{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T12:57:28Z","timestamp":1771073848009,"version":"3.50.1"},"reference-count":23,"publisher":"IWA Publishing","issue":"10","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:p>Cryptosporidium spp. are protozoan parasites of significant health importance found in environmental waters globally. Four commercially available Cryptosporidium-specific immunomagnetic separation (IMS) kits used in various water sample matrices were analysed and compared. Beads were characterised by flow cytometry and tested for the recovery efficiencies for oocysts spiked into different matrices: river water sediment, clay sample, and filter backwash sample. Results showed that Dynabeads\u2122 Cryptosporidium and Waterborne Crypto-Grab\u2122 kits contained immunoglobulin IgM antibody-coated beads. In contrast, the BioPoint CryptoBead and the TCS Isolate kits contained immunoglobulin IgG antibody-coated beads. BioPoint CryptoBead was significantly coated with more antibodies and were able to capture oocysts more rapidly compared to the other beads. Recovery efficiencies of Dynabeads\u2122, TCS Isolate\u00ae beads, and BioPoint CryptoBead ranged from 55 to 93% when tested against different sample matrices, with BioPoint CryptoBead resulting in the highest at 93% in reagent-grade water and Dynabeads\u2122 at 55%, the lowest against clay samples. The Waterborne beads did not perform well on any samples, with recovery efficiencies ranging from 0 to 8%. Fluorescence microscopy analyses showed that both the IMS method and the sample matrix processed affect the quality of the membranes, with the cleanest samples for microscopy examination observed from BioPoint CryptoBead.<\/jats:p>","DOI":"10.2166\/wh.2023.217","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T16:20:56Z","timestamp":1695918056000},"page":"1580-1590","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of four commercial immunomagnetic separation kits for the detection of <i>Cryptosporidium<\/i>"],"prefix":"10.2166","volume":"21","author":[{"given":"Jeanne Claudeen","family":"Arona","sequence":"first","affiliation":[{"name":"1 Graduate School of BioMedical Engineering, University of New South Wales, Sydney 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy J.","family":"Hall","sequence":"additional","affiliation":[{"name":"1 Graduate School of BioMedical Engineering, University of New South Wales, Sydney 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flyn","family":"Mckinnirey","sequence":"additional","affiliation":[{"name":"1 Graduate School of BioMedical Engineering, University of New South Wales, Sydney 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Deng","sequence":"additional","affiliation":[{"name":"1 Graduate School of BioMedical Engineering, University of New South Wales, Sydney 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"key-10.2166\/wh.2023.217-1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13132280"},{"key":"key-10.2166\/wh.2023.217-2","doi-asserted-by":"publisher","DOI":"10.1080\/09593330.2017.1400113"},{"key":"key-10.2166\/wh.2023.217-3","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06999-4"},{"key":"key-10.2166\/wh.2023.217-4","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2022.e00160"},{"issue":"1","key":"key-10.2166\/wh.2023.217-5","first-page":"144","article-title":"Immunomagnetic separation study applied to detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in water samples","volume":"16","author":"de Oliveira Pinto","year":"2016","journal-title":"Water Science and Technology: Water Supply"},{"key":"key-10.2166\/wh.2023.217-6","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-018-7057-9"},{"key":"key-10.2166\/wh.2023.217-7","doi-asserted-by":"crossref","DOI":"10.1016\/j.humimm.2023.06.006","article-title":"Epitopes, paratopes, and other topes 30 years on: understanding what we are talking about","author":"Greenspan","year":"2023","journal-title":"Human Immunology"},{"key":"key-10.2166\/wh.2023.217-8","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2020.515"},{"key":"key-10.2166\/wh.2023.217-9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-4581.2001.tb00230.x"},{"key":"key-10.2166\/wh.2023.217-10","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(76)80503-3"},{"key":"key-10.2166\/wh.2023.217-11","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2021.e00131"},{"key":"key-10.2166\/wh.2023.217-12","doi-asserted-by":"publisher","DOI":"10.1080\/01919512.2021.1960148"},{"key":"key-10.2166\/wh.2023.217-13","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-64.11.1799"},{"key":"key-10.2166\/wh.2023.217-14","doi-asserted-by":"publisher","DOI":"10.3390\/ani11113307"},{"key":"key-10.2166\/wh.2023.217-15","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00338-7"},{"key":"key-10.2166\/wh.2023.217-16","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.05.014"},{"key":"key-10.2166\/wh.2023.217-17","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9071463"},{"key":"key-10.2166\/wh.2023.217-18","volume-title":"Cryptosporidium and Giardia in Water by Filtration\/IMS\/FA","author":"U.S. EPA. 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