{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:20:29Z","timestamp":1790349629088,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":178,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2012,7,8]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> We report the first case of a healthy baby born after intrauterine insemination with vitrified swim\u2010up spermatozoa from an oligoasthenozoospermic patient. A 39\u2010year\u2010old patient was subjected to intrauterine insemination with spermatozoa from her 35\u2010year\u2010old husband, diagnosed with oligoasthenozoospermia. The swim\u2010up spermatozoa from 2 ejaculates were suspended in a culture medium supplemented with 1% human serum albumin and 0.25 M sucrose. Three hermetically packaged 100\u2010\u03bcL sperm portions (each containing 1.0 \u00d7 10<jats:sup>6<\/jats:sup> spermatozoa\/mL) were vitrified by direct plunging into liquid nitrogen. The presence of leukocytes in the ejaculates was determined by indirect immunofluorescence assay. Production of reactive oxygen species was measured by chemiluminescent assay. Before intrauterine insemination, all portions were warmed in culture medium at 37\u00b0C, and the spermatozoa were concentrated by centrifugation; they were then resuspended in 500 \u03bcL of culture medium and inseminated. The cell suspension used for insemination displayed 60% progressive motility. Clinical pregnancy was confirmed at 7 weeks of gestation, and a healthy baby was born at term. Our results show that a successful intrauterine insemination can be achieved with aseptic vitrification of spermatozoa.<\/jats:p>","DOI":"10.2164\/jandrol.111.014274","type":"journal-article","created":{"date-parts":[[2012,7,13]],"date-time":"2012-07-13T09:40:55Z","timestamp":1342172455000},"page":"559-562","source":"Crossref","is-referenced-by-count":50,"title":["Live Birth After Intrauterine Insemination With Spermatozoa From an Oligoasthenozoospermic Patient Vitrified Without Permeable Cryoprotectants"],"prefix":"10.1002","volume":"33","author":[{"given":"Raul","family":"Sanchez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Isachenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna M.","family":"Petrunkina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennie","family":"Risopatron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mabel","family":"Schulz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgenia","family":"Isachenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0810459"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)01694-0"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.11003"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012399770-8\/50007-1"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.1.112"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2605.1999.00162.x"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2009.00967.x"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1997.tb00322.x"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.11.022"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-1-108"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61710-5"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh194"},{"key":"e_1_2_4_14_1","first-page":"177","volume-title":"Principles and Practice of Fertility Preservatio","author":"Isachenko E","year":"2011"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0463"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61795-6"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD11061"},{"key":"e_1_2_4_18_1","first-page":"643","article-title":"Cryoprotectant\u2010free vitrification of human spermatozoa in large (to 0.5 mL) volume: a novel technology","volume":"57","author":"Isachenko V","year":"2011","journal-title":"Clin Lab"},{"key":"e_1_2_4_19_1","first-page":"172","article-title":"Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men","volume":"46","author":"Kumar R","year":"2009","journal-title":"Indian J Biochem Biophys"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137176"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.09.035"},{"key":"e_1_2_4_22_1","first-page":"93","article-title":"Vitrification of human spermatozoa without cryoprotectants","volume":"23","author":"Nawroth F","year":"2002","journal-title":"CryoLetters"},{"key":"e_1_2_4_23_1","first-page":"78","article-title":"Fundamental aspects of gamete cryobiology","volume":"4","author":"Petrunkina AM.","year":"2007","journal-title":"J Reproduktionsmed Endokrinol"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1985.tb37520.x"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(99)00255-9"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0303-4569.2001.00476.x"},{"key":"e_1_2_4_27_1","volume-title":"Examination and Processing of Human Seme","author":"World Health Organization (WHO)","year":"2010"},{"key":"e_1_2_4_28_1","first-page":"87","article-title":"Human sperm cryobanking. 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