{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T10:43:14Z","timestamp":1788000194317,"version":"build-2784847793"},"reference-count":41,"publisher":"Informa UK Limited","issue":"6","license":[{"start":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T00:00:00Z","timestamp":1586822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"INBRE"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":["BioTechniques"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.2144\/btn-2019-0124","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T04:55:45Z","timestamp":1586840145000},"page":"318-324","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":15,"title":["Optimization of Western Blotting for the Detection of Proteins of Different Molecular Weight"],"prefix":"10.1080","volume":"68","author":[{"given":"Ghanshyam D","family":"Heda","sequence":"first","affiliation":[{"name":"Department of Sciences & Mathematics,Mississippi University for Women,\rColumbus,\rMS 39701,\rUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Shrestha","sequence":"additional","affiliation":[{"name":"Department of Sciences & Mathematics,Mississippi University for Women,\rColumbus,\rMS 39701,\rUSA"},{"name":"Department of Genetics,The University of Alabama at Birmingham,\rBirmingham,\rAL 35294,\rUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sagarina","family":"Thapa","sequence":"additional","affiliation":[{"name":"Department of Sciences & Mathematics,Mississippi University for Women,\rColumbus,\rMS 39701,\rUSA"},{"name":"Molecular Medicine,University of Maryland School of Medicine,\rBaltimore,\rMD 21201,\rUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shreya","family":"Ghimire","sequence":"additional","affiliation":[{"name":"Department of Sciences & Mathematics,Mississippi University for Women,\rColumbus,\rMS 39701,\rUSA"},{"name":"Department of Internal Medicine,The University of Iowa,\rIowa City,\rIA 52240,\rUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diptika","family":"Raut","sequence":"additional","affiliation":[{"name":"Department of Sciences & Mathematics,Mississippi University for Women,\rColumbus,\rMS 39701,\rUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2020,4,14]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90281-5"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5350"},{"key":"e_1_3_3_6_1","first-page":"25","article-title":"Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin","volume":"418","author":"Zwart SR","year":"2008","unstructured":"Zwart SR , LewisBJ. Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin. Methods Mol. Biol.418, 25\u201334 (2008).","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2011.06.001"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2694-7_26"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201900059"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471143030.cb0602s52"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"issue":"2","key":"e_1_3_3_12_1","first-page":"85","article-title":"Western blotting of transforming growth factor beta 2. Optimization of the electrophoretic transfer","volume":"3","author":"Jin Y","year":"1992","unstructured":"Jin Y , CerlettiN. Western blotting of transforming growth factor beta 2. Optimization of the electrophoretic transfer. Appl. Theor. Electrophor.3(2), 85\u201390 (1992).","journal-title":"Appl. Theor. Electrophor."},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2000)086[0637:DOHSPF]2.0.CO;2"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.34"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2017_2299"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2019.01.018"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2694-7_5"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02789065"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-0651(84)90049-9"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2012.03.028"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90245-2"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90379-2"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90056-2"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1068"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90089-8"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-002-1_6"},{"issue":"4","key":"e_1_3_3_27_1","first-page":"397","article-title":"Influence of SDS and methanol on protein electrotransfer to Immobilon P membranes in semidry blot systems","volume":"9","author":"Lissilour S","year":"1990","unstructured":"Lissilour S , GodinotC. Influence of SDS and methanol on protein electrotransfer to Immobilon P membranes in semidry blot systems. BioTechniques9(4), 397\u2013398400\u2013401 (1990).","journal-title":"BioTechniques"},{"issue":"9","key":"e_1_3_3_28_1","first-page":"S1","article-title":"Toxic alcohol ingestion: prompt recognition and management in the emergency department [digest]","volume":"18","author":"Beauchamp GA","year":"2016","unstructured":"Beauchamp GA , ValentoM, KimJ. Toxic alcohol ingestion: prompt recognition and management in the emergency department [digest]. Emerg. Med. Pract.18(Suppl. 9 Point & Pearls), S1\u2013S2 (2016).","journal-title":"Emerg. Med. Pract."},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpmed.2007.09.014"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2012.07.002"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.096669"},{"key":"e_1_3_3_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2684"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2013.10.011"},{"key":"e_1_3_3_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2004.05.017"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.7171\/jbt.16-2703-004"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2475911"},{"key":"e_1_3_3_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54636-6"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90478-9"},{"issue":"1","key":"e_1_3_3_39_1","first-page":"45","article-title":"Stability of CFTR on plasma membrane of a lung epithelial cell line","volume":"64","author":"Thapa S","year":"2019","unstructured":"Thapa S , RautD, ShresthaL, GhimireS, HedaGD. Stability of CFTR on plasma membrane of a lung epithelial cell line. J. Miss. Acad. Sci.64(1), 45\u201346 (2019).","journal-title":"J. Miss. Acad. Sci."},{"key":"e_1_3_3_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-016-1155-9"},{"key":"e_1_3_3_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)33716-5"},{"key":"e_1_3_3_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90128-8"}],"container-title":["BioTechniques"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.tandfonline.com\/doi\/pdf\/10.2144\/btn-2019-0124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T15:12:20Z","timestamp":1716304340000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.tandfonline.com\/doi\/full\/10.2144\/btn-2019-0124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,14]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.2144\/btn-2019-0124"],"URL":"https:\/\/doi.org\/10.2144\/btn-2019-0124","relation":{},"ISSN":["0736-6205","1940-9818"],"issn-type":[{"value":"0736-6205","type":"print"},{"value":"1940-9818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,14]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=ibtn20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=ibtn20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"},{"value":"2019-09-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}