{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T21:11:24Z","timestamp":1774559484646,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":2068,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2007,5,6]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> To explore the biological characteristics of the recombinant zona pellucida 3 (ZP3) peptides of rhuZP3a<jats:sup>22\u223c176<\/jats:sup> and rhuZP3b<jats:sup>177\u223c348<\/jats:sup>, we examined whether rhuZP3a<jats:sup>22\u223c176<\/jats:sup> or rhuZP3b<jats:sup>177\u223c348<\/jats:sup> trigger the acrosome reaction (AR) of human spermatozoa and we investigated the underlying mechanism. The assessment of AR was performed using chlortetracycline staining. The intracellular free calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]i) in Fura\u20102\/AM\u2010loaded human sperm was monitored with a spectrofluorophotometer. We found that rhuZP3a<jats:sup>22\u223c176<\/jats:sup> and rhuZP3b<jats:sup>177\u223c348<\/jats:sup> were capable of eliciting AR at different concentrations. With the addition of either peptide, the [Ca<jats:sup>2+<\/jats:sup>]i level was raised to a peak with or without a plateau. The AR could be inhibited by pertussis toxin (PTX), EGTA, and pimozide (a T\u2010type calcium channel blocker), whereas verapamil was less effective in this regard. The results of the present study suggest that peptides rhuZP3a<jats:sup>22\u223c176<\/jats:sup> and rhuZP3b<jats:sup>177\u223c348<\/jats:sup> have a role similar to human ZP3, and that the mechanism of the response to the peptides involves influx of calcium, the G protein pathway, and a T\u2010type calcium channel.<\/jats:p>","DOI":"10.2164\/jandrol.106.001289","type":"journal-article","created":{"date-parts":[[2007,4,27]],"date-time":"2007-04-27T19:23:53Z","timestamp":1177701833000},"page":"381-388","source":"Crossref","is-referenced-by-count":13,"title":["Acrosome Reaction Induced by Recombinant Human Zona Pellucida 3 Peptides rhuZP3a<sup>22\u223c176<\/sup> and rhuZP3b<sup>177\u223c348<\/sup> and Their Mechanism"],"prefix":"10.1002","volume":"28","author":[{"given":"Ya","family":"Ni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanxiang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwen","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kangshou","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hefeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Xian","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.13004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.12.6757"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.3.637"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"15041","DOI":"10.1016\/S0021-9258(18)48135-5","article-title":"Independent 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