{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T15:57:19Z","timestamp":1767196639436,"version":"build-2238731810"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["U347"],"award-info":[{"award-number":["U347"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["U488"],"award-info":[{"award-number":["U488"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003031","name":"Agence de l'Environnement et de la Ma\u00eetrise de l'Energie","doi-asserted-by":"publisher","award":["0075012"],"award-info":[{"award-number":["0075012"]}],"id":[{"id":"10.13039\/501100003031","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Env Quality"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    As part of a project studying the interactions between farming practices, soil erosion processes, and fate of agricultural pollutants into runoff waters, we conducted a pilot study to investigate the relationship between metal contents and metallothionein\u20102A (\n                    <jats:italic>MT\u20102A<\/jats:italic>\n                    ) as a bioindicator of metal exposure. Runoff water samples were collected between May and November 1999 at the point of outlet of an elementary watershed located in the Paris basin. Selected metals (Al, As, Cd, Cr, Cu, Pb, Hg, Ni, and Zn) were analyzed using conventional techniques. In parallel, human T cells were exposed to water samples for 6 and 18 h and then cell viability and\n                    <jats:italic>MT\u20102A<\/jats:italic>\n                    gene expression were measured. Results show that among the 10 water samples tested, Al and Zn predominate (highest values = 4.9 and 2.6 \u03bc\n                    <jats:italic>M<\/jats:italic>\n                    , respectively), while other metals were below the \u03bc\n                    <jats:italic>M<\/jats:italic>\n                    level. Five out of 10 samples induced\n                    <jats:italic>MT\u20102A<\/jats:italic>\n                    gene expression (30\u201380% increase at 18 h) as compared with the control. When comparing\n                    <jats:italic>MT\u20102A<\/jats:italic>\n                    induction profile with metals contents, no obvious correlation was found, suggesting that additional components or parameters are involved. Finally, there was an apparent inverse relationship between Ca concentration and\n                    <jats:italic>MT\u20102A<\/jats:italic>\n                    gene induction. Although still preliminary, in the absence of longer monitoring, this study shows that\n                    <jats:italic>MT\u20102A<\/jats:italic>\n                    gene expression is a useful tool to complement chemical analysis in assessing metal elements in water. These combinatory approaches will be pursued and integrated in an ongoing watershed field research project.\n                  <\/jats:p>","DOI":"10.2134\/jeq2004.1490","type":"journal-article","created":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T11:07:08Z","timestamp":1374664028000},"page":"149-153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Combinatory Chemical and Biological Approaches to Investigate Metal Elements in Agricultural Runoff Water"],"prefix":"10.1002","volume":"33","author":[{"given":"Renaud","family":"Quilb\u00e9","sequence":"first","affiliation":[{"name":"Centre de Biog\u00e9ographie\u2010Ecologie FRE 2545 CNRS\/ENS\u2010LSH, 15 Parvis Ren\u00e9 Descartes 69366 Lyon cedex 07 France"},{"name":"INRS\u2013Eau, Terre &amp; Environnement Universit\u00e9 du Qu\u00e9bec 2800 rue EInstein, C.P. 7500 Sainte\u2010Foy Qu\u00e9bec G1V 4C7 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Pieri","sequence":"additional","affiliation":[{"name":"Centre de Biog\u00e9ographie\u2010Ecologie FRE 2545 CNRS\/ENS\u2010LSH, 15 Parvis Ren\u00e9 Descartes 69366 Lyon cedex 07 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanislas","family":"Wicherek","sequence":"additional","affiliation":[{"name":"Centre de Biog\u00e9ographie\u2010Ecologie FRE 2545 CNRS\/ENS\u2010LSH, 15 Parvis Ren\u00e9 Descartes 69366 Lyon cedex 07 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Dugas","sequence":"additional","affiliation":[{"name":"Vigicell, Institut Andr\u00e9 Lwoff B\u00e2t. G, 7 rue Guy Moquet 94801 Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Tasteyre","sequence":"additional","affiliation":[{"name":"Vigicell, Institut Andr\u00e9 Lwoff B\u00e2t. G, 7 rue Guy Moquet 94801 Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yol\u00e8ne","family":"Thomas","sequence":"additional","affiliation":[{"name":"Centre de Biog\u00e9ographie\u2010Ecologie FRE 2545 CNRS\/ENS\u2010LSH, 15 Parvis Ren\u00e9 Descartes 69366 Lyon cedex 07 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Paul","family":"Oudinet","sequence":"additional","affiliation":[{"name":"Centre de Biog\u00e9ographie\u2010Ecologie FRE 2545 CNRS\/ENS\u2010LSH, 15 Parvis Ren\u00e9 Descartes 69366 Lyon cedex 07 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"125","volume-title":"Applied geomorphology","author":"Ang\u00e9liaume A.","year":"2002"},{"key":"e_1_2_7_3_1","first-page":"107","article-title":"Ruissellement, \u00e9rosion et qualit\u00e9 des eaux en terre de grande culture. 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