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The results showed that the removal rate of ARG could reach 96.17% under the best preparation conditions of immobilized pellets (3% sodium alginate concentration, 0.7% corn straw biochar, 5% white rot fungus mycelium suspension, 4% CaCl2, and 36 h immobilization time). The orthogonal test results showed that the best combination was the immobilized pellets dosage of 200\/100 mL, pH of 4.5, rotation speed of 150 r\/min, and initial concentration of 20 mg\/L dye at 30 \u00b0C. The degradation pathway of ARG by immobilized microspheres was studied by ultraviolet-visible spectrometer, Fourier transform infrared spectroscopy, and liquid chromatography-mass spectrometry. The results showed that ARG was degraded into aniline and 5-(acetamino)-4-hydroxy-3-amino-2,7-naphthalene disulfonic acid. Aniline was further deaminated to form benzene, and benzene was ring opened to form other organic compounds; 5-(acetylamino)-4-hydroxy-3-amino-2,7-naphthalene disulfonic acid was dehydroxylated to form 5-(acetylamino)-3-amino-2,7-naphthalene disulfonic acid. This study shows that the prepared biochar immobilized pellets can be used as an efficient water treatment material to remove ARG dye from wastewater.<\/jats:p>","DOI":"10.2166\/wst.2022.155","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T14:12:46Z","timestamp":1652105566000},"page":"2945-2963","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":7,"title":["Study on treatment of acid red G with bio-carbon compound immobilized white rot fungi"],"prefix":"10.2166","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2918-0802","authenticated-orcid":false,"given":"Huifang","family":"Wu","sequence":"first","affiliation":[{"name":"1 College of Urban Construction, Nanjing Tech University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Xu","sequence":"additional","affiliation":[{"name":"1 College of Urban Construction, Nanjing Tech 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