{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T12:52:04Z","timestamp":1773060724675,"version":"3.50.1"},"reference-count":70,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1337,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2009,5,6]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> Cryptorchidism and scrotal heating result in abnormal spermatogenesis, but the mechanism(s) prescribing this temperature sensitivity are unknown. It was previously reported that the AKR\/N or MRL\/MpJ\u2010+\/+ mouse testis is more heat\u2010resistant than the testis from the C57BL\/6 strain. We have attempted to probe into the mechanism(s) involved in heat sensitivity by examining global gene expression profiles of normal and heat\u2010treated testes from C57BL\/6, AKR\/N, and MRL\/MpJ\u2010+\/+ mice by microarray analysis. In the normal C57BL\/6 testis, 415 and 416 transcripts were differentially expressed (at least 2\u2010fold higher or lower) when compared with the normal AKR\/N and MRL\/MpJ\u2010+\/+ testis, respectively. The AKR\/N and MRL\/MpJ\u2010+\/+ strains revealed 268 differentially expressed transcripts between them. There were 231 transcripts differentially expressed between C57BL\/6 and 2 purported heat\u2010resistant strains, AKR\/N and MRL\/MpJ\u2010+\/+. Next, the testes of C57BL\/6 and AKR\/N mice were exposed to 43\u00b0C for 15 minutes and harvested at different time points for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) studies and microarrays. An increase of TUNEL\u2010positive germ cell numbers was significant 8 hours after heat exposure in the C57BL\/6 mouse. However, this increase was not observed in the AKR\/N mouse until 10 hours after heat exposure. All tubules showed germ cell loss and disruption in C57BL\/6 testis 24 hours after heat shock. In contrast, although a number of seminiferous tubules showed an abnormal morphology 24 hours post\u2014heat shock in the AKR\/N mouse, many tubules still retained a normal structure. Numerous transcripts exhibited differential regulation between the 2 strains within 24 hours after heat exposure. The differentially expressed transcripts in the testes 8 hours after heat exposure were targeted to identify the genes involved in the initial response rather than those attributable to germ cell loss. Twenty transcripts were significantly down\u2010regulated and 19 genes were up\u2010regulated by hyperthermia in C57BL\/6 and did not show a parallel change in the AKR\/N testis. Conversely, heat shock resulted in 30 up\u2010regulated transcripts and 31 down\u2010regulated transcripts in AKR\/N that were not similarly regulated in C57BL\/6. A number of genes shared similar differential expression patterns and differential regulation by hyperthermia in both strains of mice. Taken together, the results of the present study indicate that the diverse genetic backgrounds in the 3 strains lead to major differences in normal testis gene expression profiles, whereas the differences in heat shock responses involve a significantly smaller number of genes. The data generated may provide insights regarding gene networks and pathways involved in heat stress and their relationship to spermatogenesis.<\/jats:p>","DOI":"10.2164\/jandrol.108.005934","type":"journal-article","created":{"date-parts":[[2009,5,26]],"date-time":"2009-05-26T17:18:43Z","timestamp":1243358323000},"page":"325-337","source":"Crossref","is-referenced-by-count":29,"title":["Differential Gene Expression in the Testes of Different Murine Strains Under Normal and Hyperthermic 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