{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:39:12Z","timestamp":1740145152701,"version":"3.37.3"},"reference-count":19,"publisher":"IWA Publishing","issue":"4","license":[{"start":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T00:00:00Z","timestamp":1708560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005802","name":"Autoritatea National\u0103 pentru Cercetare Stiintific\u0103","doi-asserted-by":"publisher","award":["National Authority of Scientific  Research and Innovation, Romania, Core Project PN 19 14 02 07."],"award-info":[{"award-number":["National Authority of Scientific  Research and Innovation, Romania, Core Project PN 19 14 02 07."]}],"id":[{"id":"10.13039\/501100005802","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The measurement of the enterovirus and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in sewage water is relevant in the early detection of the introduction or disappearance of these viruses in the ecosystem. We evaluated the co-circulation of the enteroviruses and SARS-CoV-2 in 81 sewage water samples collected between September 2021 and April 2023 from different regions of north and southeast Romania, at the border with Ukraine. We used, for the molecular detection of the pathogens, the multiplex real-time polymerase chain reaction (PCR) assay produced for respiratory samples and the Respiratory 2.1 Plus panel Biofire Film array. The isolation of enteroviruses was performed on cell culture lines, in accordance with the World Health Organization (WHO) recommendations. By molecular investigations, we detected the SARS-CoV-2 in 22 (27%) samples, and the human rhinovirus\/enterovirus in 64 (79%) samples. By isolation on cell culture lines, 27 samples (33,33%) were positive for non-polio enteroviruses, and no poliovirus strains were isolated, proving the maintenance of the polio-free status in Romania. In an emergency situation, the molecular detection of the pathogens in sewage water using a PCR system integrating sample preparation, amplification, detection, and analysis in 1 h could be implemented.<\/jats:p>","DOI":"10.2166\/wh.2024.327","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T15:19:19Z","timestamp":1708615159000},"page":"717-720","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":0,"title":["Poliomyelitis and COVID-19: a repeated history"],"prefix":"10.2166","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4929-6493","authenticated-orcid":false,"given":"Baicus","family":"Anda","sequence":"first","affiliation":[{"name":"a Enteric Viral Infections, Laboratory \u2018Cantacuzino\u2019 Medico Military National Institute of Research and Development, 050096 Bucharest, Romania"},{"name":"b Department of Microbiology III \u2013 Emergency University Hospital Bucharest, Faculty of Medicine, \u2018Carol Davila\u2019 University of Medicine and Pharmacy, 050098 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","published-online":{"date-parts":[[2024,2,22]]},"reference":[{"key":"key-10.2166\/wh.2024.327-1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-020-04772-7"},{"key":"key-10.2166\/wh.2024.327-2","doi-asserted-by":"publisher","DOI":"10.3390\/v13050844"},{"key":"key-10.2166\/wh.2024.327-3","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.144105"},{"key":"key-10.2166\/wh.2024.327-4","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.139654"},{"key":"key-10.2166\/wh.2024.327-5","unstructured":"Government of Canada 2023 Polio (Poliomyelitis): Wastewater Surveillance. Available from: https:\/\/www.canada.ca\/en\/public-health\/services\/diseases\/poliomyelitis-polio\/wastewater-surveillance.html (accessed 7 January 2024)."},{"key":"key-10.2166\/wh.2024.327-6","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.02.054"},{"key":"key-10.2166\/wh.2024.327-7","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2313"},{"key":"key-10.2166\/wh.2024.327-8","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01885-2"},{"issue":"24","key":"key-10.2166\/wh.2024.327-9","first-page":"2100","article-title":"Investigation of airport sewage to detect importation of poliovirus. Poland, 2017 to 2020","volume":"27","author":"Krzysztoszek","year":"2022","journal-title":"Euro Surveillance"},{"key":"key-10.2166\/wh.2024.327-10","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7133e2"},{"key":"key-10.2166\/wh.2024.327-11","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.0c00357"},{"key":"key-10.2166\/wh.2024.327-12","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a119132"},{"key":"key-10.2166\/wh.2024.327-13","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00103-22"},{"key":"key-10.2166\/wh.2024.327-14","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7144e2"},{"key":"key-10.2166\/wh.2024.327-15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2023.04.170"},{"key":"key-10.2166\/wh.2024.327-16","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad355"},{"volume-title":"Guidelines for Environmental Surveillance of Poliovirus Circulation","year":"2003","author":"WHO","key":"key-10.2166\/wh.2024.327-17"},{"key":"key-10.2166\/wh.2024.327-18","volume-title":"Polio Laboratory Manual","author":"WHO","year":"2004","edition":"4"},{"key":"key-10.2166\/wh.2024.327-19","unstructured":"WHO 2022 Circulating Vaccine-Derived Poliovirus Type 3 \u2013 Israel. 2022. World Health Organization. Available from: https:\/\/www.who.int\/europe\/news\/item\/10-03-2022-circulating-vaccine-derived-poliovirus-confirmed-in-israel-and-the-occupied-palestinian-territory (accessed 7 January 2024)."}],"container-title":["Journal of Water and Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iwaponline.com\/jwh\/article-pdf\/22\/4\/717\/1406492\/jwh0220717.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iwaponline.com\/jwh\/article-pdf\/22\/4\/717\/1406492\/jwh0220717.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,28]],"date-time":"2024-04-28T11:06:31Z","timestamp":1714302391000},"score":1,"resource":{"primary":{"URL":"https:\/\/iwaponline.com\/jwh\/article\/22\/4\/717\/100565\/Poliomyelitis-and-COVID-19-a-repeated-history"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,22]]},"references-count":19,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,2,22]]},"published-print":{"date-parts":[[2024,4,1]]}},"URL":"https:\/\/doi.org\/10.2166\/wh.2024.327","relation":{},"ISSN":["1477-8920","1996-7829"],"issn-type":[{"type":"print","value":"1477-8920"},{"type":"electronic","value":"1996-7829"}],"subject":[],"published":{"date-parts":[[2024,2,22]]}}}