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Phenotypic screening of 436 morphologically distinct bacterial isolates collected from diverse sites revealed that 106 (\u223c24%) isolates were ESBL positive. Antibiotic profiling showed that 42, 60, 78 and 59% ESBL producing isolates collected from Ghazipur slaughterhouse, Lodhi garden pond, Hauz Khas lake and Jasola wastewater treatment plant, respectively, were multidrug-resistant (MDR). The multiple antibiotic resistance (MAR) index varied from 0.20 to 0.32 among selected locations. The prevalence of ESBL gene variants blaSHV, blaTEM and blaCTX-M were found to be 17.64, 35.29 and 64%, respectively. Furthermore, the analysis of obtained gene sequences showed three variants of blaCTX-M (15, 152 and 205) and two variants of blaTEM (TEM-1 and TEM-116) among ESBL producers. The co-existence of 2\u20133 gene variants was recorded among 48% ESBL positive isolates. New reports from this study include the blaCTX-M gene in Acinetobacter lwoffii, Enterobacter ludwigii, Exiguobacterium mexicanum and Aeromonas caviae. Furthermore, the identification of blaTEM and blaSHV in an environmental isolate of A. caviae is a new report from India.<\/jats:p>","DOI":"10.2166\/wh.2021.085","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T16:13:28Z","timestamp":1622218408000},"page":"592-603","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":35,"title":["Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring <i>bla<\/i>CTX-M, <i>bla<\/i>TEM and <i>bla<\/i>SHV type ESBL genes"],"prefix":"10.2166","volume":"19","author":[{"given":"Asghar","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Insha","family":"Sultan","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aftab Hossain","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Tahir","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firdoos Ahmad","family":"Gogry","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qazi Mohd. 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