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This study aimed to investigate the presence of these pathogens in infants' drinking water samples and analyzing using water quality determinants in eastern Ethiopia. A molecular (LAMP)-based cross-sectional study design was employed. A total of 410 and 37 water samples were tested from infant point-of-use at household and corresponding water source, respectively, from June 2020 to May, 2021. Cryptosporidium, Shigella, toxin-producing E. coli, and rotavirus were detected in 28.5, 30.0, 26.3, and 32.2%, of water samples tested from infant point-of-use, respectively. About 13.2% of the water samples were positive for all (four) pathogens together. Cryptosporidium, Shigella, toxin-producing E. coli, and rotavirus were detected in 27.0, 32.4, 29.7, and 37.8%, of water samples tested from water sources, respectively. Positive significant correlation was observed between infant point-of-consumption and water sources from which it is drawn toward the presence of each targeted pathogen. Unimproved water source showed a strong significant association with the presence of Cryptosporidium, Shigella and toxin-producing E. coli. Therefore, efforts should be made in development of improved water sources, source protection safety and health education to caretakers of infants.<\/jats:p>","DOI":"10.2166\/wh.2023.201","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T13:34:15Z","timestamp":1702906455000},"page":"1-20","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":4,"title":["Molecular detection of waterborne pathogens in infants' drinking water and their relationship with water quality determinants in eastern Ethiopia: loop-mediated isothermal amplification (LAMP)-based study"],"prefix":"10.2166","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5297-4091","authenticated-orcid":false,"given":"Samuel Mebrahtom","family":"Gebregziabher","sequence":"first","affiliation":[{"name":"a Ethiopian Institute of Water Resources, Addis Ababa University, Addis Ababa, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alemayehu Worku","family":"Yalew","sequence":"additional","affiliation":[{"name":"b School of Public Health, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heven","family":"Sime","sequence":"additional","affiliation":[{"name":"c Bacterial, Parasitic and Zoonotic Diseases Research Directorate, EPHI, Addis Ababa, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adugna","family":"Abera","sequence":"additional","affiliation":[{"name":"c Bacterial, Parasitic and Zoonotic Diseases Research Directorate, EPHI, Addis Ababa, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","published-online":{"date-parts":[[2023,12,18]]},"reference":[{"key":"key-10.2166\/wh.2023.201-1","doi-asserted-by":"publisher","DOI":"10.5604\/12321966.1226849"},{"issue":"1","key":"key-10.2166\/wh.2023.201-2","first-page":"1","article-title":"Bacteriological quality and occurrence of cryptosporidium and giardia from sources to household water samples of the rural communities of Dire Dawa Administrative Council, Eastern Ethiopia","volume":"1","author":"Amenu","year":"2013","journal-title":"International Journal of Bacteriology Research"},{"issue":"2","key":"key-10.2166\/wh.2023.201-3","first-page":"1048","article-title":"Environmental aspects of cryptosporidium","volume":"3","author":"Armon","year":"2016","journal-title":"Journal of Veterinary Medicine and Research"},{"issue":"3","key":"key-10.2166\/wh.2023.201-4","first-page":"103","article-title":"High enteric bacterial contamination of drinking water in Jigjiga city, Eastern Ethiopia","volume":"30","author":"Asfaw","year":"2016","journal-title":"Ethiopian Journal of Health Development"},{"key":"key-10.2166\/wh.2023.201-5","doi-asserted-by":"publisher","DOI":"10.1007\/s12403-012-0065-z"},{"key":"key-10.2166\/wh.2023.201-6","doi-asserted-by":"publisher","DOI":"10.21608\/mjcu.2019.54864"},{"key":"key-10.2166\/wh.2023.201-7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001644"},{"key":"key-10.2166\/wh.2023.201-8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009543"},{"key":"key-10.2166\/wh.2023.201-9","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2022.230"},{"key":"key-10.2166\/wh.2023.201-10","doi-asserted-by":"publisher","DOI":"10.1039\/C9AY02246E"},{"key":"key-10.2166\/wh.2023.201-11","volume-title":"What is an Oligo- or Oligonucleotide?","author":"Bio-Synthesis Inc.","year":"2014"},{"key":"key-10.2166\/wh.2023.201-12","doi-asserted-by":"publisher","DOI":"10.21010\/ajid.v15i1.7"},{"key":"key-10.2166\/wh.2023.201-13","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2008.04.006"},{"key":"key-10.2166\/wh.2023.201-14","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-6399-3"},{"key":"key-10.2166\/wh.2023.201-15","article-title":"Shiga toxin-producing Escherichia coli isolation and characterization in drinking water","author":"Caroline","year":"2018"},{"key":"key-10.2166\/wh.2023.201-16","unstructured":"Centers for Disease Control and Prevention (CDC). 2011 The Safe Water System: Safe Storage of Drinking Water. 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