{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T13:41:31Z","timestamp":1791466891595,"version":"4.3.4"},"reference-count":47,"publisher":"IWA Publishing","issue":"4","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USDA National Institute of Food and Agriculture, Agricultural and Food Research Initiative Competitive Program, Agriculture Economics and Rural Communities","award":["2018-67017-27631"],"award-info":[{"award-number":["2018-67017-27631"]}]},{"name":"in-kind supplement from the Oregon State University\u2019s Center for Quantitative Life Sciences"}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infections resistant to broad spectrum antibiotics due to the emergence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is of global concern. This study characterizes the resistome (i.e., entire ecology of resistance determinants) of 11 ESBL-producing Escherichia coli isolates collected from eight wastewater treatment utilities across Oregon. Whole genome sequencing was performed to identify the most abundant antibiotic resistance genes including ESBL-associated genes, virulence factors, as well as their sequence types. Moreover, the phenotypes of antibiotic resistance were characterized. ESBL-associated genes (i.e., blaCMY, blaCTX, blaSHV, blaTEM) were found in all but one of the isolates with five isolates carrying two of these genes (four with blaCTX and blaTEM; one with blaCMY and blaTEM). The ampC gene and virulence factors were present in all the E. coli isolates. Across all the isolates, 31 different antibiotic resistance genes were identified. Additionally, all E. coli isolates harbored phenotypic resistance to beta-lactams (penicillins and cephalosporins), while 8 of the 11 isolates carried multidrug resistance phenotypes (resistance to three or more classes of antibiotics). Findings highlight the risks associated with the presence of ESBL-producing E. coli isolates in wastewater systems that have the potential to enter the environment and may pose direct or indirect risks to human health.<\/jats:p>","DOI":"10.2166\/wh.2022.292","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T07:35:00Z","timestamp":1647848100000},"page":"670-679","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":5,"title":["Resistome characterization of extended-spectrum beta-lactamase (ESBL)-producing\n                    <i>Escherichia coli<\/i>\n                    isolated from wastewater treatment utilities in Oregon"],"prefix":"10.2166","volume":"20","author":[{"given":"Maeghan","family":"Easler","sequence":"first","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton","family":"Cheney","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared D.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Oregon State University, 3051 SW Campus Way, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan Khorshidi","family":"Zadeh","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquelynn N.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue Yee","family":"Yiu","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy","family":"Waite-Cusic","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Oregon State University, 3051 SW Campus Way, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler S.","family":"Radniecki","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8391-4673","authenticated-orcid":false,"given":"Tala","family":"Navab-Daneshmand","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological, and Environmental Engineering, Oregon State University, 105 SW 26th St, 116 Johnson Hall, Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"945","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"2022042814534909300_JWH-D-21-00292C1","article-title":"Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany","volume":"10","year":"2020","journal-title":"Scientific Reports"},{"key":"2022042814534909300_JWH-D-21-00292C2","unstructured":"Andrews\n            S.\n          , KruegerF., Segonds-PichonA., BigginsL., KrugerC. & WingettS.2012FastQC. Babraham Bioinformatics."},{"key":"2022042814534909300_JWH-D-21-00292C3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.ijfoodmicro.2015.02.023","article-title":"Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia","volume":"203","year":"2015","journal-title":"International Journal of Food Microbiology"},{"key":"2022042814534909300_JWH-D-21-00292C4","doi-asserted-by":"crossref","first-page":"e0127752","DOI":"10.1371\/journal.pone.0127752","article-title":"Multidrug-resistant and extended spectrum beta-lactamase-producing Escherichia coli in Dutch surface water and wastewater. 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