{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:33:57Z","timestamp":1791495237704,"version":"4.3.4"},"reference-count":46,"publisher":"IWA Publishing","issue":"9","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Children's Hospital of Eastern Ontario","award":["CHAMO"],"award-info":[{"award-number":["CHAMO"]}]},{"DOI":"10.13039\/100020365","name":"Centers for Disease Control and Prevention Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020365","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Province of Ontario's Wastewater Surveillance Initiative"}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p\/>\n                  <jats:p>Recent MPOX viral resurgences have mobilized public health agencies around the world. Recognizing the significant risk of MPOX outbreaks, large-scale human testing, and immunization campaigns have been initiated by local, national, and global public health authorities. Recently, traditional clinical surveillance campaigns for MPOX have been complemented with wastewater surveillance (WWS), building on the effectiveness of existing wastewater programs that were built to monitor SARS-CoV-2 and recently expanded to include influenza and respiratory syncytial virus surveillance in wastewaters. In the present study, we demonstrate and further support the finding that MPOX viral fragments agglomerate in the wastewater solids fraction. Furthermore, this study demonstrates that the current, most commonly used MPOX assays are equally effective at detecting low titers of MPOX viral signal in wastewaters. Finally, MPOX WWS is shown to be more effective at passively tracking outbreaks and\/or resurgences of the disease than clinical testing alone in smaller communities with low human clinical case counts of MPOX.<\/jats:p>","DOI":"10.2166\/wh.2023.145","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T10:25:40Z","timestamp":1694082340000},"page":"1264-1276","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":23,"title":["Rapidly developed, optimized, and applied wastewater surveillance system for real-time monitoring of low-incidence, high-impact MPOX outbreak"],"prefix":"10.2166","volume":"21","author":[{"given":"Chandler H.","family":"Wong","sequence":"first","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Zhang","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa","family":"Eid","sequence":"additional","affiliation":[{"name":"b Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Plaza-Diaz","sequence":"additional","affiliation":[{"name":"b Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pervez","family":"Kabir","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Wan","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Jun","family":"Jia","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Mercier","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ocean","family":"Thakali","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshmi","family":"Pisharody","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Hegazy","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean E.","family":"Stephenson","sequence":"additional","affiliation":[{"name":"b Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanting","family":"Fang","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tram B.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan T.","family":"Ramsay","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Michael","family":"McKay","sequence":"additional","affiliation":[{"name":"c Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON N9B 3P4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryland","family":"Corchis-Scott","sequence":"additional","affiliation":[{"name":"c Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON N9B 3P4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex E.","family":"MacKenzie","sequence":"additional","affiliation":[{"name":"b Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyson E.","family":"Graber","sequence":"additional","affiliation":[{"name":"b Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-7378","authenticated-orcid":false,"given":"Patrick M.","family":"D\u2019 Aoust","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Delatolla","sequence":"additional","affiliation":[{"name":"a Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"945","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"issue":"9","key":"2023092719132641400_JWH-D-23-00145C1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/s41579-022-00776-z","article-title":"Monkeypox virus: A neglected zoonotic pathogen spreads globally","volume":"20","year":"2022","journal-title":"Nature Reviews Microbiology"},{"key":"2023092719132641400_JWH-D-23-00145C2","author":"BCCDC","year":"2022"},{"issue":"9","key":"2023092719132641400_JWH-D-23-00145C3","article-title":"The first two cases of monkeypox infection in MSM in Bahia, Brazil, and viral sequencing","volume":"14","year":"2022","journal-title":"Viruses"},{"issue":"1","key":"2023092719132641400_JWH-D-23-00145C4","first-page":"1","article-title":"Human monkeypox: Current state of knowledge and implications for the future","volume":"1","year":"2016","journal-title":"Tropical Medicine and Infectious Disease"},{"issue":"2","key":"2023092719132641400_JWH-D-23-00145C5","first-page":"1","article-title":"The changing epidemiology of human monkeypox \u2013 A potential threat? 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E.\n          , KansagraS. M., TilsonE. C., MooreZ., FarringtonD. C. & KinsleyK. H.2022To Drive Equity In Monkeypox Response, States Should Learn From COVID-19. Health Affairs Forefront. Available from: https:\/\/www.healthaffairs.org\/do\/10.1377\/forefront.20220923.180528\/full\/ (Accessed April 3, 2023)."},{"key":"2023092719132641400_JWH-D-23-00145C44","year":"2022"},{"key":"2023092719132641400_JWH-D-23-00145C45","article-title":"First detection of monkeypox virus genome in sewersheds in France: The potential of wastewater-Based epidemiology for monitoring emerging disease","year":"2022","journal-title":"Environmental Science and Technology Letters"},{"key":"2023092719132641400_JWH-D-23-00145C46","doi-asserted-by":"crossref","first-page":"5941","DOI":"10.2147\/IDR.S383471","article-title":"Combating stigma and health inequality of monkeypox: Experience from HIV","volume":"15","year":"2022","journal-title":"Infection and Drug Resistance"}],"container-title":["Journal of Water and Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iwaponline.com\/jwh\/article-pdf\/21\/9\/1264\/1296707\/jwh0211264.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iwaponline.com\/jwh\/article-pdf\/21\/9\/1264\/1296707\/jwh0211264.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T15:29:10Z","timestamp":1695828550000},"score":1,"resource":{"primary":{"URL":"https:\/\/iwaponline.com\/jwh\/article\/21\/9\/1264\/97439\/Rapidly-developed-optimized-and-applied-wastewater"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,1]]},"references-count":46,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9,7]]},"published-print":{"date-parts":[[2023,9,1]]}},"URL":"https:\/\/doi.org\/10.2166\/wh.2023.145","relation":{},"ISSN":["1477-8920","1996-7829"],"issn-type":[{"value":"1477-8920","type":"print"},{"value":"1996-7829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,1]]}}}