{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:30:14Z","timestamp":1790047814200,"version":"4.0.1"},"reference-count":83,"publisher":"IWA Publishing","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:p>The objective of this study was to assess the occurrence and seasonal frequency of human adenovirus (HAdV), human polyomavirus (HPyV), and human papillomavirus (HPV) in urban sewage. The detection of these viruses was carried out by polymerase chain reaction (PCR), and then the viral concentrations in the positive samples were quantified by quantitative PCR (qPCR). Additionally, HAdV and HPyV genotyping was also performed by PCR. A total of 38\/60 (63.3%) positive samples were found. HAdV was the most prevalent virus (26\/60; 43.3%), followed by HPyV (21\/60; 35%) and HPV (21\/60; 35%). The viral concentrations ranged from 3.56 \u00d7 102 to 7.55 \u00d7 107 genome copies\/L. The most common dual viral agents was found between HAdV and HPyV, in eight samples (8\/38, 21%). HAdV types 40 and 41 as well as HPyV types JC and BK were identified, with HAdV-40 and HPyV JC being the most prevalent types. Furthermore, the detection rates of HAdV, HPyV, and HPV were higher during the winter season than the other seasons. The high prevalence of HAdV and HPyV supports their suitability as viral indicators of sewage contamination. Furthermore, this study demonstrates the advantages of environmental surveillance as a tool to elucidate the community-circulating viruses.<\/jats:p>","DOI":"10.2166\/wh.2024.322","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T17:04:50Z","timestamp":1707325490000},"page":"401-413","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":20,"title":["Detection and quantification of adenovirus, polyomavirus, and papillomavirus in urban sewage"],"prefix":"10.2166","volume":"22","author":[{"given":"Mohamed N. F.","family":"Shaheen","sequence":"first","affiliation":[{"name":"1 Environmental Virology Laboratory, Department of Water Pollution Research, Environment and Climate Change Research Institute, National Research Center, 12622 Dokki, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nehal","family":"Ahmed","sequence":"additional","affiliation":[{"name":"1 Environmental Virology Laboratory, Department of Water Pollution Research, Environment and Climate Change Research Institute, National Research Center, 12622 Dokki, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kareem","family":"Rady Badr","sequence":"additional","affiliation":[{"name":"1 Environmental Virology Laboratory, Department of Water Pollution Research, Environment and Climate Change Research Institute, National Research Center, 12622 Dokki, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmahdy Mohamed","family":"Elmahdy","sequence":"additional","affiliation":[{"name":"1 Environmental Virology Laboratory, Department of Water Pollution Research, Environment and Climate Change Research Institute, National Research Center, 12622 Dokki, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"945","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"2024022915555429400_JWH-D-23-00322C1","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/s12560-016-9246-4","article-title":"Quantitative PCR detection and characterisation of human adenovirus, rotavirus and hepatitis A virus in discharged effluents of two wastewater treatment facilities in the Eastern Cape, South Africa","volume":"8","year":"2016","journal-title":"Food and Environmental Virology"},{"issue":"2","key":"2024022915555429400_JWH-D-23-00322C3","doi-asserted-by":"crossref","first-page":"29","DOI":"10.21608\/ejabf.2019.29237","article-title":"Prevalence of human polyomavirus 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