{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:06:36Z","timestamp":1791306396397,"version":"4.3.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2003\u201035102\u201013519"],"award-info":[{"award-number":["2003\u201035102\u201013519"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Env Quality"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There is growing concern that antibiotic use in livestock production is contributing to contamination of soil and aquatic environments. Monensin, a polyether ionophore antibiotic, accounts for approximately 13% of total subtherapeutic livestock antibiotic usage in the USA and has been widely detected in aquatic environments. Due to insufficient ultraviolet absorbance, liquid chromatography analysis of monensin in environmental samples is limited to equipment with mass spectrometry (e.g., liquid chomatography\u2013mass spectrometry [LC\u2010MS]). However, LC\u2010MS equipment is costly, and extensive sample preparation and clean\u2010up is often required. Rapid, low\u2010cost analytical techniques are needed to monitor for monensin residues in the environment. In this study, a commercially available enzyme\u2010linked immunosorbent assay (ELISA) for detecting monensin in animal feed extracts was evaluated for determination of monensin in water, soil, and manure. The monensin ELISA was highly sensitive, with limits of detection and quantification at 1.5 and 3.0 \u03bcg L<jats:sup>\u22121<\/jats:sup>, respectively. Recovery of monensin in spiked water samples was approximately 100%. Cross\u2010reactivity was not observed with similar polyether ionophores, tetracyclines, macrolides, or sulfonamides. Concentrations of monensin using ELISA were greater than concentrations measured with LC\u2010MS. This is attributed to cross\u2010reactivity of the monensin ELISA toward structurally similar monensin compounds, such as factors (slight naturally produced structural variants) and metabolites. Results from this study showed that ELISA can be a reliable, rapid, and low\u2010cost alternative to LC\u2010MS analysis of environmental samples.<\/jats:p>","DOI":"10.2134\/jeq2007.0394","type":"journal-article","created":{"date-parts":[[2008,5,3]],"date-time":"2008-05-03T22:03:16Z","timestamp":1209852196000},"page":"1220-1226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Application of Enzyme\u2010Linked Immunosorbent Assay Analysis for Determination of Monensin in Environmental Samples"],"prefix":"10.1002","volume":"37","author":[{"given":"Holly","family":"Dolliver","sequence":"first","affiliation":[{"name":"Dep. of Plant and Earth Science Univ. of Wisconsin\u2010River Falls 410 S. 3rd St. River Falls WI 54022"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuldip","family":"Kumar","sequence":"additional","affiliation":[{"name":"Res. and Dev. Metropolitan Water Reclamation District of Greater Chicago 6001 West Pershing Rd. Cicero IL 60804"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish","family":"Gupta","sequence":"additional","affiliation":[{"name":"Dep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle St. Paul MN 55108"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Singh","sequence":"additional","affiliation":[{"name":"Veterinary Population Medicine Univ. of Minnesota 1333 Gortner Ave. St. Paul MN 55108"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/b301630g"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050415"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1897\/02-576"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1897\/04-657R.1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.12.091"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/a606833b"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/a805159c"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2005.0348"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1984.5861528x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf60219a005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00044-8"},{"key":"e_1_2_8_13_1","volume-title":"Technical Report","author":"Elanco Products Company","year":"1989"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/a708698i"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-0199-y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19980731)12:14<903::AID-RCM253>3.0.CO;2-V"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2006.04.036"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X2001000400022"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2004.2500"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2113(05)87001-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2005.11.015"},{"key":"e_1_2_8_22_1","volume-title":"Hogging it: Estimates of antimicrobial abuse in livestock","author":"Mellon M.","year":"2001"},{"key":"e_1_2_8_23_1","volume-title":"Feed additive compendium","author":"Miller Publishing Company","year":"2006"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1093\/jaoac\/77.4.885","article-title":"Determination of monensin in chicken tissues by liquid chromatography with postcolumn derivatization","volume":"77","author":"Moran J.W.","year":"1994","journal-title":"J. AOAC Int."},{"key":"e_1_2_8_25_1","first-page":"201","article-title":"Production of antibiodies and development of enzyme immunoassay for determination of monensin in biological samples","volume":"70","author":"Mount M.E.","year":"1987","journal-title":"J. Assoc. Off. Anal. Chem."},{"key":"e_1_2_8_26_1","first-page":"169","article-title":"Monensin concentrations measured in feeder cattle using enzyme immunoassay","volume":"38","author":"Mount M.E.","year":"1996","journal-title":"Vet. Hum. Toxicol."},{"key":"e_1_2_8_27_1","volume-title":"Aquatic toxicity of veterinary pharmaceuticals","author":"Ramsdell H.","year":"2003"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00019-6"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2003.1591"}],"container-title":["Journal of Environmental Quality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2134%2Fjeq2007.0394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.2134\/jeq2007.0394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.2134\/jeq2007.0394","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.2134\/jeq2007.0394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:46:11Z","timestamp":1760625971000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/10.2134\/jeq2007.0394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["10.2134\/jeq2007.0394"],"URL":"https:\/\/doi.org\/10.2134\/jeq2007.0394","archive":["Portico"],"relation":{},"ISSN":["0047-2425","1537-2537"],"issn-type":[{"value":"0047-2425","type":"print"},{"value":"1537-2537","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]},"assertion":[{"value":"2007-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}