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This study aimed to assess the impact of different hydraulic retention times (HRT) on hydrogen production in a sequencing batch reactor system using raw cornWW as feedstock. Different HRTs were evaluated (4, 2, and 1 day(s)). Higher biohydrogen productivity was observed in HRT value of 1 day (893.6 \u00b1 10.1 NmL H2\/Lreactor\/day), indicating its favorable metabolic pathways leading to the generation of hydrogen, carbon dioxide, acetate, butyrate, and caproate. Microbial analysis revealed that the Atopobium and Clostridium (genera) played key roles in hydrogen and organic acid production. Additionally, during the fermentation of cornWW, lactic acid in the feedstock facilitated the production of caproic and propionic acids, further enriching the range of valuable byproducts obtained through this process.<\/jats:p>","DOI":"10.2166\/wst.2025.009","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T07:29:46Z","timestamp":1738049386000},"page":"321-331","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of hydraulic retention time on hydrogen production from corn industry wastewater by dark fermentation"],"prefix":"10.2166","volume":"91","author":[{"given":"Monserrat","family":"V\u00e1zquez-L\u00f3pez","sequence":"first","affiliation":[{"name":"a Laboratory for Research on Advanced Processes for Water Treatment, Unidad Acad\u00e9mica Juriquilla, Instituto de Ingenier\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Blvd. Juriquilla 3001, 76230 Quer\u00e9taro, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalinda","family":"Campuzano","sequence":"additional","affiliation":[{"name":"b Biotechnology Department, Universidad Aut\u00f3noma Metropolitana, Av. 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