{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:15:29Z","timestamp":1792800929407,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1035,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2010,3,4]]},"abstract":"<jats:p><jats:bold>ABSTRACT: <\/jats:bold> This study investigated whether specific proteins from distinct seminal plasma fractions of boars could be related to in vivo fertility. Nine boars with acceptable sperm motility and morphology for use in artificial insemination demonstrated major differences in total number born and pregnancy rate when low sperm doses (1.5 billion sperm) were used to breed a minimum of 50 gilts per boar. The 2 lowest\u2010fertility and 2 highest\u2010fertility boars were chosen for evaluation of specific seminal plasma proteins. On 4 occasions, semen was collected and separated into 3 fractions based on sperm concentration (Sperm\u2010Peak, Sperm\u2010Rich, and Sperm\u2010Free), and the fractions were analyzed for total protein concentration and abundance of major seminal plasma glycoprotein (PSP\u2010I), AWN\u20101, and osteopontin protein using Western blotting techniques. The concentrations of these seminal plasma proteins were lower in the Sperm\u2010Peak fractions compared with the Sperm\u2010Free fractions (<jats:italic>P<\/jats:italic> &lt; .05). Seminal plasma from the pooled Sperm\u2010Rich fraction used for artificial insemination was also subjected to two\u2010dimensional gel electrophoresis to investigate novel protein markers related to in vivo fertility. Total piglets born (<jats:italic>r<\/jats:italic> = \u22120.76, <jats:italic>P<\/jats:italic> = .01) and sperm motility at day 7 (<jats:italic>r<\/jats:italic> = \u22120.74, <jats:italic>P<\/jats:italic> = .037) were again negatively correlated with a 22\u2010kDa protein identified by mass spectrometry as PSP\u2010I. However, fertility index and farrowing rate tended to be positively correlated (<jats:italic>P<\/jats:italic> &lt; .10) with a 25\u2010kDa protein, identified as glutathione peroxidase (GPX5), an antioxidant enzyme that may protect sperm membranes from oxidative damage. These candidate proteins merit further investigation as markers of fertility in boars.<\/jats:p>","DOI":"10.2164\/jandrol.109.007583","type":"journal-article","created":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T21:45:27Z","timestamp":1268948727000},"page":"188-200","source":"Crossref","is-referenced-by-count":79,"title":["Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars"],"prefix":"10.1002","volume":"31","author":[{"given":"Susan","family":"Novak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Ruiz\u2010S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter T.","family":"Dixon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George 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