{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:04:17Z","timestamp":1783105457831,"version":"3.54.6"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1035,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2010,3,4]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> Swim\u2010up (SU) is a technique that permits the obtaining of motile sperm. Based on the sperm motility of neat ejaculates, we analyzed functional parameters, cytoplasmic esterases, and mitochondrial dehydrogenases of ram sperm using calcein acetomethylester and resazurin, respectively. Active caspase\u20103 and \u22127 and chromatin structure were evaluated in ram sperm before and after the SU process. There were no changes in any of the viability parameters after SU in neat semen samples with less or more than 25% motility. The percentage of active caspase\u20103 and \u22127 decreased after SU (68.8 \u00b1 4.6 vs 54.2 \u00b1 6.0), whereas a small but significant increase of chromatin structural abnormalities was observed (DNA fragmentation index[DFI], 287.3 \u00b1 3.1 vs 297.2 \u00b1 2.4). For the first time, the location of active caspase\u20103 and \u22127 was described for ram sperm. Notably, we found active caspases in the implantation fossa region. The presence of active caspases in neat ejaculates and the diminished presence of active caspases in SU\u2010processed ejaculates suggest a role for caspases in motility and possibly in male fertility. The results of this study indicatethat the evaluation of more than one cell\u2010function marker is necessary to appropriately evaluate sperm quality. Furthermore, in semen samples with low motility, a lower percentage of sperm with active caspases is obtained after SU, although these sperm present increased values of DFI.<\/jats:p>","DOI":"10.2164\/jandrol.108.007021","type":"journal-article","created":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T21:45:27Z","timestamp":1268948727000},"page":"169-176","source":"Crossref","is-referenced-by-count":10,"title":["Viability Tests, Active Caspase\u20103 and \u22127, and Chromatin Structure in Ram Sperm Selected Using the Swim\u2010Up Procedure"],"prefix":"10.1002","volume":"31","author":[{"given":"Ana I.","family":"Pichardo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Arag\u00f3n\u2010Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda 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