{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:55:57Z","timestamp":1785797757444,"version":"3.56.0"},"reference-count":32,"publisher":"IWA Publishing","issue":"1","content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,4,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Wastewater treatment plants (WWTPs) represent an important reservoir of antibiotic resistance determinants. Although many studies have been conducted to evaluate resistance profiles in Enterobacteriaceae isolates from this setting, the dynamics of this phenomenon are poorly known to the bacterium Pseudomonas aeruginosa. Here we aimed to evaluate the resistance profiles and the production of AmpC \u03b2-lactamase in P. aeruginosa isolates from a domestic full-scale WWTP. Samples of the raw sewage and effluent were collected and the bacterium P. aeruginosa was isolated on cetrimide agar. Susceptibility to \u03b2-lactams, fluoroquinolones and aminoglycosides was evaluated by the disc diffusion method, and the presence of AmpC \u03b2-lactamase was investigated phenotypically and by molecular method. We recovered 27 isolates of P. aeruginosa. Of these, 81.5% were susceptible to all antimicrobials tested. However, a considerable rate of resistance to carbapenems (11%) was found among the isolates. Twenty-two isolates were positive in the phenotypic test for inducible AmpC \u03b2-lactamase but the blaampc gene was only identified in four isolates, suggesting the presence of other independent resistance mechanisms besides this \u03b2-lactamase. In summary, we have shown that P. aeruginosa isolates from a domestic WWTP represents a potential reservoir of blaampC genes and other resistance determinants, including those that result in low susceptibility to carbapenems and aminoglycosides.<\/jats:p>","DOI":"10.2166\/wst.2018.091","type":"journal-article","created":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T01:20:13Z","timestamp":1519867213000},"page":"108-114","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":20,"title":["Antibiotic resistance profile and occurrence of AmpC between Pseudomonas aeruginosa isolated from a domestic full-scale WWTP in southeast Brazil"],"prefix":"10.2166","volume":"2017","author":[{"given":"Luiza Ger\u00e7ossimo","family":"Oliveira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Laboratorial e Microbiologia Cl\u00ednica, Campus Centro Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o del Rei, Divin\u00f3polis, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Gon\u00e7alves Resende","family":"Ferreira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Laboratorial e Microbiologia Cl\u00ednica, Campus Centro Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o del Rei, Divin\u00f3polis, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Maria Amaral","family":"Nascimento","sequence":"first","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana de Paula","family":"Reis","sequence":"first","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Fran\u00e7a","family":"Dias","sequence":"first","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Gustavo","family":"Lima","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia M\u00e9dica, Campus Centro Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o del Rei, Divin\u00f3polis, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magna Cristina","family":"Paiva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Laboratorial e Microbiologia Cl\u00ednica, Campus Centro Oeste Dona Lindu, Universidade Federal de S\u00e3o Jo\u00e3o del Rei, Divin\u00f3polis, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"945","published-online":{"date-parts":[[2018,2,28]]},"reference":[{"issue":"12","key":"2020032810511786200_WST-EM171514C1","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/cid\/ciu190","article-title":"Wastewater treatment plants release large amounts of extended-spectrum \u03b2-lactamase\u2013producing Escherichia coli into the environment","volume":"58","year":"2014","journal-title":"Clin. 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