{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T09:08:50Z","timestamp":1777021730663,"version":"3.51.4"},"reference-count":45,"publisher":"IWA Publishing","issue":"1","content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adenoviruses (AdV) are related to respiratory and gastrointestinal diseases in animals and human beings. Their wide genetic diversity in water bodies and their resistance to environmental conditions allow the use of AdV as a reliable marker for detection of fecal contamination. In this work, the diversity of AdV along Belo Stream \u2013 in the city of Caxias do Sul, Rio Grande do Sul, Brazil \u2013 was evaluated. Samples were compared in both concentrated and unconcentrated forms. The identification of different AdV species was performed by amplifying a partial sequence of the DNA polymerase gene. AdV was detected in 24 out of 55 concentrated samples (43.6%) and the following species were identified: human adenovirus (HAdV) species C (4\/55\u037e 7.2%), D (6\/55\u037e 10.9%), E (2\/55\u037e 3.6%), and F (9\/55\u037e 16.3%). AdV related to other mammalian hosts, such as bovine adenovirus (1\/55, 1.8%) and murine adenovirus (2\/55, 3.6%), have also been identified; 23.6% (13\/55) of the unconcentrated samples were positive, and identified as HAdV species C (6\/55, 10.9%), D (1\/55, 1.8%), and F (6\/55, 10.9%). Results obtained evidenced the presence and the great diversity of AdV, mainly of human origin, circulating in Belo Stream. As expected, the concentration step performed helped to detect AdV in more samples.<\/jats:p>","DOI":"10.2166\/wh.2017.144","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T01:20:10Z","timestamp":1510017610000},"page":"102-111","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":19,"title":["Assessment of diversity of adenovirus DNA polymerase gene in recreational waters facilitated by ultracentrifugal concentration"],"prefix":"10.2166","volume":"16","author":[{"given":"Viviane","family":"Girardi","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, ERS 239 n\u00b0 2755, Novo Hamburgo, RS 93352-000, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meriane","family":"Demoliner","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, ERS 239 n\u00b0 2755, Novo Hamburgo, RS 93352-000, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Rigotto","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, ERS 239 n\u00b0 2755, Novo Hamburgo, RS 93352-000, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vania Elisabete","family":"Schneider","sequence":"first","affiliation":[{"name":"Instituto de Saneamento Ambiental (ISAM), Universidade de Caxias do Sul, Rua Francisco Get\u00falio Vargas, n\u00b0 1130, Caxias do Sul, RS 95070-560, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suelen","family":"Paesi","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Molecular, Instituto de Biotecnologia, Universidade de Caxias do Sul, Rua Francisco Get\u00falio Vargas, n\u00b0 1130, Caxias do Sul, RS 95070-560, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Rosado","family":"Spilki","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, ERS 239 n\u00b0 2755, Novo Hamburgo, RS 93352-000, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","published-online":{"date-parts":[[2017,11,3]]},"reference":[{"key":"2020032615441999300_JWH-D-17-00144C1","volume-title":"Planejamento de amostragem de efluentes l\u00edquidos e corpos receptores \u2013 NBR 9897","author":"ABNT \u2013 Associa\u00e7\u00e3o Brasileira de Normas T\u00e9cnicas","year":"1987"},{"key":"2020032615441999300_JWH-D-17-00144C2","volume-title":"Preserva\u00e7\u00e3o e t\u00e9cnicas de amostragem de efluentes l\u00edquidos e corpos receptores \u2013 NBR 9898","author":"ABNT \u2013 Associa\u00e7\u00e3o Brasileira de Normas T\u00e9cnicas","year":"1987"},{"issue":"4","key":"2020032615441999300_JWH-D-17-00144C3","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/s12560-016-9246-4","article-title":"Quantitative PCR detection and characterisation of human adenovirus, rotavirus and hepatitis A virus in discharged effluents of two wastewater treatment facilities in the Eastern Cape, South Africa","volume":"8","year":"2016","journal-title":"Food Environ. 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