{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:23:54Z","timestamp":1767842634288,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":2798,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2005,5,6]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> Mammalian members of the cysteine\u2010rich secretory protein (CRISP) family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation, and capacitation to oocyte\u2010sperm fusion, and possibly also penetration of the zona pellucida. Rodents express only 2 CRISPs (CRISP\u20101 and CRISP\u20102) in their male reproductive system, whereas humans and horses express an additional third member named CRISP\u20103. We have previously demonstrated that this protein is present in human seminal plasma as well as in other exocrine secretions, in blood plasma, and in neutrophilic granulocytes. To characterize the protein in seminal plasma and localize the production of CRISP\u20103 in the human male reproductive tract, we performed immunoblotting and enzyme\u2010linked immunosorbent assay measurements of seminal plasma and immunohistochemistry and in situ hybridization of tissue specimens. We were able to show that human CRISP\u20103 is a quantitatively minor seminal plasma protein not associated with prostasomes. Furthermore, CRISP\u20103 expression was found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. Examination of seminal plasma from vasectomized males indicates that organs downstream of the epididymis are probably the major sources of seminal plasma CRISP\u20103.<\/jats:p>","DOI":"10.2164\/jandrol.04132","type":"journal-article","created":{"date-parts":[[2005,5,2]],"date-time":"2005-05-02T19:28:15Z","timestamp":1115062095000},"page":"333-342","source":"Crossref","is-referenced-by-count":71,"title":["Characterization and Localization of Cysteine\u2010Rich Secretory Protein 3 (CRISP\u20103) in the Human Male Reproductive 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