10.21315\/mjms2023.30.1.8","relation":{},"ISSN":["1394-195X","2180-4303"],"issn-type":[{"value":"1394-195X","type":"print"},{"value":"2180-4303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,28]]}}}