{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T11:06:37Z","timestamp":1759057597580,"version":"3.40.5"},"reference-count":35,"publisher":"Informa UK Limited","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IMCRJ"],"DOI":"10.2147\/imcrj.s291166","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T23:41:48Z","timestamp":1610322108000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Partial N Gene Sequencing for SARS-CoV-2 Verification and Pathway Tracing"],"prefix":"10.2147","volume":"Volume 14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1762-1060","authenticated-orcid":true,"given":"Sin Hang","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8227-0976","authenticated-orcid":true,"given":"Jonathan","family":"McGrath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P","family":"Connolly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2021,1]]},"reference":[{"key":"ref1","unstructured":"WHO Coronavirus disease (COVID-19) weekly epidemiological update and weekly operational update; December 15, 2020. Available from: https:\/\/www.who.int\/emergencies\/diseases\/novel-coronavirus-2019\/situation-reports. Accessed December 19, 2020."},{"key":"ref2","unstructured":"CDC. 2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel. Instructions for use. Available from: https:\/\/www.fda.gov\/media\/134922\/download. Accessed November 2, 2020."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1016\/S2213-2600(20)30453-7","volume":"8","author":"Surkova","year":"2020","journal-title":"Lancet Respir Med"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"e38","DOI":"10.1056\/NEJMp2015897","volume":"383","author":"Woloshin","year":"2020","journal-title":"N Engl J Med"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"E32","DOI":"10.1148\/radiol.2020200642","volume":"296","author":"Ai","year":"2020","journal-title":"Radiology"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"00324","DOI":"10.1183\/23120541.00324-2020","volume":"6","author":"Di Paolo","year":"2020","journal-title":"ERJ Open Res"},{"key":"ref7","unstructured":"Patra K. Around the NFL- All 77 false-positive COVID-19 tests come back negative upon reruns; August 24, 2020. Available from: https:\/\/www.nfl.com\/news\/all-77-false-positive-covid-19-tests-come-back-negative-upon-reruns. Accessed November 2, 2020."},{"key":"ref8","unstructured":"FDA. False positive results with BD SARS-CoV-2 reagents for the BD max system - letter to clinical laboratory staff and health care providers. Available from: https:\/\/www.fda.gov\/medical-devices\/letters-health-care-providers\/false-positive-results-bd-sars-cov-2-reagents-bd-max-system-letter-clinical-laboratory-staff-and. Accessed November 2, 2020."},{"key":"ref9","unstructured":"FDA. Risk of inaccurate results with thermo fisher scientific TaqPath COVID-19 combo kit - letter to clinical laboratory staff and health care providers. Available from: https:\/\/www.fda.gov\/medical-devices\/letters-health-care-providers\/risk-inaccurate-results-thermo-fisher-scientific-taqpath-covid-19-combo-kit-letter-clinical?utm_campaign=2020-08-17%20Risk%20of%20Inaccurate%20Results%20with%20Thermo%20Fisher%20Scientific%20TaqPath&utm_medium=email&utm_source=Eloqua. Accessed November 2, 2020."},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.10.003","author":"Skittrall","year":"2020","journal-title":"Clin Microbiol Infect"},{"key":"ref11","unstructured":"WHO. Laboratory testing for coronavirus disease (COVID-19) in suspected human cases-Interim guidance 19 March 2020. Available from: https:\/\/www.who.int\/publications\/i\/item\/10665-331501. Accessed November 2, 2020."},{"key":"ref12","unstructured":"Open letter from FDA to Robert R. Redfield, MD, Director, Centers for Disease Control and Prevention; March 15, 2020. Available from: https:\/\/www.fda.gov\/media\/134919\/download. Accessed November 2, 2020."},{"key":"ref13","unstructured":"FDA. Molecular diagnostic template for laboratories. Policy for coronavirus disease-2019 tests during the public health emergency (Revised) Available from: https:\/\/www.fda.gov\/media\/135659\/download. Accessed November 2, 2020."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"2401","DOI":"10.3201\/eid2610.201800","volume":"26","author":"Paden","year":"2020","journal-title":"Emerg Infect Dis"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"ciaa1275","DOI":"10.1093\/cid\/ciaa1275","author":"To","year":"2020","journal-title":"Clin Infect Dis"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"ciaa1330","DOI":"10.1093\/cid\/ciaa1330","author":"Van Elslande","year":"2020","journal-title":"Clin Infect Dis"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"9241","DOI":"10.1073\/pnas.2004999117","volume":"117","author":"Forster","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","unstructured":"FDA. Establishing the performance characteristics of in vitro diagnostic devices for the detection or detection and differentiation of human papillomaviruses. Available from: https:\/\/www.fda.gov\/media\/92930\/download. Accessed November 2, 2020."},{"key":"ref19","first-page":"69","volume":"2","author":"Lee","year":"2020","journal-title":"Int J Geriatr Rehabil"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"495","DOI":"10.2471\/BLT.20.253591","volume":"98","author":"Koyama","year":"2020","journal-title":"Bull World Health Organ"},{"key":"ref21","unstructured":"China National Center for Bioinformation 2019 Novel Coronavirus Resource (2019nCoVR). Available from: https:\/\/bigd.big.ac.cn\/ncov\/variation\/annotation. Accessed November 4, 2020."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"3541361","DOI":"10.2139\/ssrn.3541361","author":"Grifoni","year":"2020","journal-title":"SSRN"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1001\/jama.2020.2648","volume":"323","author":"Wu","year":"2020","journal-title":"JAMA"},{"key":"ref24","unstructured":"Becton, Dickinson and Company. CerTest Biotec and BD announce COVID-19 diagnostic test. Available from: https:\/\/news.bd.com\/2020-03-10-CerTest-Biotec-and-BD-Announce-COVID-19-Diagnostic-Test. Accessed November 2, 2020."},{"key":"ref25","unstructured":"BBC News. Coronavirus: first case confirmed in Republic of Ireland; February 29, 2020. Available from: https:\/\/www.bbc.com\/news\/world-europe-51693259. Accessed November 2, 2020."},{"key":"ref26","unstructured":"CDC. Coronavirus disease 2019 (COVID-19). Available from: https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/symptoms-testing\/symptoms.html?CDC_AA_refVal=https%3A%2F%2Fwww.cdc.gov%2Fcoronavirus%2F2019-ncov%2Fabout%2Fsymptoms.html. Accessed November 2, 2020."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"4433","DOI":"10.1007\/s00018-016-2299-6","volume":"73","author":"Sanju\u00e1n","year":"2016","journal-title":"Cell Mol Life Sci"},{"key":"ref28","unstructured":"Kelly M, Cahlan S, Samuels E. What went wrong with coronavirus testing in the US. Washington Post; March 30, 2020. Available from: https:\/\/www.washingtonpost.com\/politics\/2020\/03\/30\/11-100000-what-went-wrong-withcoronavirus-testing-us\/. Accessed November 3, 2020."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1026","DOI":"10.1038\/nbt0993-1026","volume":"11","author":"Higuchi","year":"1993","journal-title":"Biotechnology"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1385\/MB:14:1:01","volume":"14","author":"Ryu","year":"2000","journal-title":"Mol Biotechnol"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1186\/1756-0500-3-273","volume":"3","author":"Lee","year":"2010","journal-title":"BMC Res Notes"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"1214","DOI":"10.4236\/abb.2012.38148","volume":"3","author":"Lee","year":"2012","journal-title":"Adv Biosci Biotechnol"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1111\/j.1600-0463.2008.00010.x","volume":"117","author":"Nowrouzian","year":"2009","journal-title":"APMIS"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.bdq.2017.10.001","volume":"14","author":"Ruiz-Villalba","year":"2017","journal-title":"Biomol Detect Quantif"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1086\/324490","volume":"33","author":"Nowakowski","year":"2001","journal-title":"Clin Infect Dis"}],"container-title":["International Medical Case Reports Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.dovepress.com\/getfile.php?fileID=65562","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.dovepress.com\/getfile.php?fileID=65562","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T23:41:57Z","timestamp":1610322117000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.dovepress.com\/partial-n-gene-sequencing-for-sars-cov-2-verification-and-pathway-trac-peer-reviewed-article-IMCRJ"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":35,"URL":"https:\/\/doi.org\/10.2147\/imcrj.s291166","relation":{},"ISSN":["1179-142X"],"issn-type":[{"type":"electronic","value":"1179-142X"}],"subject":[],"published":{"date-parts":[[2021,1]]}}}