{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:35:09Z","timestamp":1762252509021},"reference-count":25,"publisher":"IWA Publishing","issue":"11","content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,6,15]]},"abstract":"<jats:p>Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 \u00b1 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 \u00b1 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 \u00b1 87 and 587 \u00b1 91 viable ova, respectively. The numbers of viable ova estimated by the culture-based method were significantly (P &amp;lt; 0.05) lower than vital stain and PMA-qPCR methods. Therefore, both PMA-qPCR and vital stain methods appear to be suitable for the quantitative detection of viable hookworm ova. However, PMA-qPCR would be preferable over the vital stain method in scenarios where ova speciation is needed.<\/jats:p>","DOI":"10.2166\/wst.2017.142","type":"journal-article","created":{"date-parts":[[2017,3,7]],"date-time":"2017-03-07T01:25:58Z","timestamp":1488849958000},"page":"2615-2621","update-policy":"http:\/\/dx.doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of culture-based, vital stain and PMA-qPCR methods for the quantitative detection of viable hookworm ova"],"prefix":"10.2166","volume":"75","author":[{"given":"P.","family":"Gyawali","sequence":"first","affiliation":[{"name":"School of Public Health, The University of Queensland, Herston Road, Herston, Qld 4006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P. 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