{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:00:35Z","timestamp":1791352835586,"version":"4.3.1"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":2306,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2006,9,10]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> The receptor mechanism of testosterone\u2010induced nongenomic Ca<jats:sup>2+<\/jats:sup> signaling in prostate cancer cells is poorly understood. In this study we investigated androgen\u2010induced intracellular Ca<jats:sup>2+<\/jats:sup> increases in LNCaP human prostate cancer cells with Fura\u20102 as a Ca<jats:sup>2+<\/jats:sup> probe. 5\u03b1\u2010dihydrotestosterone (DHT) produced fast and transient increases in intracellular Ca<jats:sup>2+<\/jats:sup> in LNCaP cells in a concentration\u2010dependent manner. These effects were abolished by extracellular Ca<jats:sup>2+<\/jats:sup> removal or pretreatment with L\u2010type Ca<jats:sup>2+<\/jats:sup> channel inhibitors (nifedipine, verapamil, and diltiazem). Pretreatment with endoplasmic reticulum ryanodine receptor blocker (procaine) or phospholipase C inhibitor (neomycin sulfate) did not alter DHT\u2010induced Ca<jats:sup>2+<\/jats:sup> influx. The concentration of Ca<jats:sup>2+<\/jats:sup> was also increased by impermeable testosterone conjugated to bovine serum albumin. Neither an antagonist of intracellular androgen receptors (cyproterone acetate) nor a protein synthesis inhibitor (cycloheximide) affected this fast Ca<jats:sup>2+<\/jats:sup> influx. Furthermore, the effect of DHT was abolished in cells incubated with a G protein inhibitor (pertussis toxin) and a nonhydrolyzable analog of guanosine triphosphate (guanosine 5\u2010[b\u2010thio]disphosphate) but not in cells incubated with the tyrosine kinase inhibitor genistein. These results indicate that androgens induced an L\u2010type calcium channel\u2014dependent intracellular Ca<jats:sup>2+<\/jats:sup> increase in LNCaP prostate cancer cells. The rapid responses triggered by DHT did not appear to be mediated through classic intracellular androgen receptors, c\u2010Src kinase\u2010androgen receptor complex, or sex hormone\u2014binding globulin but through a G protein\u2014coupled receptor in LNCaP prostate cancer cells. These results may provide a new explanation for progression of prostate cancer.<\/jats:p>","DOI":"10.2164\/jandrol.106.000554","type":"journal-article","created":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T15:54:11Z","timestamp":1157126051000},"page":"671-678","source":"Crossref","is-referenced-by-count":67,"title":["Androgens Induce Increases in Intracellular Calcium Via a G Protein\u2014Coupled Receptor in LNCaP Prostate Cancer Cells"],"prefix":"10.1002","volume":"27","author":[{"given":"Ying\u2010Hao","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Jie","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan\u2010Liang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin\u2010Hui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.1.123"},{"key":"e_1_2_5_3_1","first-page":"907","article-title":"Cloning and tissue expression of two putative steroid membrane receptors","volume":"379","author":"Gerdes D.","year":"1998","journal-title":"Biol Chem"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.136.5.2172"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.136.5.2052"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.20.1.399"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0070"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.1057"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0131fje"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.64.3.415"},{"key":"e_1_2_5_12_1","first-page":"6039","article-title":"Activation of mitogen\u2010activated protein kinase pathway by the antiandrogen hydroxyflutamide in androgen receptor\u2010negative prostate cancer cells","volume":"62","author":"Lee YF","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"7219","DOI":"10.1016\/S0021-9258(17)37270-8","article-title":"Androgens increase intracellular calcium concentrations and inositol 1,4,5\u2010trisphosphate and diacylglycerol formation via a pertussis toxin sensitive G protein","volume":"269","author":"Lieberherr M.","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1210\/jcem.83.1.4523","article-title":"Nongenomic effects of androstenedione on human granulose luteinizing cells","volume":"83","author":"Machelon V.","year":"1998","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(98)00108-1"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.0.0100209"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91338-D"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0045(20000601)43:4<303::AID-PROS10>3.0.CO;2-V"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.59.1.365"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(01)00175-1"}],"container-title":["Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2164%2Fjandrol.106.000554","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.2164\/jandrol.106.000554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:39:49Z","timestamp":1697096389000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.2164\/jandrol.106.000554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,10]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,9,10]]}},"alternative-id":["10.2164\/jandrol.106.000554"],"URL":"https:\/\/doi.org\/10.2164\/jandrol.106.000554","archive":["Portico"],"relation":{},"ISSN":["0196-3635","1939-4640"],"issn-type":[{"value":"0196-3635","type":"print"},{"value":"1939-4640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9,10]]}}}