{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T21:00:25Z","timestamp":1761339625468},"reference-count":36,"publisher":"IWA Publishing","issue":"11","content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,12,14]]},"abstract":"<jats:p>To access better removal of nutrients with algae-based techniques, a dominant alga from real municipal wastewater was identified and its capacity in removing low concentrations of nitrogen (NH+4 or NO\u22123) and phosphorus (PO3\u22124) was evaluated. Results showed that Oedogonium brevicingulatum, a filamentous green alga, was confirmed as the dominant alga in the secondary effluent of a municipal wastewater treatment plant by polymerase chain reaction-denaturing gradient gel electrophoresis. Low concentrations of NH+4 or NO\u22123 (\u22645 mg N L\u22121) and PO3\u22124 (\u22640.5 mg P L\u22121) were 100% removed by the algae in a 7-d test. The maximum nutrient removal rate (Vmax) and the half-saturation constant (Km) for NH+4 (10.03 \u00b1 0.95 mg g\u22121d\u22121 and 0.19 \u00b1 0.03 mg L\u22121) and NO\u22123 (8.43 \u00b1 0.21 mg g\u22121 d\u22121 and 0.27 \u00b1 0.11 mg L\u22121) indicated the uptake capability for NH+4 is higher than that for NO\u22123. Meanwhile, it showed higher affinity for PO3\u22124 (Vmax: 1.42 \u00b1 0.02 mg g\u22121 d\u22121; Km: 0.02 \u00b1 0.00 mg L\u22121) with NH+4 as nitrogen source than that (Vmax: 1.24 \u00b1 0.15 mg g\u22121 d\u22121; Km: 0.06 \u00b1 0.03 mg L\u22121) with NO\u22123 as nitrogen source. Moreover, nutrient removal efficiencies were observed steady when nitrogen\/phosphorus ratio ranged from 5:1 to 20:1. These results suggest that the dominant algae from municipal wastewater have potentials to be applied in nutrient removal.<\/jats:p>","DOI":"10.2166\/wst.2016.437","type":"journal-article","created":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:29:00Z","timestamp":1475368140000},"page":"2727-2735","update-policy":"http:\/\/dx.doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":8,"title":["Identification and evaluation of a dominant alga from municipal wastewater in removal of nutrients"],"prefix":"10.2166","volume":"74","author":[{"given":"Yixuan","family":"Yang","sequence":"first","affiliation":[{"name":"School of Environment and Energy, South China University of Technology, Guangzhou 510006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan 411105, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Su","sequence":"first","affiliation":[{"name":"Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan 411105, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Yin","sequence":"first","affiliation":[{"name":"School of Environment and Energy, South China University of Technology, Guangzhou 510006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan 411105, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"945","published-online":{"date-parts":[[2016,10,1]]},"reference":[{"key":"2020032801403549600_WST-EM16416R2C1","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1080\/15226514.2012.735291","article-title":"Photobiotreatment: influence of nitrogen and phosphorus ratio in wastewater on growth kinetics of Scenedesmus obliquus","volume":"15","author":"Arbib","year":"2013","journal-title":"International Journal of Phytoremediation"},{"key":"2020032801403549600_WST-EM16416R2C2","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ecoleng.2006.04.003","article-title":"Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae","volume":"28","author":"Aslan","year":"2006","journal-title":"Ecological Engineering"},{"key":"2020032801403549600_WST-EM16416R2C3","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.biortech.2015.05.086","article-title":"Use of PCR-DGGE based molecular methods to assessment of microbial diversity during anaerobic treatment of antibiotic combinations","volume":"192","author":"Aydin","year":"2015","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C4","doi-asserted-by":"crossref","first-page":"5925","DOI":"10.1016\/j.watres.2011.08.044","article-title":"Nitrogen and phosphorus removal from municipal wastewater effluent using microalgal biofilms","volume":"45","author":"Boelee","year":"2011","journal-title":"Water Research"},{"key":"2020032801403549600_WST-EM16416R2C5","first-page":"339","article-title":"Coagulation performance and floc properties of Microcystis aeruginosa in the presence of humic acid","volume":"15","author":"Cheng","year":"2015","journal-title":"Water Science & Technology: Water Supply"},{"key":"2020032801403549600_WST-EM16416R2C6","doi-asserted-by":"crossref","first-page":"141","DOI":"10.4155\/bfs.13.78","article-title":"Ecobiological aspects of algae cultivation in waste wastewaters for recycling of nutrients and biofuel applications","volume":"5","author":"Chinnasamy","year":"2014","journal-title":"Biofuels"},{"key":"2020032801403549600_WST-EM16416R2C7","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0925-8574(95)00056-9","article-title":"A controlled stream mesocosm for tertiary treatment of sewage","volume":"6","author":"Craggs","year":"1996","journal-title":"Ecologic Engineering"},{"key":"2020032801403549600_WST-EM16416R2C8","doi-asserted-by":"crossref","first-page":"133","DOI":"10.2166\/wst.1996.0272","article-title":"Influence of environmental parameters on biomass production and nutrient removal in a high rate algal pond operated by continuous culture","volume":"34","author":"Cromar","year":"1996","journal-title":"Water Science Technology"},{"key":"2020032801403549600_WST-EM16416R2C9","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1016\/j.biortech.2009.09.043","article-title":"Immobilized microalgae for removing pollutants: review of practical aspects","volume":"101","author":"de-Bashan","year":"2010","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C10","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.watres.2003.09.022","article-title":"Microalgae growth-promoting bacteria as \u2018helpers\u2019 for microalgae: a novel approach for removing ammonium and phosphorus from municipal wastewater","volume":"38","author":"de-Bashan","year":"2004","journal-title":"Water Research"},{"key":"2020032801403549600_WST-EM16416R2C11a","doi-asserted-by":"crossref","first-page":"5355","DOI":"10.1016\/j.watres.2012.07.023","article-title":"Evaluation of DNA extraction methods for freshwater eukaryotic microalgae","volume":"46","author":"Eland","year":"2012","journal-title":"Water Research"},{"key":"2020032801403549600_WST-EM16416R2C11","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1016\/j.biortech.2005.07.019","article-title":"Long term diurnal variations in contaminant removal in high rate ponds treating urban wastewater","volume":"97","author":"Garcia","year":"2006","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C12","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.jhazmat.2010.02.022","article-title":"Algal-based immobilization process to treat the effluent from a secondary wastewater treatment plant (WWTP)","volume":"178","author":"He","year":"2010","journal-title":"Journal of Hazardous Material"},{"key":"2020032801403549600_WST-EM16416R2C13","first-page":"373","volume-title":"Freshwater Algae of China [M]","author":"Hu","year":"1980"},{"key":"2020032801403549600_WST-EM16416R2C14","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1023\/A:1004008721373","article-title":"Competition between the green alga Scenedesmus and the cyanobacterium Synechococcus under different modes of inorganic nitrogen supply","volume":"435","author":"Hyenstrand","year":"2000","journal-title":"Hydrobiologia"},{"issue":"2","key":"2020032801403549600_WST-EM16416R2C15","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.mimet.2003.12.006","article-title":"A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis","volume":"57","author":"Janse","year":"2004","journal-title":"Journal of Microbiological Methods"},{"key":"2020032801403549600_WST-EM16416R2C16","first-page":"57","article-title":"New Oedogonia collected in China. II","volume":"20","author":"Jao","year":"1935","journal-title":"Papers of the Michigan Academy of Sciences, Arts and Letters"},{"key":"2020032801403549600_WST-EM16416R2C17","doi-asserted-by":"crossref","first-page":"5494","DOI":"10.1016\/j.biortech.2010.02.016","article-title":"Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp","volume":"101","author":"Li","year":"2010","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C18","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.ecoleng.2009.11.003","article-title":"Growth and nutrient removal properties of a freshwater microalga Scenedesmus sp. LX1 under different kinds of nitrogen sources","volume":"36","author":"Li","year":"2010","journal-title":"Ecological Engineering"},{"key":"2020032801403549600_WST-EM16416R2C19","doi-asserted-by":"crossref","first-page":"5138","DOI":"10.1016\/j.biortech.2011.01.091","article-title":"Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production","volume":"102","author":"Li","year":"2011","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C20","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/j.chemosphere.2013.05.014","article-title":"Efficiency assessment and pH effect in removing nitrogen and phosphorus by algae-bacteria combined system of Chlorella vulgaris and Bacillus licheniformis","volume":"92","author":"Liang","year":"2013","journal-title":"Chemosphere"},{"key":"2020032801403549600_WST-EM16416R2C21","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.biortech.2014.12.028","article-title":"Differences in nutrient uptake capacity of the benthic filamentous alga Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N\/P conditions","volume":"179","author":"Liu","year":"2015","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C22","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.ejop.2007.04.003","article-title":"Classification of the peritrich ciliate Opisthonecta matiensis (Martin-Cereceda et al. 1999) as Telotrochidium matiense nov. comb., based on new observations and SSU rDNA phylogeny","volume":"43","author":"Martin-Cereceda","year":"2007","journal-title":"European Journal of Protistology"},{"key":"2020032801403549600_WST-EM16416R2C23","doi-asserted-by":"crossref","first-page":"996","DOI":"10.4319\/lo.1977.22.6.0996","article-title":"Nitrogenous nutrition of the plankton, in the Chesapeake Bay. 1. Nutrient availability and phytoplankton preferences","volume":"22","author":"McCarthy","year":"1977","journal-title":"Limnology & Oceanography"},{"key":"2020032801403549600_WST-EM16416R2C24","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.resmic.2014.12.010","article-title":"Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating intracellular ammonium in Chlorella vulgaris","volume":"166","author":"Mezaa","year":"2015","journal-title":"Research in Microbiology"},{"key":"2020032801403549600_WST-EM16416R2C25","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1007\/s10811-013-9982-x","article-title":"Evaluation of microalgal consortia for treatment of primary treated sewage effluent and biomass production","volume":"25","author":"Renuka","year":"2013","journal-title":"Journal of Applied Phycology"},{"key":"2020032801403549600_WST-EM16416R2C26","doi-asserted-by":"crossref","first-page":"e81631","DOI":"10.1371\/journal.pone.0081631","article-title":"The effect of CO2 on algal growth in industrial waste water for bioenergy and bioremediation applications","volume":"8","author":"Roberts","year":"2013","journal-title":"PLoS One"},{"key":"2020032801403549600_WST-EM16416R2C27","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.wse.2015.11.003","article-title":"Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting","volume":"8","author":"Ruan","year":"2015","journal-title":"Water Science & Engineering"},{"key":"2020032801403549600_WST-EM16416R2C28","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.ympev.2004.12.018","article-title":"Phylogeny of Tubificidae (Annelida, Clitellata) based on mitochondrial and nuclear sequence data","volume":"35","author":"Sjolin","year":"2005","journal-title":"Molecular Phylogenetics & Evolution"},{"key":"2020032801403549600_WST-EM16416R2C29","doi-asserted-by":"crossref","first-page":"3351","DOI":"10.1016\/j.watres.2011.03.046","article-title":"Municipal wastewater treatment and biomass accumulation with a wastewater-born and settleable algal-bacterial culture","volume":"45","author":"Su","year":"2011","journal-title":"Water Research"},{"key":"2020032801403549600_WST-EM16416R2C30","doi-asserted-by":"crossref","first-page":"5639","DOI":"10.1016\/j.biortech.2011.02.054","article-title":"Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent","volume":"102","author":"Wang","year":"2011","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C32","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.biortech.2013.05.058","article-title":"Integration process of biodiesel production from filamentous oleaginous microalgae Tribonema minus","volume":"142","author":"Wang","year":"2013","journal-title":"Bioresource Technology"},{"key":"2020032801403549600_WST-EM16416R2C33","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.chemosphere.2013.03.014","article-title":"Nitrogen removal from Lake Caohai, a typical ultra-eutrophic lake in China with large scale confined growth of Eichhornia crassipes","volume":"92","author":"Wang","year":"2013","journal-title":"Chemosphere"},{"key":"2020032801403549600_WST-EM16416R2C34","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.hal.2011.03.002","article-title":"Temporal variability of cyanobacterial populations in the water and sediment samples of Lake Taihu as determined by DGGE and real-time PCR","volume":"10","author":"Ye","year":"2011","journal-title":"Harmful Algae"},{"key":"2020032801403549600_WST-EM16416R2C35","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1128\/AEM.02240-08","article-title":"Generation of electricity and analysis of microbial communities in wheat straw biomass-powered microbial fuel cells","volume":"75","author":"Zhang","year":"2009","journal-title":"Applied & Environmental Microbiology"},{"key":"2020032801403549600_WST-EM16416R2C36","doi-asserted-by":"crossref","first-page":"4294","DOI":"10.1016\/j.watres.2013.05.004","article-title":"Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment","volume":"47","author":"Zhu","year":"2013","journal-title":"Water Research"}],"container-title":["Water Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/iwaponline.com\/wst\/article-pdf\/74\/11\/2727\/457521\/wst074112727.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/iwaponline.com\/wst\/article-pdf\/74\/11\/2727\/457521\/wst074112727.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T06:11:59Z","timestamp":1585375919000},"score":1,"resource":{"primary":{"URL":"https:\/\/iwaponline.com\/wst\/article\/74\/11\/2727\/18973\/Identification-and-evaluation-of-a-dominant-alga"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,1]]},"references-count":36,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2016,10,1]]},"published-print":{"date-parts":[[2016,12,14]]}},"URL":"https:\/\/doi.org\/10.2166\/wst.2016.437","relation":{},"ISSN":["0273-1223","1996-9732"],"issn-type":[{"value":"0273-1223","type":"print"},{"value":"1996-9732","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016,12,14]]},"published":{"date-parts":[[2016,10,1]]}}}