{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:55:37Z","timestamp":1762458937375,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Env Quality"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>Wood chip bioreactors are receiving increasing attention as a means of reducing nitrate in subsurface tile drainage systems. Agrochemicals in tile drainage water entering wood chip bioreactors can be retained or degraded and may affect denitrification. The degradation of 5 mg L<jats:sup>\u22121<\/jats:sup> atrazine, enrofloxacin, and sulfamethazine under denitrifying conditions in wood chips from an in situ reactor was determined. The impact of these chemicals on denitrifying microorganisms was assessed using the denitrification potential assay, most probable number (MPN), and quantitative polymerase chain reaction targeting the <jats:italic>nosZ<\/jats:italic> gene of the denitrifiers. Initial half\u2010lives for these chemicals in the aqueous phase were 0.98 d for atrazine, 0.17 d for enrofloxacin, and 6.2 d for sulfamethazine. Similar rates of disappearance in autoclaved and nonautoclaved wood chip solutions during the first 48 h suggested sorption was the dominant mechanism. The presence of atrazine did not impair denitrification potential, the MPN, or the <jats:italic>nosZ<\/jats:italic> copy number. The denitrifier MPN and <jats:italic>nosZ<\/jats:italic> copy number in sulfamethazine\u2010 and enrofloxacin\u2010treated microcosms were less than the control within the first 5 d after chemical addition, whereas the denitrification potentials were not affected. However, after 45 d the denitrification rate, MPN and <jats:italic>nosZ<\/jats:italic> gene copy numbers for sulfamethazine and enrofloxacin were similar to that of the no\u2010chemical control, indicating that acclimation of the denitrifier population to the antibiotic or reduced bioavailability over time allowed recovery of the denitrifier population.<\/jats:p>","DOI":"10.2134\/jeq2011.0082","type":"journal-article","created":{"date-parts":[[2011,12,5]],"date-time":"2011-12-05T14:43:34Z","timestamp":1323096214000},"page":"1816-1823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Dissipation of Atrazine, Enrofloxacin, and Sulfamethazine in Wood Chip Bioreactors and Impact on Denitrification"],"prefix":"10.1002","volume":"40","author":[{"given":"Z. E.","family":"Ilhan","sequence":"first","affiliation":[{"name":"Environmental Science Program Iowa State Univ. Ames IA 50011"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. K.","family":"Ong","sequence":"additional","affiliation":[{"name":"Environmental Science Program Iowa State Univ. Ames IA 50011"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. B.","family":"Moorman","sequence":"additional","affiliation":[{"name":"USDA\u2013ARS, National Lab. for Agriculture and Environment 2110 University Blvd. Ames IA 50011\u20103120"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf063709j"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2009.03.032"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2006.01.029"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1993.00472425002200030024x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1987.tb02454.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2004.10.038"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051223"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2008.10.001"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1995.00472425002400020006x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2008.02.012"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0015265"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac025762m"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2005.0247"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2008.0413"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1897\/04-182R.1"},{"key":"e_1_2_7_17_1","first-page":"121","volume-title":"Veterinary pharmaceuticals in the environment. ACS Symp","author":"Henderson K.L.D.","year":"2009"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00231-06"},{"key":"e_1_2_7_19_1","unstructured":"Ilhan Z.E..2010.Fate of agrochemicals in wood chip denitrifying reactors and their impacts on wood chip microbial ecology. Masters thesis Iowa State Univ. Ames."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1994.00472425002300020014x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2001.3041305x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2007.0279"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2006.01.006"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1093-0191(00)00022-8"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(01)00009-4"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1897\/03-374"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1999.00472425002800030033x"},{"key":"e_1_2_7_28_1","first-page":"991","article-title":"Denitrification within the genus Azospirillum and other associative bacteria","volume":"28","author":"Kloos K.A.","year":"2001","journal-title":"Aust. J. Plant Physiol."},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00374-009-0434-3"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2007.08.020"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1897\/03-582R.1"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1909"},{"key":"e_1_2_7_33_1","first-page":"99","article-title":"Hydrolysis kinetics of atrazine and influence factors","volume":"13","author":"Lei Z.F.","year":"2001","journal-title":"J. Environ. Sci. (China)"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1897\/04-093R.1"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.11.4206-4209.1996"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoleng.2010.03.012"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2664-2667.2000"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1897\/02-351"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0182-8"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2009.02.009"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoleng.2010.04.008"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2005.01.023"},{"key":"e_1_2_7_43_1","first-page":"245","volume-title":"Methods of soil analysis. Part 2. Microbiological and biochemical properties","author":"Tiedje J.M.","year":"1994"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/es103605e"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(95)00242-1"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf052216w"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2008.04.053"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.11.4272-4281.1997"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1989.00472425001800010007x"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/03601230902728245"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2008.04.004"}],"container-title":["Journal of Environmental Quality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2134%2Fjeq2011.0082","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.2134\/jeq2011.0082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:03:22Z","timestamp":1760641402000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/10.2134\/jeq2011.0082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.2134\/jeq2011.0082"],"URL":"https:\/\/doi.org\/10.2134\/jeq2011.0082","archive":["Portico"],"relation":{},"ISSN":["0047-2425","1537-2537"],"issn-type":[{"type":"print","value":"0047-2425"},{"type":"electronic","value":"1537-2537"}],"subject":[],"published":{"date-parts":[[2011,11]]},"assertion":[{"value":"2011-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}