{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T21:54:28Z","timestamp":1683323668798},"reference-count":29,"publisher":"IWA Publishing","issue":"3","content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,6,1]]},"abstract":"<jats:p>Desalination of seawater is becoming an important means to address the increasing scarcity of freshwater resources in the world. Seawater has been used as drinking water in the health, food, and medical fields and various beneficial effects have been suggested, although not confirmed. Given the presence of 63 minerals and trace elements in drinking desalinated seawater (63 DSW), we evaluated their effects on the behavior of tumorigenic and nontumorigenic cells through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and annexin-V-fluorescein isothiocyanate\/propidium iodide staining. Our results showed that cell viability and proliferation in the presence of 63 DSW were significantly greater than in mineral water and in the presence of fetal bovine serum in a dose-dependent manner. Furthermore, 63 DSW showed no toxic effect on murine embryonic fibroblast (NIH-3T3) and murine melanoma (B16-F10) cells. In another assay, we also showed that pre-treatment of non-adherent THP-1 cells with 63 DSW reduces apoptosis incidence, suggesting a protective effect against cell death. We conclude that cell viability and proliferation were improved by the mineral components of 63 DSW and this effect can guide further studies on health effects associated with DSW consumption.<\/jats:p>","DOI":"10.2166\/wh.2017.252","type":"journal-article","created":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T01:25:18Z","timestamp":1486430718000},"page":"360-366","update-policy":"http:\/\/dx.doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":1,"title":["Effects of drinking desalinated seawater on cell viability and proliferation"],"prefix":"10.2166","volume":"15","author":[{"given":"Camila Longhi","family":"Macarr\u00e3o","sequence":"first","affiliation":[{"name":"Universidade Paulista, UNIP, Av. Dr. Bacelar, S\u00e3o Paulo 1212, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Luis Lacerda","family":"Bachi","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias da Atividade F\u00edsica e Esporte, Universidade Cruzeiro do Sul, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Mariano","sequence":"first","affiliation":[{"name":"Departamento de Imunologia, Universidade Federal de S\u00e3o Paulo, UNIFESP, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia Jamli","family":"Abel","sequence":"first","affiliation":[{"name":"Universidade Paulista, UNIP, Av. 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