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By using the Pensacola Bay System as a case study, this study examines the underappreciated susceptibility of developed subtropical coastal ecosystems such as the Pensacola Bay System to neglected zoonotic pathogens such as Leptospira. We analyzed 132 water samples collected over 12 months from 44 distinct locations with high levels of Escherichia coli (&amp;gt;410 most probable number\/100 mL). Fecal indicator bacteria (FIB) concentrations were assessed using IDEXX Colilert-18 and Enterolert-18, and an analysis of water physiochemical characteristics and rainfall intensity was conducted. The LipL32 gene was used as a quantitative polymerase chain reaction (qPCR) indicator to identify the distribution of Leptospira interrogans. The results revealed 12 instances of the presence of L. interrogans at sites with high FIB over various land cover and aquatic ecosystem types. Independent of specific rainfall events, a seasonal relationship between precipitation and elevated rates of fecal bacteria and leptospirosis was found. These findings highlight qPCR's utility in identifying pathogens in aquatic environments and the widespread conditions where it can be found in natural and developed areas.<\/jats:p>","DOI":"10.2166\/wh.2024.038","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T09:37:26Z","timestamp":1712050646000},"page":"923-938","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":7,"title":["Spatiotemporal assessment of pathogenic\n                    <i>Leptospira<\/i>\n                    in subtropical coastal watersheds"],"prefix":"10.2166","volume":"22","author":[{"given":"Ronell","family":"Bridgemohan","sequence":"first","affiliation":[{"name":"a Soil, Water, and Ecosystem Sciences Department, University of Florida IFAS West Florida Research and Education Center, Milton, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Deitch","sequence":"additional","affiliation":[{"name":"a Soil, Water, and Ecosystem Sciences Department, University of Florida IFAS West Florida Research and Education Center, Milton, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Harmon","sequence":"additional","affiliation":[{"name":"a Soil, Water, and Ecosystem Sciences Department, University of Florida IFAS West Florida Research and Education Center, Milton, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt R.","family":"Whiles","sequence":"additional","affiliation":[{"name":"b Soil, Water, and Ecosystem Sciences Department 2181 McCarty Hall, University of Florida, P.O. 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