{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T10:10:39Z","timestamp":1710583839870},"reference-count":0,"publisher":"IWA Publishing","issue":"6","content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,9,1]]},"abstract":"<jats:p>Appropriate preservation of a range of water types prior to analysis for endotoxin was investigated, including sample storage and addition of sodium thiosulphate. Biologically active endotoxin in water samples was assayed using a chromogenic Limulus Amoebocyte Lysate (LAL) assay. Statistical analysis of measured mean endotoxin levels obtained for samples with and without sodium thiosulphate showed no significant difference in results. There was a 44% mean decline in the concentration of detectable endotoxin in water samples stored at \u221280\u00b0C for 4 weeks compared with samples stored at 4\u00b0C and analysed within 24 hours. Freezing of water samples at \u221280\u00b0C in pyrogen-free containers for 4 weeks or longer, then thawing may lead to considerable endotoxin loss; however the addition of sodium thiosulphate to water samples interferes minimally with the LAL assay. These results provide methodological information that can be used to assist researchers in future water endotoxin monitoring studies. The validation and standardisation of water sample preservation protocols are necessary, given the likely increase in the quantification of endotoxin levels in a variety of water sources and the use of such results for health effect determinations.<\/jats:p>","DOI":"10.2166\/wst.2009.494","type":"journal-article","created":{"date-parts":[[2009,9,22]],"date-time":"2009-09-22T09:46:00Z","timestamp":1253612760000},"page":"1615-1619","update-policy":"http:\/\/dx.doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":6,"title":["Influence of sample preservation on endotoxin measurement in water"],"prefix":"10.2166","volume":"60","author":[{"given":"J.","family":"O'Toole","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Preventive Medicine, Monash University Australia, The Alfred, Commercial Rd, Melbourne 3004, Australia E-mail: karin.leder@med.monash.edu.au; martha.sinclair@med.monash.edu.au"},{"name":"CRC for Water Quality and Treatment, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sinclair","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Preventive Medicine, Monash University Australia, The Alfred, Commercial Rd, Melbourne 3004, Australia E-mail: karin.leder@med.monash.edu.au; martha.sinclair@med.monash.edu.au"},{"name":"CRC for Water Quality and Treatment, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Jeavons","sequence":"first","affiliation":[{"name":"Department of Health Science, Monash University, McMahons Road Frankston, Victoria 3199, Australia E-mail: tom.jeavons@med.monash.edu.au"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Leder","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Preventive Medicine, Monash University Australia, The Alfred, Commercial Rd, Melbourne 3004, Australia E-mail: karin.leder@med.monash.edu.au; martha.sinclair@med.monash.edu.au"},{"name":"CRC for Water Quality and Treatment, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","container-title":["Water Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/iwaponline.com\/wst\/article-pdf\/60\/6\/1615\/447580\/1615.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/iwaponline.com\/wst\/article-pdf\/60\/6\/1615\/447580\/1615.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T08:24:08Z","timestamp":1585383848000},"score":1,"resource":{"primary":{"URL":"https:\/\/iwaponline.com\/wst\/article\/60\/6\/1615\/15903\/Influence-of-sample-preservation-on-endotoxin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,9,1]]}},"URL":"https:\/\/doi.org\/10.2166\/wst.2009.494","relation":{},"ISSN":["0273-1223","1996-9732"],"issn-type":[{"value":"0273-1223","type":"print"},{"value":"1996-9732","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2009,9,1]]},"published":{"date-parts":[[2009,9,1]]}}}