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Little information is available on the contamination of environmental media with enteric pathogens in such settlements. We investigated the contamination of Escherichia coli, rotavirus, and Cryptosporidium spp. in water, on kitchenware, and on flies in urban informal settlements of Chawama and Kanyama, Lusaka, Zambia. These environmental media were examined by XM-G agar cultivation for E. coli and specific real-time RT-PCR assays to detect rotavirus and Cryptosporidium spp. E. coli; rotavirus, and Cryptosporidium spp. were detected in samples of household stored drinking water (6 of 10 samples, 3 of 10 samples, and 2 of 10 samples, respectively), cups (10 of 20 samples, 2 of 13 samples, 1 of 13 samples, respectively), and flies (35 of 55 samples, 5 of 17 samples, 1 of 17 samples, respectively). The ranges of rotavirus concentrations in household stored drinking water, on cups, and flies were 2.9 \u00d7 102\u20132.2 \u00d7 105 copies\/L, 1.2 \u00d7 102\u20134.3 \u00d7 102 copies\/cup, and 5.0 \u00d7 101\u20132.0 \u00d7 102 copies\/fly, respectively. These results indicate the contribution of drinking water and kitchenware to enteric pathogen exposure and potential role of flies in microbial transmission.<\/jats:p>","DOI":"10.2166\/wh.2022.276","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T15:22:54Z","timestamp":1654701774000},"page":"1027-1037","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":9,"title":["Detection of <i>Escherichia coli<\/i>, rotavirus, and <i>Cryptosporidium<\/i> spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia"],"prefix":"10.2166","volume":"20","author":[{"given":"Yasuhiro","family":"Asada","sequence":"first","affiliation":[{"name":"a Department of Environmental Health, National Institute of Public Health, Saitama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Li","family":"Chua","sequence":"additional","affiliation":[{"name":"b Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayu","family":"Tsurumi","sequence":"additional","affiliation":[{"name":"b Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro","family":"Yamauchi","sequence":"additional","affiliation":[{"name":"c Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan"},{"name":"d Research Institute for Humanity and Nature, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imasiku","family":"Nyambe","sequence":"additional","affiliation":[{"name":"e Integrated Water Resources Management Centre, c\/o School of Mines, University of Zambia, Lusaka, Zambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7685-7751","authenticated-orcid":false,"given":"Hidenori","family":"Harada","sequence":"additional","affiliation":[{"name":"d Research Institute for Humanity and Nature, Kyoto, Japan"},{"name":"f Graduate School of Asian and African Area Studies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"key":"key-10.2166\/wh.2022.276-1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001644"},{"key":"key-10.2166\/wh.2022.276-2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b06583"},{"key":"key-10.2166\/wh.2022.276-3","doi-asserted-by":"publisher","DOI":"10.1080\/09603123.2018.1453055"},{"key":"key-10.2166\/wh.2022.276-4","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2011-301528"},{"key":"key-10.2166\/wh.2022.276-5","volume-title":"Zambia 2010 Census of Population and Housing","author":"Central Statistics Office","year":"2012"},{"key":"key-10.2166\/wh.2022.276-6","doi-asserted-by":"publisher","DOI":"10.21767\/2573-0282.100064"},{"key":"key-10.2166\/wh.2022.276-7","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01236-15"},{"key":"key-10.2166\/wh.2022.276-8","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0408"},{"key":"key-10.2166\/wh.2022.276-9","doi-asserted-by":"publisher","DOI":"10.2307\/3284666"},{"key":"key-10.2166\/wh.2022.276-10","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990520"},{"key":"key-10.2166\/wh.2022.276-11","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.68.228"},{"key":"key-10.2166\/wh.2022.276-12","volume-title":"Quantitative Microbial Risk Assessment","author":"Haas","year":"1999"},{"key":"key-10.2166\/wh.2022.276-13","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12878"},{"key":"key-10.2166\/wh.2022.276-14","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.3.1033-1039.2002"},{"key":"key-10.2166\/wh.2022.276-15","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12368"},{"key":"key-10.2166\/wh.2022.276-16","doi-asserted-by":"publisher","DOI":"10.1086\/516528"},{"key":"key-10.2166\/wh.2022.276-17","doi-asserted-by":"publisher","DOI":"10.2174\/1874613601610010034"},{"key":"key-10.2166\/wh.2022.276-18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04710.x"},{"key":"key-10.2166\/wh.2022.276-19","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa223"},{"key":"key-10.2166\/wh.2022.276-20","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13003"},{"key":"key-10.2166\/wh.2022.276-21","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(01)00119-1"},{"key":"key-10.2166\/wh.2022.276-22","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-018-9361-5"},{"issue":"1","key":"key-10.2166\/wh.2022.276-23","first-page":"79","article-title":"Contaminated weaning food: a major risk factor for diarrhoea and associated malnutrition","volume":"71","author":"Motarjemi","year":"1993","journal-title":"Bull. 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