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More comprehensive surveillance of \u03b2-lactam resistance in E. coli would improve monitoring strategies and control resistance transmission in contaminated environments. This study investigated the prevalence of \u03b2-lactamase genes in E. coli isolated from the Seven Crater Lakes in San Pablo, Laguna, Philippines. Water samples from lakes were collected for the isolation of E. coli (n = 846) and molecular characterization by detecting the presence of the uidA gene. The isolates were then tested for the presence of \u03b2-lactamase genes using PCR. Among the screened genes, blaAmpC was the most dominant (91%). Other \u03b2-lactamase genes such as blaTEM, blaSHV, and blaCTXM were also detected with percentage occurrence of 34, 5, and 1%, respectively. Multiple genes within individual isolates were also observed, wherein blaTEM\/AmpC was the most prevalent gene combination. Moreover, a significant negative correlation between blaAmpC with blaSHV and blaCTXM was depicted in this study. Overall, these findings demonstrate the presence of \u03b2-lactamase genes in E. coli in the Seven Crater Lakes of San Pablo and can be used in developing effective strategies to control antibiotic resistance in environmental waters.<\/jats:p>","DOI":"10.2166\/wh.2023.157","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T10:41:30Z","timestamp":1696329690000},"page":"1518-1529","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":4,"title":["Genotypic detection of <i>\u03b2<\/i>-lactamase-producing <i>Escherichia coli<\/i> isolates obtained from Seven Crater Lakes of San Pablo, Laguna, Philippines"],"prefix":"10.2166","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6945-7074","authenticated-orcid":false,"given":"Diana Rose D.","family":"Mamawal","sequence":"first","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8330-928X","authenticated-orcid":false,"given":"Jonah David V.","family":"Calayo","sequence":"additional","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4769-923X","authenticated-orcid":false,"given":"Kherson P.","family":"Gandola","sequence":"additional","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3723-0403","authenticated-orcid":false,"given":"Mae Ashley G.","family":"Nacario","sequence":"additional","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8655-9379","authenticated-orcid":false,"given":"Mark Raymond A.","family":"Vejano","sequence":"additional","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2218-4798","authenticated-orcid":false,"given":"Laurice Beatrice Raphaelle O.","family":"dela Pe\u00f1a","sequence":"additional","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4659-6132","authenticated-orcid":false,"given":"Windell L.","family":"Rivera","sequence":"additional","affiliation":[{"name":"1 Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"945","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"key":"key-10.2166\/wh.2023.157-1","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2021.085"},{"key":"key-10.2166\/wh.2023.157-2","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2020.146"},{"key":"key-10.2166\/wh.2023.157-3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01916"},{"key":"key-10.2166\/wh.2023.157-4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00338"},{"key":"key-10.2166\/wh.2023.157-5","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.1.77"},{"key":"key-10.2166\/wh.2023.157-6","doi-asserted-by":"publisher","DOI":"10.1007\/s12199-015-0468-0"},{"key":"key-10.2166\/wh.2023.157-7","doi-asserted-by":"publisher","DOI":"10.26757\/pjsb2020a14009"},{"key":"key-10.2166\/wh.2023.157-8","doi-asserted-by":"publisher","DOI":"10.1080\/03601234.2015.1048107"},{"key":"key-10.2166\/wh.2023.157-9","unstructured":"Gehlenborg N. 2019 UpSetR: A More Scalable Alternative to Venn and Euler Diagrams for Visualizing Intersecting Sets. 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