{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:18:22Z","timestamp":1761895102169,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"USEPA"},{"name":"Joint Bioremediation Program"},{"name":"National Institute of Environmental Health Sciences Superfund Basic Research Program"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Env Quality"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study investigated the interaction between microbial growth and cell elution, and their influence on resultant microbial distribution between the aqueous and solid phases during solute transport in a sandy, low\u2010organic\u2010carbon\u2010content porous medium. Miscible displacement experiments were conducted with salicylate as the model compound, and with different initial conditions (e.g., substrate concentrations and cell densities) to attain various degrees of microbial growth. For each experiment, salicylate and dissolved oxygen concentrations as well as cell densities were monitored in the column effluent. Cell densities were also measured in the porous medium at the beginning and end of each experiment. Total microbial growth was determined in two ways, one based on a cell mass balance for the system and the other based on total amount of salicylate degraded. For conditions yielding a considerable amount of microbial growth, the majority of the biomass was associated with the aqueous phase (68\u201390%). Conversely, under minimal\u2010growth conditions, most cells (approximately 60\u201370%) were attached to particle surfaces. Significant cell elution was observed for most conditions, the rate of which increased in the presence of the substrate. The results suggest that the increase in aqueous\u2010phase cells observed for the experiments exhibiting the greatest growth is associated with the production of new cells, and that under appropriate conditions aqueous\u2010phase biomass can contribute significantly to contaminant biodegradation.<\/jats:p>","DOI":"10.2134\/jeq2002.1824","type":"journal-article","created":{"date-parts":[[2010,6,14]],"date-time":"2010-06-14T03:07:02Z","timestamp":1276484822000},"page":"1824-1830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Biodegradation during Contaminant Transport in Porous Media"],"prefix":"10.1002","volume":"31","author":[{"given":"Irfan","family":"Yolcubal","sequence":"first","affiliation":[{"name":"Dep. of Hydrology and Water Resources Univ. of Arizona 429 Shantz Tucson AZ 85721"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley A.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Dep. of Soil, Water, and Environmental Science Univ. of Arizona 429 Shantz Tucson AZ 85721"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raina M.","family":"Maier","sequence":"additional","affiliation":[{"name":"Dep. of Soil, Water, and Environmental Science Univ. of Arizona 429 Shantz Tucson AZ 85721"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark L.","family":"Brusseau","sequence":"additional","affiliation":[{"name":"Dep. of Hydrology and Water Resources Univ. of Arizona 429 Shantz Tucson AZ 85721"},{"name":"Dep. of Soil, Water, and Environmental Science Univ. of Arizona 429 Shantz Tucson AZ 85721"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0169-7722(89)90030-2","article-title":"Microbial adaption to degradation of hydrocarbons in polluted and unpolluted groundwater","volume":"4","author":"Aamand J.","year":"1984","journal-title":"J. Contam. Hydrol."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01490459409377993"},{"volume-title":"Cleanup of VOCs in non\u2010arid soils\u2014The Savannah River Integrated Demonstration","year":"1993","author":"Department of Energy","key":"e_1_2_7_4_1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-6584.1992.tb01795.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-7722(92)90052-G"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.48.6.1197-1202.1984"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1029\/91WR00241","article-title":"Large\u2010scale natural gradient tracer test in sand and gravel, Cape Cod, Massachusetts. 1. Experimental design and observed tracer movement","volume":"27","author":"Le Blanc D.R.","year":"1991","journal-title":"Water Resour. Res."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1029\/WR022i013p02017"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100409900043"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1029\/96WR03851"},{"volume-title":"In\u2010situ bioremediation: When does it work?","year":"1993","author":"National Research Council","key":"e_1_2_7_12_1"},{"volume-title":"A molecular approach","year":"1990","author":"Neidhardt F.C.","key":"e_1_2_7_13_1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3473-3482.1999"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-7722(01)00112-7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1998.00472425002700060025x"},{"key":"e_1_2_7_17_1","unstructured":"Yolcubal I.2001.In\u2010situmonitoring of microbial activity and biodegradation during solute transport in porous media. Ph.D. thesis. Univ. of Arizona Tucson."}],"container-title":["Journal of Environmental Quality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2134%2Fjeq2002.1824","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.2134\/jeq2002.1824","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:50:47Z","timestamp":1760633447000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/10.2134\/jeq2002.1824"}},"subtitle":["V. The Influence of Growth and Cell Elution on Microbial Distribution"],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.2134\/jeq2002.1824"],"URL":"https:\/\/doi.org\/10.2134\/jeq2002.1824","archive":["Portico"],"relation":{},"ISSN":["0047-2425","1537-2537"],"issn-type":[{"type":"print","value":"0047-2425"},{"type":"electronic","value":"1537-2537"}],"subject":[],"published":{"date-parts":[[2002,11]]},"assertion":[{"value":"2001-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-11-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}