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Sewage aliquots were inoculated in a selective medium with antibiotics. Bacterial identification was performed by MALDI-TOF and ARGs were assessed by polymerase chain reaction (PCR). A total of 208 strains from both hospitals were isolated (H1 = 117; H2 = 91). A wide variety of Enterobacterales and non-Enterobacterales species were isolated and most of them were Enterobacter spp. (13.0%), Proteus mirabilis (10.1%), and Klebsiella pneumoniae (9.6%). blaTEM and blaKPC were the most frequent \u03b2-lactamase-encoding genes and the predominant macrolide resistance genes were mph(A) and mel. Many species had the three tetracycline resistance genes (tetD, tetM, tetA) and strB was the prevalent aminoglycoside resistance gene. Two Staphylococcus haemolyticus strains had the mecA gene. Quinolone, colistin, and vancomycin resistance genes were not found. This study showed that hospital raw sewage is a great ARB and ARG disseminator. Strict monitoring of hospital sewage treatment is needed to avoid the spread of these genes among bacteria in the environment.<\/jats:p>","DOI":"10.2166\/wst.2022.427","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T17:14:52Z","timestamp":1672161292000},"page":"239-250","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":19,"title":["Diversity of bacteria carrying antibiotic resistance genes in hospital raw sewage in Southeastern Brazil"],"prefix":"10.2166","volume":"87","author":[{"given":"Marcos Paulo Barbosa","family":"Batista","sequence":"first","affiliation":[{"name":"a Department of Pathology, Health Sciences Center, Federal University of Esp\u00edrito Santo, Vit\u00f3ria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Sampaio","family":"Cavalcante","sequence":"additional","affiliation":[{"name":"b Campus Maca\u00e9, Federal University of Rio de 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