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During rainfall events, these sediments can be resuspended and transported via runoff into storm and combined sewers. This study investigated the bacterial diversity and potential health hazards associated with resuspended road-deposited matter in a peri-urban area. Quantitative PCR and metabarcoding analyses of 16S rRNA and tpm genes were performed to (i) identify the sources of bacterial taxa colonizing road surfaces, (ii) define core and specific taxa and assess their capacity to survive in downstream sewer environments, and (iii) explore their functional potential. Several taxa were linked to human and animal sources, with notable occurrences of bacterial pathogen DNA signatures. Amplicon sequence variant profiling revealed that resuspended road surface communities were more similar to those in storm sewage than in combined sewage. 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