{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T06:10:01Z","timestamp":1794550201117,"version":"4.9.1"},"reference-count":26,"publisher":"IWA Publishing","issue":"9","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020473","name":"Badan Riset dan Inovasi Nasional","doi-asserted-by":"publisher","award":["164\/IV\/KS\/11\/2023"],"award-info":[{"award-number":["164\/IV\/KS\/11\/2023"]}],"id":[{"id":"10.13039\/100020473","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p\/>\n               <jats:p>The detection of bacterial contamination in drinking water is essential for monitoring the spread of foodborne diseases. We developed a simple, portable, and low-cost method of mini most probable number (mini MPN) to semi-enumerate bacterial suspension in water as a drinking water analogue. In this study, there is no significant difference between mini MPN and the standard method, technique plate count (TPC), at 10 and 100 CFU\/ml Klebsiella pneumoniae suspension with a P-value of 0.28. For the ease-of-use aspect of this method, we tested several variables to prove it can be mass-applied in society. The usage of a sterile-plastic pipette, sample inoculation conducted in a biosafety cabinet (BSC), the usage of a 3-month storage medium, and incubation temperature conducted at room temperature compared to aseptic standard laboratory technique showed P-value &amp;gt; 0.05. In a trial for this method, we used commercialized drinking water for bacterial enumeration and characterization. We found multi-drug resistant (MDR) Ralstonia insidiosa which was resistant to at least four antimicrobial classes, including aminoglycosides, penicillins, cephalosporin, and carbapenem. Vitek 2 Compact was used for bacterial identification and antimicrobial susceptibility testing. A virulence test in Omphisa fuscidentalis larvae showed R. insidiosa strain D had a low virulence.<\/jats:p>","DOI":"10.2166\/wh.2024.086","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T13:17:07Z","timestamp":1723814227000},"page":"1618-1627","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":3,"title":["Application of miniaturized most probable number method for bacterial detection in water samples: detection of multi-drug-resistant <i>Ralstonia insidiosa<\/i> in drinking water"],"prefix":"10.2166","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9907-8750","authenticated-orcid":false,"given":"Noor Andryan","family":"Ilsan","sequence":"first","affiliation":[{"name":"a Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, West Java Province, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti","family":"Nurfajriah","sequence":"additional","affiliation":[{"name":"a Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, West Java Province, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maulin","family":"Inggraini","sequence":"additional","affiliation":[{"name":"a Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, West Java Province, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni Putu Ratna Ayu","family":"Krishanti","sequence":"additional","affiliation":[{"name":"b Research Center for Applied Zoology, National Research and Innovation Agency, Soekarno Science and Techno Park, Jl. Raya Bogor KM 46, Cibinong, Bogor, West Java Province 16911, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melda","family":"Yunita","sequence":"additional","affiliation":[{"name":"c Department of Medical Education, Faculty of Medicine, Pattimura University, Ambon, Maluku, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Sipriyadi","sequence":"additional","affiliation":[{"name":"d Department of Biology, Faculty of Mathematics and Natural Sciences, Bengkulu University, Bengkulu City, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Anindita","sequence":"additional","affiliation":[{"name":"e Department of Pharmacy, STIKes Mitra Keluarga, Bekasi City, West Java Province, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"f Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 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