{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:11:17Z","timestamp":1767849077094,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":2608,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2005,11,12]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> Vasectomy results in the occlusion of testicular outflow, leading to autoimmunity characterized by the production of antisperm antibodies (ASA). Reports on the rise in ASA following vasectomy in several species are available; however, not much is known about the specific sperm autoantigens to which postvasectomy antibodies are directed. In the present study, monoclonal antibodies were generated using a vasectomized mouse. One of the monoclonal antibodies, D5E5, identified an approximately 70\u2010kd antigen localized on the principal piece of the tail and also on the tip of the acrosome of mouse sperm. The cognate antigen was expressed postmeiotically in a stage\u2010specific manner during spermiogenesis, starting from step 8 of elongating spermatids during spermiogenesis up to mature spermatozoa. The protein was conserved across the species, as observed by its presence in rat, bull, marmoset, and human sperm. Following capacitation, the antigen on the head was seen to shift to the acrosomal region and was lost after the acrosome reaction. However, the localization on tip of the acrosome still persisted, which indicates that the antigen may play a role post\u2010acrosome reaction in sperm egg interaction. Resistance to Triton X\u2010100 solubilization indicates that TSA70 could be an acrosomal matrix protein. In addition, we observed a significant reduction in forward progressive motility of mouse sperm treated in vitro with D5E5. In view of its testis specificity, acrosome and tail localization, and conserved nature, TSA70 is likely to play an important role in sperm function.<\/jats:p>","DOI":"10.2164\/jandrol.05045","type":"journal-article","created":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T21:57:46Z","timestamp":1132178266000},"page":"761-771","source":"Crossref","is-referenced-by-count":5,"title":["Monoclonal Antibody From Vasectomized Mouse Identifies a Conserved Testis\u2010Specific Antigen TSA70"],"prefix":"10.1002","volume":"26","author":[{"given":"Monali S.","family":"Wakle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saurabh A.","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vrinda V.","family":"Khole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1988.tb04367.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)47724-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-7824(91)90158-C"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01303.x"},{"key":"e_1_2_6_6_1","first-page":"86","volume-title":"Methods in Mammalian Embryology","author":"Biggers JS","year":"1971"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)46701-4"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0015-0282(16)48009-X","article-title":"Sperm antibodies: their role in infertility","volume":"42","author":"Bronson RA","year":"1984","journal-title":"Fertil Steril"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10092"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1252"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.4.984"},{"key":"e_1_2_6_12_1","first-page":"569","article-title":"Association of egg zona pellucida glycoprotein mZP3 with sperm protein sp56 during fertilization in mice","volume":"45","author":"Cohen N","year":"2001","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a138102"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1991.tb01606.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(94)90029-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod64.5.1451"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1986.tb00932.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.1.41"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1998.tb00348.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod39.5.1239"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(90)90039-9"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod37.5.1297"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod32.2.451"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01335-8"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(90)90304-2"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.8755-8920.2000.440606.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod64.1.36"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1990.tb00194.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1983.tb00243.x"},{"key":"e_1_2_6_32_1","first-page":"413","article-title":"Sperm agglutinins in seminal plasma and serum after vasectomy: correlation between immunological and clinical findings","volume":"30","author":"Linnet L","year":"1977","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(95)00927-D"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.5.1634"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2837"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod41.1.163"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2795(200101)58:1<116::AID-MRD14>3.0.CO;2-8"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1998.tb01161.x"},{"key":"e_1_2_6_40_1","first-page":"252","article-title":"Studies on the immunogenicity of protamines in humans and experimental animals by means of a micro complement fixation test","volume":"33","author":"Samuel T","year":"1978","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_41_1","first-page":"261","article-title":"Post vasectomy autoimmunity to protamine in relation to the formation of granulomas and sperm agglutinating antibodies","volume":"33","author":"Samuel T","year":"1978","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.021667"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.032623"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(89)90034-X"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1989.tb00648.x"}],"container-title":["Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2164%2Fjandrol.05045","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.2164\/jandrol.05045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T23:49:02Z","timestamp":1697154542000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.2164\/jandrol.05045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,12]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,11,12]]}},"alternative-id":["10.2164\/jandrol.05045"],"URL":"https:\/\/doi.org\/10.2164\/jandrol.05045","archive":["Portico"],"relation":{},"ISSN":["0196-3635","1939-4640"],"issn-type":[{"value":"0196-3635","type":"print"},{"value":"1939-4640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11,12]]}}}