{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:42:55Z","timestamp":1791114175103,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":178,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Andrology"],"published-print":{"date-parts":[[2012,7,8]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> An isotope dilution mass spectrometry method has been developed for the simultaneous measurement of picolinoyl derivatives of testosterone (T), dihydrotestosterone (DHT), 17\u03b2\u2010estradiol (E<jats:sub>2<\/jats:sub>), and 5\u03b1\u2010androstan\u20103\u03b1,17\u03b2\u2010diol (3\u03b1\u2010diol) in rat intratesticular fluid. The method uses reversed\u2010phase high\u2010performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Following derivatization of 10\u2010\u03bcL samples of testicular fluid with picolinoyl chloride hydrochloride, the samples were purified by solid phase extraction before analysis. The accuracy of the method was satisfactory for the 4 analytes at 3 concentrations, and both inter\u2010 and intraday reproducibility were satisfactory for T, DHT, and E<jats:sub>2<\/jats:sub>. Measurements of intratesticular T concentrations in a group of 8 untreated adult rats by this method correlated well with measurements of the same samples by radioimmunoassay. As in men, there was considerable rat\u2010to\u2010rat variability in T concentration, despite the fact that the rats were inbred. Although its levels were more than an order of magnitude lower than those of T, DHT was measured reliably in all 8 intratesticular fluid samples. DHT concentration also varied from rat to rat and was highly correlated with T levels. The levels of E<jats:sub>2<\/jats:sub> and 3\u03b1\u2010diol also were measurable. The availability of a sensitive method by which to measure steroids accurately and rapidly in the small volumes of intratesticular fluid obtainable from individual rats will make it possible to examine the effects, over time, of such perturbations as hormone and drug administration and environmental toxicant exposures on the intratesticular hormonal environment of exposed individual males and thereby to begin to understand differences in response between individuals.<\/jats:p>","DOI":"10.2164\/jandrol.111.014977","type":"journal-article","created":{"date-parts":[[2012,7,13]],"date-time":"2012-07-13T09:40:55Z","timestamp":1342172455000},"page":"691-698","source":"Crossref","is-referenced-by-count":7,"title":["Simultaneous Quantification of Steroids in Rat Intratesticular Fluid by HPLC\u2014Isotope Dilution Tandem Mass 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